Showing 1-30 of 34 results for "

RES6007H-A7

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R A Romanov et al.
Rossiiskii fiziologicheskii zhurnal imeni I.M. Sechenova, 98(12), 1578-1586 (2013-03-07)
It is widely accepted that ATP secretion in diverse cells involves pannexin 1 (Panx1) that functions as an ATP-permeable channel. We analyzed the permeability of Panx1 channels heterologically expressed in HEK-293 cells to a variety of anions, including ATP. As...
Sebastian Marwitz et al.
Laboratory investigation; a journal of technical methods and pathology, 94(8), 927-933 (2014-06-17)
Alterations in the DNA methylome are characteristic for numerous diseases and a typical hallmark of cancer. Therefore, DNA methylation is currently under investigation in research labs and has also entered diagnostics. Recently, protocols like the BeadChip technology have become commercially...
Madeline Wong et al.
Biochemistry, 52(37), 6548-6558 (2013-09-03)
To date, little work has been conducted on the relationship between solute and buffer molecules and conformational exchange motion in enzymes. This study uses solution NMR to examine the effects of phosphate, sulfate, and acetate in comparison to MES- and...
Wen-Yih Jeng et al.
Acta crystallographica. Section D, Biological crystallography, 68(Pt 7), 829-838 (2012-07-04)
NkBgl, a β-glucosidase from Neotermes koshunensis, is a β-retaining glycosyl hydrolase family 1 enzyme that cleaves β-glucosidic linkages in disaccharide or glucose-substituted molecules. β-Glucosidases have been widely used in several applications. For example, mutagenesis of the attacking nucleophile in β-glucosidase...
Frauke Pedersen et al.
Respiratory medicine, 107(4), 587-595 (2013-01-15)
Induced sputum is a non-invasive sampling technique for the analysis of airway inflammation in various lung diseases and comprises valuable potential for the identification of biomarkers and therapeutic targets by molecular methods. In the context of biobanking with preservation of...
Rakesh K Pathak et al.
Analytical chemistry, 84(11), 5117-5123 (2012-05-04)
An in situ prepared Zn(2+) complex of triazole linked imino-thiophenyl conjugate of calix[4]arene, [ZnL], was demonstrated to be highly fluorescent in HEPES buffer solution. [ZnL] has been used as a chemo-sensing ensemble for the recognition of phosphates in general and...
A Akhlaghi et al.
Poultry science, 92(4), 874-881 (2013-03-09)
A hypothesis was tested that the in ovo injection of biological buffers may reinforce the buffering capacity of albumen, thereby withstanding the increase in albumen pH during storage and improving hatchability and chick quality in long-term stored eggs. Hatching eggs...
Jemimah Walker et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 100(4), 1134-1141 (2012-02-15)
Magnesium (Mg) and its alloys have been proposed as degradable replacements to commonly used orthopedic biomaterials such as titanium alloys and stainless steel. However, the corrosion of Mg in a physiological environment remains a difficult characteristic to accurately assess with...
Junbo Li et al.
Dalton transactions (Cambridge, England : 2003), 41(13), 3623-3626 (2012-02-24)
We developed a novel method for Cu(2+) detection based on the ion promoted oxidation reaction. Chemodosimeter L (weak fluorescence) can be oxidized into 3-benzothiazoly-7-N,N-diethylaminocoumarin (strong green fluorescence, coumarin 6) by Cu(2+) with high selectivity and sensitivity in HEPES (10 mM...
Nicole A Renner et al.
Journal of cellular physiology, 228(6), 1284-1294 (2012-11-17)
The foot processes of astrocytes cover over 60% of the surface of brain microvascular endothelial cells, regulating tight junction integrity. Retraction of astrocyte foot processes has been postulated to be a key mechanism in pathology. Therefore, movement of an astrocyte...
Anca G Delgado et al.
Microbial cell factories, 11, 128-128 (2012-09-15)
Buffering to achieve pH control is crucial for successful trichloroethene (TCE) anaerobic bioremediation. Bicarbonate (HCO3-) is the natural buffer in groundwater and the buffer of choice in the laboratory and at contaminated sites undergoing biological treatment with organohalide respiring microorganisms....
Peng Yang
Macromolecular bioscience, 12(8), 1053-1059 (2012-06-19)
When lysozyme is dissolved in a neutral HEPES buffer solution (pH = 7.4) with 0.001-0.050 M TCEP added, a fast phase transition process occurs and the resulting novel fiber-like hierarchical supramolecular assemblies made by primary spherical-particle aggregation can function as a "superglue"...
Hui Li et al.
Chemistry, an Asian journal, 6(9), 2320-2331 (2011-06-11)
A facile, template-free, and environmentally friendly hydrothermal strategy was explored for the controllable synthesis of α-Fe(2)O(3) nanostructures in HEPES solution (HEPES=2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid). The effects of experimental parameters including HEPES/FeCl(3) molar ratio, pH value, reaction temperature, and reaction time on the...
Hubert Gojzewski et al.
Scanning, 34(3), 159-169 (2011-09-29)
In this study, we deposited isolated magnetosomes from magnetotactic bacteria Magnetospirillum strain AMB-1 onto solid surfaces using spin coating (SC) and drop coating (DC) techniques. Four imaging techniques have been used to visualize the sample structure: scanning and transmission electron...
Teruo Shimmen
Journal of plant research, 125(6), 749-754 (2012-03-29)
In the previous paper, I reported that 3,5-dibromo-4-hydroxybenzonitrile (bromoxynil) depolarizes the plasma membrane by inhibiting the electrogenic proton pump and discussed that the inhibition is caused by cytosol acidification due to influx of protonated bromoxynil and following release of proton...
Rakesh Kumar Pathak et al.
The Analyst, 137(17), 4069-4075 (2012-07-24)
A highly fluorescent Zn(2+) complex of the triazole linked salicyl-imino-thiophenyl conjugate of calix[4]arene, [ZnL] has been demonstrated to be a chemo-sensing ensemble for the recognition of His and Cys among the naturally occurring amino acids in HEPES buffer milieu. The...
Darren Locke et al.
The Journal of general physiology, 138(3), 321-339 (2011-08-17)
The mechanisms of action of endogenous modulatory ligands of connexin channels are largely unknown. Previous work showed that protonated aminosulfonates (AS), notably taurine, directly and reversibly inhibit homomeric and heteromeric channels that contain Cx26, a widely distributed connexin, but not...
Qing Dai et al.
Analytical chemistry, 83(17), 6559-6564 (2011-08-02)
A ratiometric fluorescence sensor based on pyrene was designed for selective detection of heparin in HEPES (N-(2-hydroxyethyl)piperazine-N'-ethanesulfonic acid) buffer and serum sample. Pyrene and long-chain alkanes were linked through bisquaternary functionality in the sensor which could interact with heparin via...
Kirill Grushin et al.
Thrombosis and haemostasis, 113(4), 741-749 (2015-01-16)
Nanodiscs (ND) are lipid bilayer membrane patches held by amphiphilic scaffolding proteins (MSP) of ~10 nm in diameter. Nanodiscs have been developed as lipid nanoplatforms for structural and functional studies of membrane and membrane associated proteins. Their size and monodispersity...
Jesús Luis Yániz et al.
Microscopy research and technique, 75(4), 520-523 (2012-05-04)
This study was designed to evaluate the effect of various buffers on the fluorescence signal intensity of two fluorochromes (IP and CFDA) when used to assess the membrane integrity of ram sperm. Second ejaculates (18) from nine adult males were...
J R Eidet et al.
Experimental eye research, 94(1), 109-116 (2011-12-14)
The use of amniotic membrane (AM) represents one of the major developments in ocular surface reconstruction. However, in a study on patients with primary pterygium, transplantation of AM with ex vivo expanded human conjunctival epithelial cells (HCjE) promoted earlier epithelialization...
Heike Vogel et al.
Nucleosides, nucleotides & nucleic acids, 32(1), 17-27 (2013-01-31)
Rapid, template-directed ligation reactions between a phosphate-terminated oligonucleotide and an unphosphorylated reaction partner may be induced by cyanogen bromide (BrCN). Frequently, however, the reaction is low yielding, and even a large excess of the condensing agent can fail to induce...
Yuan Zhou et al.
Toxicology and applied pharmacology, 287(1), 1-8 (2015-05-20)
Microcystin (MC)-LR, a cyclic heptapeptide, is a potent reproductive system toxin. To understand the molecular mechanisms of MC-induced reproductive system cytotoxicity, we evaluated global changes of miRNA and mRNA expression in mouse Sertoli cells following MC-LR treatment. Our results revealed...
Matthew A Will et al.
Journal of assisted reproduction and genetics, 28(8), 711-724 (2011-05-27)
Minimizing environmental stress helps maintain cellular homeostasis and is a crucial component in optimizing embryo development in vitro and resulting ART success. One stressor of particular interest is pH. Biologic buffers, such as HEPES and MOPS, are valuable tools for...
Nicholas A Courtney et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(22), 7645-7656 (2014-05-30)
Metabotropic transmission typically occurs through the spillover activation of extrasynaptic receptors. This study examined the mechanisms underlying somatodendritic dopamine and noradrenaline transmission and found that the extent of spillover and pooling varied dramatically between these two transmitters. In the mouse...
Christian Giaume et al.
Methods in molecular biology (Clifton, N.J.), 814, 283-303 (2011-12-07)
A typical feature of astrocytes is their high level of connexin expression. These membrane proteins constitute the molecular basis of two types of channels: gap junction channels that allow direct cytoplasm-to-cytoplasm communication and hemichannels that provide a pathway for exchanges...
S Hugel et al.
British journal of pharmacology, 166(8), 2402-2416 (2012-03-29)
Artificial buffers such as HEPES are extensively used to control extracellular pH (pH(e) ) to investigate the effect of H(+) ions on GABA(A) receptor function. In neurones cultured from spinal cord dorsal horn (DH), dorsal root ganglia (DRG) and cerebellar...
James R Reed et al.
Toxicology and applied pharmacology, 289(2), 223-230 (2015-10-02)
Combustion processes generate different types of particulate matter (PM) that can have deleterious effects on the pulmonary and cardiovascular systems. Environmentally persistent free radicals (EPFRs) represent a type of particulate matter that is generated after combustion of environmental wastes in...
James R Reed et al.
Biochemical pharmacology, 95(2), 126-132 (2015-03-31)
Combustion processes generate particulate matter (PM) that can affect human health. The presence of redox-active metals and aromatic hydrocarbons in the post-combustion regions results in the formation of air-stable, environmentally persistent free radicals (EPFRs) on entrained particles. Exposure to EPFRs...
Tina Vognsen et al.
Biochemical and biophysical research communications, 420(1), 188-192 (2012-03-15)
The crystal structure of the NTF2-like domain of the Drosophila homolog of Ras GTPase SH3 Binding Protein (G3BP), Rasputin, was determined at 2.7Å resolution. The overall structure is highly similar to nuclear transport factor 2: It is a homodimer comprised...