Showing 1-30 of 159219 results for "protein kinase"
Jennifer L Whiting et al.
The Journal of biological chemistry, 290(32), 19445-19457 (2015-06-20)
The proximity of an enzyme to its substrate can influence rate and magnitude of catalysis. A-kinase anchoring protein 220 (AKAP220) is a multivalent anchoring protein that can sequester a variety of signal transduction enzymes. These include protein kinase A (PKA)...
Bret K Samelson et al.
The Journal of biological chemistry, 290(22), 14107-14119 (2015-04-18)
Anchoring proteins direct protein kinases and phosphoprotein phosphatases toward selected substrates to control the efficacy, context, and duration of neuronal phosphorylation events. The A-kinase anchoring protein AKAP79/150 interacts with protein kinase A (PKA), protein kinase C (PKC), and protein phosphatase...
Lorena Buitrago et al.
The Journal of biological chemistry, 288(40), 29160-29169 (2013-08-21)
Protein kinase C (PKC) isoforms differentially regulate platelet functional responses downstream of glycoprotein VI (GPVI) signaling, but the role of PKCs regulating upstream effectors such as Syk is not known. We investigated the role of PKC on Syk tyrosine phosphorylation...
Jen-Kuan Chang et al.
The Journal of biological chemistry, 292(23), 9523-9539 (2017-04-15)
Although PKC-mediated phosphorylation of protein kinase D1 (PKD1) has been extensively characterized, little is known about PKD1 regulation by other upstream kinases. Here we report that stimulation of epithelial or fibroblastic cells with G protein-coupled receptor agonists, including angiotensin II...
Peifu Zhou et al.
Biochemical and biophysical research communications, 467(2), 421-426 (2015-09-30)
Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis (TB), has inflicted about one third of mankind and claims millions of deaths worldwide annually. Signalling plays an important role in Mtb pathogenesis and persistence, and thus represents attractive resource for drug...
Sayomi Higa-Nakamine et al.
The Journal of biological chemistry, 290(43), 25974-25985 (2015-09-05)
The receptor for gonadotropin-releasing hormone (GnRH) belongs to the G protein-coupled receptors (GPCRs), and its stimulation activates extracellular signal-regulated protein kinase (ERK). We found that the transactivation of ErbB4 was involved in GnRH-induced ERK activation in immortalized GnRH neurons (GT1-7...
A Jane Bardwell et al.
The Journal of biological chemistry, 290(44), 26661-26674 (2015-09-16)
MAPKs bind to many of their upstream regulators and downstream substrates via a short docking motif (the D-site) on their binding partner. MAPKs that are in different families (e.g. ERK, JNK, and p38) can bind selectively to D-sites in their...
Toshiaki Tanaka et al.
The FEBS journal, 281(23), 5237-5250 (2014-09-23)
The exocyst complex, also called the Sec6/8 complex, is important for targeting exocytic vesicles to specific docking sites on the plasma membrane in yeast and mammalian cells. In addition to these original findings, recent results of studies suggest that Sec8...
Sacnicte Ramírez Ríos et al.
Biochimica et biophysica acta, 1837(8), 1271-1283 (2014-04-15)
AMP-activated protein kinase (AMPK) and cytosolic brain-type creatine kinase (BCK) cooperate under energy stress to compensate for loss of adenosine triphosphate (ATP) by either stimulating ATP-generating and inhibiting ATP-consuming pathways, or by direct ATP regeneration from phosphocreatine, respectively. Here we...
Christina Servas et al.
Cellular signalling, 36, 163-175 (2017-05-11)
Apoptosis and the response to cell stress are evolutionary highly conserved mechanisms. Both processes require strict regulation, which is often performed by protein kinases. The mammalian Sterile 20-like kinase 1 (MST1) is a pro-apoptotic protein kinase, which is activated and...
Lawrence O Olala et al.
Molecular and cellular endocrinology, 392(1-2), 173-181 (2014-05-27)
Recent evidence has shown a role for the serine/threonine protein kinase D (PKD) in the regulation of acute aldosterone secretion upon angiotensin II (AngII) stimulation. However, the mechanism by which AngII activates PKD remains unclear. In this study, using both...
Carter J Swanson et al.
The Journal of biological chemistry, 289(25), 17812-17829 (2014-05-03)
Signaling proteins comprised of modular domains have evolved along with multicellularity as a method to facilitate increasing intracellular bandwidth. The effects of intramolecular interactions between modular domains within the context of native proteins have been largely unexplored. Here we examine...
Xueliang Gao et al.
The Journal of biological chemistry, 288(22), 15971-15979 (2013-04-12)
Pyruvate kinase isoform M2 (PKM2) is an enzyme-catalyzing conversion of phosphoenolpyruvate to pyruvate in the glycolysis pathway. It was demonstrated that PKM2 interacts with tyrosine phosphopeptide, and the interaction with the tyrosine phosphopeptide affects the pyruvate kinase activity of PKM2....
Yuan-Chii G Lee et al.
Phytomedicine : international journal of phytotherapy and phytopharmacology, 55, 255-263 (2019-01-23)
We previously showed that 3-O-β-D-glucopyranosyl-(3R)-hydroxybutanolide (kinsenoside), a major compound of Anoectochilus formosanus, increased lipolysis through an AMP-activated protein kinase (AMPK)-dependent pathway. To extend our previous finding, we investigated the in vivo and in vitro effects of kinsenoside on lipolysis and...
Hiroaki Tanaka et al.
The Journal of biological chemistry, 288(21), 15240-15254 (2013-04-04)
Proteins with Src homology 2 (SH2) domains play major roles in tyrosine kinase signaling. Structures of many SH2 domains have been studied, and the regions involved in their interactions with ligands have been elucidated. However, these analyses have been performed...
Jia Wang et al.
The Journal of biological chemistry, 291(34), 17988-18005 (2016-07-03)
We examined the regulation of Yes-associated protein (YAP) localization, phosphorylation, and transcriptional activity in intestinal epithelial cells. Our results show that stimulation of intestinal epithelial IEC-18 cells with the G protein-coupled receptor (GPCR) agonist angiotensin II, a potent mitogen for...
Sho Kubota et al.
The Journal of biological chemistry, 288(24), 17871-17883 (2013-05-07)
Protein tyrosine phosphorylation regulates a wide range of cellular processes at the plasma membrane. Recently, we showed that nuclear tyrosine phosphorylation by Src family kinases (SFKs) induces chromatin structural changes. In this study, we identify KRAB-associated protein 1 (KAP1/TIF1β/TRIM28), a...
Romina G Díaz et al.
European journal of pharmacology, 849, 96-105 (2019-02-06)
Since the original description as potent antianginal compounds, phosphodiesterase 5A inhibitors have continuously increased their possible therapeutic applications. In the heart, Sildenafil was shown to protect against an ischemic insult by decreasing cardiac Na+/H+ exchanger (NHE1) activity, action that was...
Xiaoqing Zhu et al.
The Journal of biological chemistry, 291(33), 17020-17027 (2016-07-15)
AMP-activated protein kinase (AMPK) is a molecular energy sensor that acts to sustain cellular energy balance. Although AMPK is implicated in the regulation of a multitude of ATP-dependent cellular processes, exactly how these processes are controlled by AMPK as well...
Erin M Leonard et al.
Experimental physiology, 104(2), 244-253 (2018-11-21)
What is the central question of this study? The mammalian carotid body (CB) is a peripheral chemoreceptor organ involved in O2 and CO2 /H+ homeostasis. Recent studies suggest that 5-HT, released from CB receptor cells, can stimulate adjacent glial-like type II...
C Blake Nichols et al.
Circulation research, 114(9), 1398-1409 (2014-03-20)
Both β-adrenergic receptor (β-AR) and Gq-coupled receptor (GqR) agonist-driven signaling play key roles in the events, leading up to and during cardiac dysfunction. How these stimuli interact at the level of protein kinase D (PKD), a nodal point in cardiac...
Shohreh Majd et al.
The European journal of neuroscience, 44(3), 1987-1997 (2016-05-15)
Abnormal tau phosphorylation (p-tau) has been shown after hypoxic damage to the brain associated with traumatic brain injury and stroke. As the level of p-tau is controlled by Glycogen Synthase Kinase (GSK)-3β, Protein Phosphatase 2A (PP2A) and Adenosine Monophosphate Kinase...
Anastasiya Strembitska et al.
International journal of molecular sciences, 19(12) (2018-12-20)
Protein kinase B (Akt) is a key enzyme in the insulin signalling cascade, required for insulin-stimulated NO production in endothelial cells (ECs). Previous studies have suggested that AMP-activated protein kinase (AMPK) activation stimulates NO synthesis and enhances insulin-stimulated Akt activation...
Ward G Walkup et al.
The Journal of biological chemistry, 290(8), 4908-4927 (2014-12-24)
synGAP is a neuron-specific Ras and Rap GTPase-activating protein (GAP) found in high concentrations in the postsynaptic density (PSD) fraction from the mammalian forebrain. We have previously shown that, in situ in the PSD fraction or in recombinant form in...
Ayumu Takeno et al.
International journal of molecular sciences, 19(6) (2018-06-16)
Phloretin, a glucose transporter (GLUT) inhibitor, has pleiotropic effects. The present study examined the effects of phloretin on the commitment of marrow stromal cells to adipocytes, using the mouse marrow stromal cell line ST2. Oil red O staining showed that...
Cinzia Franchin et al.
Biochimica et biophysica acta, 1854(6), 609-623 (2014-10-04)
CK2 is an extremely pleiotropic Ser/Thr protein kinase, responsible for the generation of a large proportion of the human phosphoproteome and implicated in a wide variety of biological functions. CK2 plays a global role as an anti-apoptotic agent, a property...
Mayu Kamimura et al.
Bioscience, biotechnology, and biochemistry, 78(3), 438-447 (2014-07-19)
Protein kinases are key regulators of cell function that constitute one of the largest and most functionally diverse gene families. We developed a novel assay system, based on the bimolecular fluorescence complementation (BiFC) technique in Escherichia coli, for detecting transient...
T De Wit et al.
Molecular neurobiology, 56(8), 5273-5286 (2018-12-29)
Mutations and variations in the leucine-rich repeat kinase 2 (LRRK2) gene are strongly associated with an increased risk to develop Parkinson's disease (PD). Most pathogenic LRRK2 mutations display increased kinase activity, which is believed to underlie LRRK2-mediated toxicity. Therefore, major...
Yong-Bae Kim et al.
The Journal of biological chemistry, 290(34), 21076-21085 (2015-07-15)
Upon growth factor stimulation, PAK1 is recruited to the plasma membrane and activated by a mechanism that requires its phosphorylation at Ser-223 by the protein kinase CK2. However, the upstream signaling molecules that regulate this phosphorylation event are not clearly...
Mathias Cobbaut et al.
FEBS letters, 592(14), 2432-2443 (2018-06-23)
The protein kinase D (PKD) family is regulated through multi-site phosphorylation, including autophosphorylation. For example, PKD displays in vivo autophosphorylation on Ser-742 (and Ser-738 in vitro) in the activation loop and Ser-910 in the C-tail (hPKD1 numbering). In this paper...

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