Showing 1-30 of 458 results for "precipitation"
Precipitation Procedures
This page describes protein precipitation as an optional step in sample preparation for 2-D electrophoresis with Cytiva products.
Fractional Precipitation Protocol for Affinity Chromatography Samples
This page shows describes fractional precipitation for removing gross impurities from affinity chromatography samples with small volumes.
Common Cell Culture Problems: Precipitates
Common Cell Culture Problems: Precipitates -- When contamination is ruled out, turbidity in cell culture media is often explained by the precipitation of metals, proteins, and other media components. Precipitates can be harmful to cell health, as they may alter...
LC/MS/MS Analysis Using On-line Cartridges for the Removal of Phospholipids from Protein Precipitated Biological Fluid Samples
Learn more about LC/MS/MS Analysis using on-line cartridges for the removal of phospholipids from protein precipitated biological fluid samples
Sample Preparation for Size Exclusion Chromatography
Samples for chromatographic purification should be clear and free from particulate matter. Simple steps to clarify a sample before beginning purification will avoid clogging the column, can reduce the need for stringent washing procedures, and can extend the life of...
Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules
Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules
HybridSPE-Phospholipid Technology
This Sigma-Aldrich article discusses how the HybridSPE-Phospholipid Technology works and how the phospholipids are removed.
Sample Preparation in Ion Exchange Chromatography
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
Sample Preparation for Chromatographic Purification
This page discusses various aspects of sample preparation for chromatographic purification.
Scaling Up High-Energy Cathode Materials for Electric Vehicles
The critical technical challenges associated with the commercialization of electric vehicle batteries include cost, performance, abuse tolerance, and lifespan.
Media Preparation – Plant Tissue Culture Protocol
Preparation from Packaged Powder, Preparation from Basal Salt Solutions, Banana Powder Preparation and Use, Coconut Water Preparation and Use
LC/MS/MS Analysis of Steroid Hormones in Plasma
The conversion of clinical methods to LC/MS/MS offers advantages; however, is accompanied by a few limitations, notably interference effects from the endogenous sample matrix.
Rapid, Sensitive, and Quantitative LC/MS/MS Determination of Digitoxin and Digoxin in Plasma
Immunoassay-based methods for digitoxin or digoxin exist, but are time consuming, labor intensive, and not selective due to similarities in their chemical structures.
Vitamin D epi-Metabolites Separated with HybridSPE-Phospholipid
This Sigma-Aldrich article continues to detail new methodology for the analysis of Vitamin D metabolites using HybridSPE-Phospholipid technology.
Purification of Serum Peptides for MALDI Mass Spectrometry with Amicon® Ultra Centrifugal Filters
Biomarkers play an essential role in the drug discovery and development process.
Crystallization
The elucidation of a macromolecular structure to the atomic level by X-ray or neutron diffraction analysis requires the molecule to be available in the form of relatively large single crystals. Many soluble proteins, membrane proteins, nucleic acids and nucleoprotein complexes...
Two Distinct Sample Prep Approaches to Overcome Matrix Effect in LC/MS of Serum or Plasma Samples
This article highlights the impact that sample matrix effects can have on LC/MS response and discusses two novel approaches to reduce it.
Improvement in LC/MS Analysis of Vitamin D Metabolites in Serum by Leveraging Column Selectivity and Effective Sample Prep
Improvement in LC/MS Analysis of Vitamin D Metabolites in Serum by Leveraging Column Selectivity and Effective Sample Prep
Protocol for Conjugating NHS-Ester Modifications to Amino-Labeled Oligonucleotides
This protocol is for conjugating NHS-ester modifications to an oligonucleotide with an amino label, such as 5'-Amino-Modifier C6.
Cleaning Up Samples using 2-D Clean-Up Kit
2-D Clean-Up Kit from Cytiva is designed to prepare samples that would otherwise produce poor 2-D results.
Method Optimization for LC-MS Analysis of Vitamin D Metabolite Critical Pairs in Serum
Using HybridSPE-Phospholipid, Ascentis Express, and Other LC-MS Workflow Components
Method Optimization for LC-MS Analysis of Vitamin D Metabolite Critical Pairs in Serum
Novel sample prep technique coupled with the unique selectivity of the Ascentis Express F5 column enables a fast and simplified bioanalytical method for associated vitamin D metabolites.
Ion-Suppression & Phospholipid Contamination
We are presenting an article focusing on ion-suppression and phospholipid contamination and some of their major causes and difficulties.
Chiral and Achiral LC-MS Analysis of Warfarin™ in Plasma Samples
Clinical laboratories are beginning to reap the benefits of LC-MS in terms of its sensitivity, specificity, throughput, and potential to reduce cost per sample. The purpose of this study was to demonstrate the LC-MS analysis of Warfarin in plasma samples...
Plasmid DNA Purification
Plasmids purified from genomic DNA, proteins, ribosomes, and the bacterial cell wall are used in molecular biology research. Various methods have been developed for plasmid DNA purification.
Ion Exchange Chromatography
Ion exchange chromatography is employed by the Genopure Plasmid Midi Kit and the Genopure Plasmid Maxi Kit.
Investigating Matrix Interference in Analysis of Antiarrhythmic Cardiac Drugs in Plasma
For efficient therapeutic drug monitoring, it is important for clinicians to have access to fast and robust analytical methods for accurate assessment of drug efficacy. Industrial trends toward highly specific LC/MS applications over traditional immunoassay have resulted in the need...
Fast and Accurate Analysis of Vitamin D Metabolites Using Novel Chromatographic Selectivity and Sample Preparation
Vitamin D deficiency has become a topic of interest in recent publications. Vitamin D regulates the absorption of calcium within the digestive track and is important to promote proper bone growth and prevent osteoprosis in older adults.
DNA / Protein Electrophoresis and Troubleshooting Tables
DNA / Protein Electrophoresis and Troubleshooting Tables
NBT Protocol & Troubleshooting
There are several counterstains possible in combination with BM Purple (or NBT/BCIP in general), including FastGreen FCF and Nuclear Fast Red.

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