Showing 1-25 of 25 results for "R2153"
W G Luttge et al.
Endocrinology, 125(3), 1194-1203 (1989-09-01)
It is often implied that the various molecular, physiological, and behavioral responses to the glucocorticoid dexamethasone (DEX) are mediated in brain exclusively via the interactions of this synthetic steroid with the classical glucocorticoid (type II) receptor. The results reported in...
L A Rollins-Smith et al.
Developmental immunology, 5(2), 145-152 (1997-01-01)
In recent years, integrative animal biologists and behavioral scientists have begun to understand the complex interactions between the immune system and the neuroendocrine system. Amphibian metamorphosis offers a unique opportunity to study dramatic hormone-driven changes in the immune system in...
M Kalimi et al.
The American journal of physiology, 258(5 Pt 1), E737-E739 (1990-05-01)
The effect of mineralocorticoid antagonist RU 26752 on the development and maintenance of hypertension produced by long-term administration of mineralocorticoid agonist aldosterone has been investigated. Uninephrectomized, saline-drinking male Sprague-Dawley rats were subcutaneously implanted with either placebo (control) pellets or pellets...
M K Agarwal et al.
Renal physiology and biochemistry, 14(6), 217-223 (1991-11-01)
A number of chemical modifications in the spironolactone molecule were attempted over the last decade to synthesize ligands with high affinity for the mineralocorticoid receptor (MCR), and for possible use in the clinical control of the hypertensive disease. ZK 91587...
Cathy Le Moëllic et al.
Journal of the American Society of Nephrology : JASN, 15(5), 1145-1160 (2004-04-22)
Effects of aldosterone on its target cells have long been considered to be mediated exclusively through the genomic pathway; however, evidence has been provided for rapid effects of the hormone that may involve nongenomic mechanisms. Whether an interaction exists between...
M Mirshahi et al.
Biochemical and biophysical research communications, 219(1), 150-156 (1996-02-06)
Immunoblotting with a polyclonal antibody, directed against the mineralocorticoid receptor protein purified from rat kidney in presence of the receptor-specific ligand RU 26752, labeled a single 98-102 kDa band in soluble extracts from bovine retina and from cultured bovine retinal...
K Ben Rhouma et al.
Archives of physiology and biochemistry, 105(2), 216-224 (1997-04-01)
The thymolytic action of dexamethasone (DEXA) and aldosterone (ALDO) has been studied in vitro and in vivo. In vitro, in the presence of DEXA, the number of apoptotic cells increased with time. After 6 hours of incubation, 55 and 86%...
L Jacobson et al.
Brain research, 625(1), 84-92 (1993-10-15)
It is unclear whether in vitro corticosteroid receptor binding assays have used inappropriately high concentrations of synthetic corticosteroid competitors, thereby potentially introducing error into estimates of type I (mineralocorticoid) and type II (glucocorticoid) receptor binding. To determine more accurately the...
M Mirshahi et al.
Neuroendocrinology, 65(1), 70-78 (1997-01-01)
We studied the presence of the mineralocorticoid receptor (MCR) in the eye with the aid of a number of immunochemical techniques. Immunoblotting with a polyclonal antibody, directed against the rat renal MCR, revealed a single band of about 102 kD...
M K Agarwal et al.
Biochemical and biophysical research communications, 200(3), 1230-1238 (1994-05-16)
Total protein from N. tabacum cell cytosol, partially purified by ammonium sulphate precipitation, contains a 52 kDa protein that tests positive for mineralocorticoid receptor (MCR)-like activity by Western blots, immuneprecipitation and photochemical cross-linking. Binding of RU 26752, a ligand specific...
C B Whorwood et al.
Endocrinology, 135(3), 901-910 (1994-09-01)
The induction of Na,K-ATPase plays a vital role in mediating epithelial sodium transport. Although its activity is regulated by corticosteroids, it is uncertain whether this is predominantly by mineralo- or glucocorticoid mechanisms. 11 beta-Hydroxysteroid dehydrogenase (11 beta HSD) catalyzes the...
M Mirshahi et al.
Biochemical and biophysical research communications, 270(3), 811-815 (2000-04-25)
PCR analysis and Western blotting revealed the expression of the mineralocorticoid receptor (MCR) and the epithelial sodium channel (ENaC) genes at the level of RNA, DNA, and protein in several leukemic cell lines, fibroblasts from human cornea, and epithelial cells...
Sanjay Rajagopalan et al.
The Medical clinics of North America, 87(2), 441-457 (2003-04-16)
Based upon the results of the RALES trial and accumulating evidence about the role of aldosterone and aldosterone receptor antagonism in various disease states, the authors anticipate that aldosterone receptor antagonists will become standard therapy, along with ACE inhibitors and...
M K Agarwal et al.
Biochemical and biophysical research communications, 183(2), 405-410 (1992-03-16)
Lung cytosol from male, adrenalectomized rats was screened for the mineralocorticoid receptor (MCR) by a polyclonal antiserum raised in the rabbit against rat renal antigen. Western blot analysis revealed a single 98 kDa band, like the MCR purified biochemically. The...
Saaeha Rauz et al.
Investigative ophthalmology & visual science, 44(4), 1643-1651 (2003-03-27)
In peripheral sodium-transporting tissues, the serum- and glucocorticoid-regulated kinase (SGK) isoform-1 is an early corticosteroid target gene in the activation of epithelial sodium channels (ENaCs). Sodium transport across the human ocular nonpigmented and pigmented ciliary epithelial bilayer (NPE-PE) is essential...
A Souque et al.
The Journal of steroid biochemistry and molecular biology, 57(5-6), 315-321 (1996-03-01)
To investigate the role of sulfhydryl groups in the interaction of agonists and antagonists with the human mineralocorticoid receptor (hMR) the effect of methyl methanethiosulfonate (MMTS) on free and liganded-hMR was examined. hMR was expressed in insect cells (Sf9) using...
M Mirshahi et al.
Biochemical and biophysical research communications, 186(2), 1102-1107 (1992-07-31)
The multiplication of Chlamydomonas reinhardtii wild type cells can be arrested by the spirolactone RU 26752 and this is fully reversible by the natural mineralocorticoid aldosterone. Evidence is presented for a 52 kDa protein that possesses functional DNA and ligand...
M Mirshahi et al.
Biochemical medicine and metabolic biology, 47(2), 133-144 (1992-04-01)
We have obtained a polyclonal antiserum by immunizing fawn Burgundy rabbits with the mineralocorticoid receptor (MCR) purified biochemically from rat kidneys. High titers of anti-MCR activity were obtained in radioimmunoassays within 3 weeks and increased with a booster shot. In...
V B Singh et al.
European journal of pharmacology, 256(2), 177-184 (1994-04-21)
The rapidly reversible increase in cortical or midbrain tryptophan hydroxylase activity observed ex vivo after exposure of rats to 1-h sound stress was blocked by hypophysectomy, but not sham hypophysectomy, and restored by dexamethasone administration to the hypophysectomized animals (500...
P Penfornis et al.
American journal of physiology. Endocrinology and metabolism, 279(2), E386-E394 (2000-07-27)
By use of targeted oncogenesis, a brown adipocyte cell line was derived from a hibernoma of a transgenic mouse carrying the proximal promoter of the human mineralocorticoid receptor (MR) linked to the SV40 large T antigen. T37i cells remain capable...
E Köhler et al.
Journal of vascular research, 33(4), 315-326 (1996-07-01)
To investigate the hypothesis that aldosterone plays a role in the development of fibrosis, cultured fibroblasts from adult rat heart have been examined for their expression of aldosterone receptors and the effects of aldosterone on collagen synthesis. Binding assays with...
M Mirshahi et al.
International journal of toxicology, 21(3), 191-199 (2002-06-11)
Although the administration of adrenocortical hormones to pregnant rats provokes only limited effect on the growth and development of the fetus, the direct influence of these steroids on cultured embryos has never been studied. The disruption of cell signaling by...
M K Agarwal et al.
Neuroendocrinology, 58(5), 575-580 (1993-11-01)
The mineralocorticoid receptor (MCR) in the brain of adult male rats was analyzed with the aid of an antiserum generated by immunizing rabbits with this protein purified biochemically from rat kidney. In Western blots, the antibody recognized a single band...
G Lazar et al.
Biochimica et biophysica acta, 1033(1), 41-48 (1990-01-29)
Cardiac cytosol from adrenalectomized rats was radiolabelled with 10 nM tritiated RU 26752, R 5020 or aldosterone, to saturate the mineralocorticoid receptor (MCR) in the presence of 1 microM RU 38486 to block the glucocorticoid and progestin receptors. Free steroids...
T Hayashi et al.
The American journal of physiology, 261(1 Pt 1), C106-C114 (1991-07-01)
Increased levels of corticosteroids result in the development of hypertension in vivo. To investigate whether corticosteroids modulate calcium handling in vascular smooth muscle cells, we studied 45Ca2+ uptake and binding of [methyl-3H]PN 200-110, a potent dihydropyridine Ca2+ antagonist, in A7r5...

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