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Yun Shu et al.
Lab on a chip, 13(5), 860-865 (2013-01-10)
Labeling of viruses can be used to reveal viral infection pathways and screen potential anti-viral drugs. Complex procedures, including virus cultivation, purification and labeling are involved in traditional virus labeling methods. And the manipulation of living virus brings risk to
Yu W Chu et al.
Chemical communications (Cambridge, England), 49(24), 2397-2399 (2013-01-19)
Described herein is a unique and inexpensive method that outperforms commercial methods that amplify the streptavidin-biotin recognition event. Amplification induced by streptavidin and biotinylated protein causes the formation of a large detectable polymer. This approach enjoys a 100-fold decrease in
Christina Puckert et al.
Acta biomaterialia, 106, 156-169 (2020-02-23)
Single Cell Force Spectroscopy was applied to measure the single cell de-adhesion between human neural stem cells (hNSC) and gelatin methacrylate (GelMA) hydrogel with varying modulus in the range equivalent to brain tissue. The cell de-adhesion force and energy were
Viviana I Risca et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(8), 2913-2918 (2012-02-07)
Mechanical cues affect many important biological processes in metazoan cells, such as migration, proliferation, and differentiation. Such cues are thought to be detected by specialized mechanosensing molecules linked to the cytoskeleton, an intracellular network of protein filaments that provide mechanical
Tasrina Munmun et al.
Chemical communications (Cambridge, England), 56(8), 1187-1190 (2020-01-11)
Trimethylamine N-oxide is found to be effective in regulating the interaction between microtubules and kinesins over a wide temperature range. The lifetime of the motility of microtubules on kinesins at high temperatures is prolonged using trimethylamine N-oxide. The activation energy
J Brooks Crickard et al.
Cell, 181(6), 1380-1394 (2020-06-06)
Homologous recombination (HR) helps maintain genome integrity, and HR defects give rise to disease, especially cancer. During HR, damaged DNA must be aligned with an undamaged template through a process referred to as the homology search. Despite decades of study
Daniel R Burnham et al.
Nature communications, 10(1), 2159-2159 (2019-05-16)
Accurate DNA replication is tightly regulated in eukaryotes to ensure genome stability during cell division and is performed by the multi-protein replisome. At the core an AAA+ hetero-hexameric complex, Mcm2-7, together with GINS and Cdc45 form the active replicative helicase
Douglas B Craig et al.
The protein journal, 32(2), 81-88 (2013-01-18)
Single molecule assays were performed on streptavidin-β-galactosidase using a capillary electrophoresis-based protocol in order to assess the suitability of single molecule β-galactosidase assays for adaptation to the detection of single copies of target DNA. The conjugate was found to have
A rapid streptavidin-capture ELISA specific for the detection of antibodies to feline foamy virus.
Winkler IG et al
Journal of Immunological Methods, 207(1), 69-77 (1997)
Sjoerd Rijpkema et al.
Biologicals : journal of the International Association of Biological Standardization, 56, 29-38 (2018-09-12)
Vi capsular polysaccharide (Vi) conjugate vaccines, which can prevent typhoid in infants and young children, are being developed. Comparative immunogenicity studies are facilitated by an International Standard (IS) for human anti-Vi IgG. 16/138, a pool of sera from volunteers which
Harry Towbin et al.
Nucleic acids research, 41(3), e47-e47 (2012-12-12)
We describe a new, broadly applicable methodology for screening in parallel interactions of RNA-binding proteins (RBPs) with large numbers of microRNA (miRNA) precursors and for determining their affinities in native form in the presence of cellular factors. The assays aim
Hazal B Kose et al.
Cell reports, 26(8), 2113-2125 (2019-02-21)
Progression of DNA replication depends on the ability of the replisome complex to overcome nucleoprotein barriers. During eukaryotic replication, the CMG helicase translocates along the leading-strand template and unwinds the DNA double helix. While proteins bound to the leading-strand template
Peter Rigsby et al.
Biologicals : journal of the International Association of Biological Standardization, 66, 21-29 (2020-06-24)
Typhoid vaccines based on protein-conjugated capsular Vi polysaccharide (TCVs) prevent typhoid in infants and young children. Analysis of the serum anti-Vi IgG response following immunisation against typhoid confirms the immunogenicity of TCVs and forms an important part of the pathway
Yuanchang Zhao et al.
iScience, 9, 502-512 (2018-11-26)
Rapid cell migration requires efficient rear de-adhesion. It remains undetermined whether cells mechanically detach or biochemically disassemble integrin-mediated rear adhesion sites in highly motile cells such as keratocytes. Using molecular tension sensor, we calibrated and mapped integrin tension in migrating
Anthony W Partridge et al.
Molecules (Basel, Switzerland), 24(12) (2019-06-23)
Stapled α-helical peptides represent an emerging superclass of macrocyclic molecules with drug-like properties, including high-affinity target binding, protease resistance, and membrane permeability. As a model system for probing the chemical space available for optimizing these properties, we focused on dual
Inés Beekers et al.
Journal of controlled release : official journal of the Controlled Release Society, 322, 426-438 (2020-04-05)
Ultrasound insonification of microbubbles can locally increase vascular permeability to enhance drug delivery. To control and optimize the therapeutic potential, we need to better understand the underlying biological mechanisms of the drug delivery pathways. The aim of this in vitro
Structural origins of high-affinity biotin binding to streptavidin.
Weber PC et al
Science, 243(4887), 85-88 (1989)
Characterization and crystallization of core streptavidin.
Pahler A et al
The Journal of Biological Chemistry, 262(29), 13933-13937 (1987)
Maria Hammond et al.
Prion, 8(3), 261-265 (2014-12-09)
The DNA assisted solid-phase proximity ligation assay (SP-PLA) provides a unique opportunity to specifically detect prion protein (PrP) aggregates by investigating the collocation of 3 or more copies of the specific protein. We have developed an SP-PLA that can detect
Noa Furth et al.
medRxiv : the preprint server for health sciences (2021-06-03)
The COVID-19 pandemic raises the need for diverse diagnostic approaches to rapidly detect different stages of viral infection. The flexible and quantitative nature of single-molecule imaging technology renders it optimal for development of new diagnostic tools. Here we present a
Ross A Robinson et al.
Cell, 184(8), 2103-2120 (2021-03-20)
During cell migration or differentiation, cell surface receptors are simultaneously exposed to different ligands. However, it is often unclear how these extracellular signals are integrated. Neogenin (NEO1) acts as an attractive guidance receptor when the Netrin-1 (NET1) ligand binds, but
Paul H Bessette et al.
Protein engineering, design & selection : PEDS, 17(10), 731-739 (2004-11-09)
A robust bacterial display methodology was developed that allows the rapid isolation of peptides that bind to arbitrarily selected targets with high affinity. To demonstrate the utility of this approach, a large library (5 x 10(10) clones) was constructed composed
Iris Dahan et al.
The Journal of biological chemistry, 277(10), 8421-8432 (2001-12-26)
The superoxide-generating NADPH oxidase complex of phagocytes consists of a membranal heterodimeric flavocytochrome (cytochrome b(559)), composed of gp91(phox) and p22(phox) subunits, and four cytosolic proteins, p47(phox), p67(phox), p40(phox), and the small GTPase Rac (1 or 2). All redox stations involved
Kai Wu et al.
ACS applied materials & interfaces, 11(26), 22979-22986 (2019-06-30)
Magnetic nanoparticles (MNPs) have been extensively used as contrasts and tracers for bioimaging, heating sources for tumor therapy, carriers for controlled drug delivery, and labels for magnetic immunoassays. Here, we describe a MNP Brownian relaxation dynamics-based magnetic particle spectroscopy (MPS)
Ryota Sakata et al.
Cell reports, 35(4), 108999-108999 (2021-04-29)
The ring-shaped cohesin complex topologically binds to DNA to establish sister chromatid cohesion. This topological binding creates a structural obstacle to genome-wide chromosomal events, such as replication. Here, we examine how conformational changes in cohesin circumvent being an obstacle in
Rahim Esfandyarpour et al.
Biomedical microdevices, 18(1), 7-7 (2016-01-19)
The improvements in our ability to sequence and genotype DNA have opened up numerous avenues in the understanding of human biology and medicine with various applications, especially in medical diagnostics. But the realization of a label free, real time, high-throughput
Camille Hallez et al.
Nature microbiology, 4(7), 1196-1207 (2019-04-03)
Numerous human APOBEC3 cytidine deaminases have proven to be, inter alia, host cell restriction factors for retroviruses and hepadnaviruses. Although they can bind to genomic RNA and become encapsidated, they are only catalytically active on single-stranded DNA. As there are
Giuseppe Arena et al.
Molecular cell, 69(4), 594-609 (2018-02-18)
Accumulating evidence indicates that the MDM2 oncoprotein promotes tumorigenesis beyond its canonical negative effects on the p53 tumor suppressor, but these p53-independent functions remain poorly understood. Here, we show that a fraction of endogenous MDM2 is actively imported in mitochondria
Noa Furth et al.
PloS one, 16(7), e0255096-e0255096 (2021-07-27)
The COVID-19 pandemic raises the need for diverse diagnostic approaches to rapidly detect different stages of viral infection. The flexible and quantitative nature of single-molecule imaging technology renders it optimal for development of new diagnostic tools. Here we present a
Mapping of functional domains in the p22(phox) subunit of flavocytochrome b(559) participating in the assembly of the NADPH oxidase complex by "peptide walking".
Dahan I et al
The Journal of Biological Chemistry, 277(10), 8421-8432 (2002)
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