Showing 1-30 of 8546 results for "precipitation"
Yongsheng Ji et al.
Analytica chimica acta, 1053, 43-53 (2019-02-05)
Hydrophilic interaction chromatography (HILIC) utilizing zwitterion-modified material as solid phase has attracted extensive attention for selective enrichment of glycopeptides. However, a tedious synthesis and low specificity for glycopeptides have restricted its application. Herein, a facile and effective approach was developed...
Kosha B Shah et al.
International journal of pharmaceutics, 468(1-2), 64-74 (2014-04-09)
In current pharmaceutical drug discovery, most candidates are poorly soluble in water, which can result in poor bioavailability. To overcome this problem, formulations that create supersaturation of the drug are a well-studied alternative. Characterizing the dissolution from these systems is...
Bahngmi Jung et al.
Journal of environmental sciences (China), 74, 168-176 (2018-10-21)
This study investigates the removal of arsenite (As(III)) from water using dithionite activated by UV light. This work evaluated the removal kinetics of As(III) under UV light irradiation as affected by dithionite dose and light intensity, and characterized the nature...
Srinivas Chollangi et al.
Biotechnology and bioengineering, 112(11), 2292-2304 (2015-05-08)
To be administered to patients, therapeutic monoclonal antibodies must have very high purity, with process related impurities like host-cell proteins (HCPs) and DNA reduced to <100 ppm and <10 ppb, respectively, relative to desired product. Traditionally, Protein-A chromatography as a capture step...
Dennis Weidener et al.
Molecules (Basel, Switzerland), 25(15) (2020-07-28)
Fractionation of lignocellulose into its three main components, lignin, hemicelluloses, and cellulose, is a common approach in modern biorefinery concepts. Whereas the valorization of hemicelluloses and cellulose sugars has been widely discussed in literature, lignin utilization is still challenging. Due...
Blanca V Rodriguez et al.
Analytical biochemistry, 492, 69-75 (2015-09-30)
High-quality chromosomal DNA is a requirement for many biochemical and molecular biological techniques. To isolate cellular DNA, standard protocols typically lyse cells and separate nucleic acids from other biological molecules using a combination of chemical and physical methods. After a...
Xiaoyong Zhao et al.
Journal of separation science, 42(9), 1757-1767 (2019-02-28)
In the present work, three hydrophilic ionic liquids based on the combination between imidazolium cations attached with ethylene glycol polymers of various lengths and hexafluorophosphate anion were designed and synthesized for the separation of polysaccharides. By employing dextran 100 kDa as...
Yong Li et al.
International journal of pharmaceutics, 486(1-2), 207-216 (2015-04-07)
In this work, poorly water soluble phytochemical ellagic acid (EA) was micronized to increase its solubility and thereby the bioavailability during antisolvent precipitation process using N-methyl pyrrolidone (NMP) as solvent and deionized water as antisolvent. The micronized EA (m-EA) freeze-dried...
Taiga Uekusa et al.
Journal of pharmaceutical and biomedical analysis, 161, 45-50 (2018-08-26)
The purpose of the present study was to investigate the precipitation of a drug on the particle surface of its salt in biorelevant media. Pioglitazone (PIO, weak base, pKa = 5.8) was used as a model drug. The crystal particles of PIO...
Rahul D Sheth et al.
Biotechnology and bioengineering, 111(8), 1595-1603 (2014-03-07)
In this work, a proof of concept elastin-like polypeptide-Z domain fusion (ELP-Z) based affinity precipitation process is developed for monoclonal antibody (mAb) purification from industrial harvest feeds. Greater than 99% mAb recoveries are obtained during the initial binding step of...
Sangjun Jeon et al.
Journal of bioscience and bioengineering, 117(4), 464-470 (2013-10-23)
This study presents a new and effective downstream process to recover 2,3-butanediol (2,3-BD) from fermentation broth which is produced by a recombinant Klebsiella pneumoniae strain. The ldhA-deficient K. pneumoniae strain yielded about 90 g/L of 2,3-BD, along with a number...
Nabila Haddou et al.
Chemosphere, 107, 304-310 (2014-01-28)
The Gliding Arc Discharge (GAD) is an efficient non-thermal plasma technique able to degrade organic compounds dispersed in water at atmospheric pressure. The degradation of the organometallic lead acetate (PbAc) in aqueous solution was performed by two distinct plasmageneous processes:...
Kiseok Kim et al.
Biochemical and biophysical research communications, 448(1), 114-119 (2014-04-29)
Protein precipitation is one of the most widely used methods for antigen detection and purification in biological research. We developed a reproducible aptamer-mediated magnetic protein precipitation method that is able to efficiently capture, purify and isolate the target proteins. We...
Nikhil Kateja et al.
Biotechnology journal, 11(10), 1320-1331 (2016-07-23)
Coiled Flow Inverter Reactor (CFIR) has recently been explored for facilitating continuous operation of several unit operations involved in downstream processing of biopharmaceuticals such as viral inactivation and protein refolding. The application of CFIR for continuous precipitation of clarified cell...
Deepak Yadav et al.
International journal of pharmaceutics, 477(1-2), 564-577 (2014-12-03)
The present work aims to investigate applicability of antisolvent precipitation method for preparation of nanosized curcumin and to control their characteristics by determining the influence of process and solvents on solid-state properties of curcumin nanoparticles. Effects of different experimental parameters...
Mahmoud Seifi et al.
International journal of pharmaceutics, 471(1-2), 37-44 (2014-05-06)
In the present work a series of tramadole imprinted micro- and nanoparticles were prepared and study their recognition properties. Methacrylic acid (MAA), as a functional monomer, ethylene glycol dimethacrylate (EGDMA) as a cross-linker and different solvents (chloroform, toluene and acetonitrile...
S S Mohtar et al.
Bioresource technology, 192, 212-218 (2015-06-04)
The objective of this study is to extract and characterize lignin from oil palm biomass (OPB) by dissolution in 1-butyl-3-methylimidazolium chloride ([bmim][Cl]), followed by the lignin extraction through the CO2 gas purging prior to addition of aluminum potassium sulfate dodecahydrate...
Y SUGINO et al.
The Journal of biological chemistry, 239, 2360-2364 (1964-07-01)
Emma-Tuulia Tolonen et al.
Journal of environmental management, 181, 856-862 (2016-07-12)
The aim of this research was to investigate sulphate removal from mine water by precipitation as ettringite (Ca6Al2(SO4)3(OH)12·26H2O) and the utilisation of the precipitate as a sorbent for arsenate removal. The mine water sulphate concentration was reduced by 85-90% from...
Fan Yang et al.
Biosensors & bioelectronics, 55, 360-365 (2014-01-15)
A novel sandwich-type electrochemical immunosensor based on functionalized nanomaterial labels and bienzyme (horseradish peroxidase and glucose oxidase) biocatalyzed precipitation was developed for the detection of α-fetoprotein (AFP). The enzymes linked to functionalized nanomaterials as biocatalysts could accelerate the oxidation of...
Shunsuke Izaki et al.
Journal of pharmaceutical sciences, 104(6), 1929-1937 (2015-03-27)
Development of high-concentration antibody formulations for subcutaneous administration remains challenging. Recently, a precipitation-redissolution method was proposed to prepare suspensions or precipitates of salt-dissociable protein-poly(amino acid) complexes. To elucidate the utility of this method for protein therapy, we investigated the feasibility...
J L Zhang et al.
Applied microbiology and biotechnology, 100(24), 10295-10306 (2016-07-28)
A strategy to supply molecular oxygen for microbial calcium precipitation was developed for the first time. Firstly, a controlled oxygen-releasing tablet (ORT) containing CaO
Fotios Baxevanis et al.
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 131, 178-188 (2018-08-16)
In this study, the influence of the presence of excipients in sample preparation and clean-up steps required prior to drug analysis in milk-based media which simulate the in vivo properties of the fed state stomach was investigated. 15 excipients, normally...
Katherine Margulis et al.
Journal of colloid and interface science, 434, 65-70 (2014-08-30)
Nanometric particles of a model hydrophobic substance curcumin were prepared by a novel method, namely, flash nanoprecipitation from a coarse oil-in-water emulsion. The method employs turbulent co-mixing of water with curcumin-loaded emulsion using manually-operated confined impingement jets mixer. A clear...
Steven D Branston et al.
Biotechnology and bioengineering, 112(8), 1714-1719 (2015-03-03)
The Ff filamentous bacteriophages show potential as a new class of therapeutics, displaying utility in materials science as well as pharmaceutical applications. These phages are produced by the infection of E. coli, a Gram-negative bacterium which unavoidably sheds endotoxins into...
Iain Wilson et al.
Bulletin of environmental contamination and toxicology, 98(5), 601-606 (2017-03-04)
Effects caused in ecotoxicity tests by physical factors due to precipitated particles cannot be used for classification in Europe under the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH). For tin (Sn), results from ecotoxicity tests have been observed to...
Yang Lei et al.
Water research, 142, 10-17 (2018-05-29)
To address the issues of eutrophication and the potential risk of phosphorus (P) shortage, it is essential to remove and recover P from P-containing streams to close this nutrient cycle. Electrochemical induced calcium phosphate (CaP) precipitation was shown to be...
Rivca L Valk-Weeber et al.
Carbohydrate research, 479, 13-22 (2019-05-18)
Glycoproteins are biologically active proteins of which the attached glycans contribute to their biological functionality. Limited data is available on the functional properties of these N-glycans in isolation, without the protein core. Glycan release, typically performed with the PNGase F...
Rana Al-Shaikh Hamid et al.
Marine drugs, 8(5), 1699-1715 (2010-06-19)
The performance of the novel chitin metal silicate (CMS) co-precipitates as a single multifunctional excipient in tablet formulation using direct compression and wet granulation methods is evaluated. The neutral, acidic, and basic drugs Spironolactone (SPL), ibuprofen (IBU) and metronidazole (MET)...
Kristina Kösters et al.
Journal of chromatography. A, 1624, 461258-461258 (2020-06-17)
Lithium ion batteries are essential power sources in portable electronics, electric vehicles and as energy storage devices for renewable energies. During harsh battery cell operation as well as at elevated temperatures, the electrolyte decomposes and inter alia organo(fluoro)phosphates are formed...

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