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Specific Detection of Pseudomonas aeruginosa in Water
24)
• Prefilled TSA Milliflex cassettes
(Cat. no. MXSM CTS 48)
• Prefilled R2A Milliflex cassettes
(Cat. no. MXSM CRA 48)
• Sterile 0.9% NaCl water
Milliflex Oasis Sustainability Sheet
the media plate for
incubation, we classify them as bio-hazardous waste.
Milliflex Oasis® media plate components:
All the components of the media plate are in contact with the
culture media and
Milliflex Quantum Rapid Detection System
Preparation
Filter the desired sample volume Milliflex Oasis®
presterilized, disposable Milliflex® filter units.
Place membrane filter base onto a prefilled agar
cassette and incubate
2. Fluorescent
Milliflex Quantum Rapid Detection System
through the
window of the Milliflex® Quantum reader
or use the camera to view the colonies
on your computer screen.
Reincubation Step
Place membrane on a prefilled agar media cassette and reincubate
Milliflex®Quantum Rapid Detection System
through the
window of the Milliflex® Quantum reader
or use the camera to view the colonies
on your computer screen.
Reincubation Step
Place membrane on a prefilled agar media cassette and reincubate
Rapid Sterility Testing of Filterable Samples
preparation is performed in triplicate, the samples are filtered using the Milliflex Plus pump, and
the membranes are transferred onto prefilled, gamma-irradiated rapid sterility testing medium (RSTM;
Figure 2
Reprint - White Paper: Milliflex® Quantum - Detection of microbial contaminants in water samples
water sample was filtered through a Milliflex® 0.45 µm MCE membrane using the Milliflex®
PLUS pump system. The membrane was placed onto a prefilled R2A Milliflex® agar media cassette
FRESH PERSPECTIVES
MIP AN Milliflex Quantum Sample Matrices
filtered through a Milliflex® 0.45 μm
MCE membrane using the Milliflex® PLUS pump system.
The membrane was rinsed with rinse fluid and placed
onto a prefilled Millifl ex® agar media cassette and
incubated
Milliflex Rapid System Equivalency Assessment
is then transferred onto a media plate and incubated for growth.
Step 2: Apply reagents
After incubation, remove the membrane filter from the Milliflex Oasis® prefilled agar plate using the membrane
Milliflex Quantum System - Water Samples
sample was filtered through a Milliflex®
0.45 μm MCE membrane using the Milliflex® PLUS
pump system. The membrane was placed onto a
prefilled R2A Milliflex® agar media cassette and
incubated at 32.5
Milliflex®Rapid Microbiology Detection System
2: Apply Reagents
Release filter base from the Milliflex® prefilled
media cassette and place filter unit on the
Milliflex® AutoSpray Station for application of
reagents. Reagents are automatically
Datasheet - Milliflex Rapid System
incubation, remove the membrane filter from
the Milliflex Oasis® prefilled agar plates using the
membrane removal tool and place the membrane filter
on the Milliflex® Rapid 2.0 AutoSpray Station to apply
the
User Guide Milliflex Oasis System
MMHVMFX24
Milliflex Oasis® funnel without membrane, growth promotion accessory 24 MMRECVERY
Culture media
Milliflex Oasis® R2A low nutrient media plates 48 MMSMCRA48
Milliflex Oasis® Tryptic Soy media plates
Datasheet Milliflex Oasis System
MMHVMFX24
Milliflex Oasis® funnel without membrane, growth promotion accessory 24 MMRECVERY
Culture media
Milliflex Oasis® R2A low nutrient media plates 48 MMSMCRA48
Milliflex Oasis® tryptic soy media plates
Milliflex Quantum: A Fluorescence-based Platform for the Rapid Detection of Contaminants in Filterable Products
microcolonies with
the Milliflex Quantum reader
Reincubation
on media for
identification
Membrane transfer on
Milliflex solid
media cassette
Membrane transfer on Milliflex liquid
cassette with
Milliflex Oasis Validation Summary
the Milliflex® system on all the tested parameters.
1.2 Product description
1.2.1 Media plate
1. Milliflex Oasis® Product Summary
Three culture media types are available in the Milliflex Oasis
Milliflex Oasis and Milliflex Quantum systems for rapid and quantitative microbial detection
nutrient media plates MMSMCRAS48
Milliflex Oasis® SDA media plates MMSMCSD48
Milliflex® liquid media cassette MXLMC0120
Milliflex® Quantum rapid detection system MMQUANK01
Milliflex® Quantum
Bioburden Testing Solutions from - Simple, Secure, Complete.
12
Milliflex® Pumps, Funnels, and Media Cassettes
Description Qty/Pk Catalog No.
Milliflex® PLUS Pump
Milliflex® PLUS Pump single head kit 1 MXPPLUS01
Milliflex® PLUS Pump double
Milliflex Quantum Disinfectants Testing
0.45 μm
MMHVWP124
Milliflex Oasis® tryptic soy media plates for
detection of aerobic and anaerobic flora
MMSMCTS48
Milliflex® Liquid Media Cassette MXLMC0120
Milliflex® Quantum Reagent Kit
User Guide - Milliflex Oasis VHP Pump
for Milliflex Oasis®
VHP Pump (MMSANKITCVR)
Consumables
Please consult the Ordering Information section
for details on filtration units, culture media, and
consumables for the Milliflex Oasis
Application Note: Monitoring Microbial Contamination of Mammalian Cell Cultures
microbial culture medium developed for rapid
testing of various product types. Subsequent incubation
was performed as stated in Table 1 above.
Milliflex® Rapid detection
Following the Milliflex® protocol
Unmatched comfort In microbial testing with the Milliflex Oasis system
MMHVMFX24
Milliflex Oasis® funnel without membrane, growth promotion accessory 24 MMRECVERY
Culture media
Milliflex Oasis® R2A low nutrient media plates 48 MMSMCRA48
Milliflex Oasis® tryptic
User Guide-SYMBSYS01
Installing the Steritest™ EZ Device for Prefilled Syringes
Pre-Wetting the Membranes
Testing the Product
Rinsing the Tubing, Canisters, and Membranes
Adding Media to the Canisters
Maintaining
Milliflex Oasis System User Guide for use with EZ-Fit Filtration Units
Filtration units
2. Culture media
3. Consumables for the Milliflex Oasis® internal flow sanitization kit
Page 5 of 24
1.2 Item description
The Milliflex Oasis® pump for
Upstream process intensification using frozen high cell density intermediates
tube for prefilling of the cryo medium as well as a
connecting tube for a bioreactor.
For filling, 50 mL cryo medium with a concentrated DMSO-
concentration of 22.5 % need to be prefilled in the bags
Suitability of fluorescence-based microbial detection technology for pharmaceutical applications
Raw materials (media, buffers, pharmaceutical
ingredients and water)
• In-process samples (bioburden prior to sterilization,
CIP/SIP samples, cell culture/fermentation samples,
media for fermentation
Comparative Analysis half-Fraser Readybag Listeria
also a granulated
culture media, but provided in
prefilled, gamma irradiated pouches
of 12.5 g or 62.0 g. The required size
depends on the detection method.
Granulated culture media leads to
easier
Bacteria Application Note - Assessing the Level of Bacteria in Purified Water
maintained until culture.
Culture
Cultures should be done on an appropriate growth medium. Indeed, bacteria in purified water are stressed by
the poor conditions of their environment. Selected media, when combined
AppNote Readybag Listeria Stability Trial
granulated
culture media, provided in prefilled, gamma irradiated
pouches with a three-year shelf life. The food testing
routine is simplified because all that is required is the
addition of sterile water before
Validation of Milliflex Quantum for bioburden testing of pharmaceutical products - PDA Journal of Pharmaceutical Science and Technology 2017 71 206-224 (Oliver Gordon et al)
● Removal rack with liquid media cassette adapter
(REMRACK01, REMSPARE1)
● Milliflex® Plus Pump (MXPPLUS01)
● Milliflex® Plus Pump Head (MXPHEAD01)
● Milliflex® cassettes with tryptic soy agar
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