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Dyes for in vitro detection of nucleic acids and proteins.
BioProbes, 20, 12-13 (1994)
Shear-resistant hydrogels to control permeability of porous tubular scaffolds in vascular tissue engineering
Tresoldi C, et al.
Materials Science and Engineering, C, 105, 110035-110035 (2019)
The in vitro effect of nonylphenol, propranolol, and diethylstilbestrol on quality parameters and oxidative stress in sterlet (Acipenser ruthenus) spermatozoa
Shaliutina O, et al.
Toxicology in vitro, 43, 9-15 (2017)
Fintan K T Stanley et al.
Nucleic acids research, 48(19), e111-e111 (2020-10-04)
Ionizing radiation (IR) is environmentally prevalent and, depending on dose and linear energy transfer (LET), can elicit serious health effects by damaging DNA. Relative to low LET photon radiation (X-rays, gamma rays), higher LET particle radiation produces more disease causing...
Deborah Traversi et al.
Scientific reports, 10(1), 17566-17566 (2020-10-18)
Type 1 diabetes (T1D) is a common autoimmune disease that is characterized by insufficient insulin production. The onset of T1D is the result of gene-environment interactions. Sociodemographic and behavioural factors may contribute to T1D, and the gut microbiota is proposed...
Sebastian Baumgarten et al.
Nature microbiology, 4(12), 2246-2259 (2019-08-07)
Malaria pathogenesis results from the asexual replication of Plasmodium falciparum within human red blood cells, which relies on a precisely timed cascade of gene expression over a 48-h life cycle. Although substantial post-transcriptional regulation of this hardwired program has been...
Huiyuan Zhang et al.
Acta histochemica, 122(5), 151571-151571 (2020-07-06)
Emerging evidence has unraveled the important implications of microRNAs (miRNAs/miRs) in intracerebral hemorrhage (ICH). The aim of the present study was to assess the possible regulatory role of miR-26a-5p in ICH both in vivo and in vitro. ICH model of...
Philip Webster et al.
Nature communications, 9(1), 2649-2649 (2018-07-10)
Determining whether recurrent but rare cancer mutations are bona fide driver mutations remains a bottleneck in cancer research. Here we present the most comprehensive analysis of murine leukemia virus-driven lymphomagenesis produced to date, sequencing 700,000 mutations from >500 malignancies collected...
Daniel Clemente de Moraes et al.
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 51(3), 1051-1060 (2020-03-12)
The aim of this study was to evaluate the ability of lapachones in disrupting the fungal multidrug resistance (MDR) phenotype, using a model of study which an azole-resistant Saccharomyces cerevisiae mutant strain that overexpresses the ATP-binding cassette (ABC) transporter Pdr5p....
Jina Heo et al.
Scientific reports, 10(1), 10647-10647 (2020-07-02)
Algae-bacteria interaction is one of the main factors underlying the formation of harmful algal blooms (HABs). The aim of this study was to develop a genome-wide high-throughput screening method to identify HAB-influenced specific interactive bacterial metabolites using a comprehensive collection...
Sergey V Prykhozhij et al.
Disease models & mechanisms, 10(6), 811-822 (2017-03-11)
Clustered regularly interspaced palindromic repeats (CRISPR)/Cas-based adaptive immunity against pathogens in bacteria has been adapted for genome editing and applied in zebrafish (
Gautam Vishwakarma et al.
PloS one, 14(11), e0224572-e0224572 (2019-11-07)
Sequence tagged microsatellite site (STMS) are useful PCR based DNA markers. Wide genome coverage, high polymorphic index and co-dominant nature make STMS a preferred choice for marker assisted selection (MAS), genetic diversity analysis, linkage mapping, seed genetic purity analysis etc....
I Ortiz et al.
Animal reproduction science, 187, 74-78 (2017-10-19)
The aim of this study was to compare sperm DNA fragmentation of frozen-thawed epididymal sperm of dogs using the SCSA (Sperm Chromatin Structure Assay) and SCDt (Sperm Chromatin Dispersion test). For this purpose, epididymis from neutered dogs were minced and...
Loop mediated isothermal amplification of 5.8S rDNA for specific
detection of Tritrichomonas foetus
Jorge Oyhenart
Veterinary Parasitology, 193 (2013)
Geng Chen et al.
Gastroenterology research and practice, 2020, 1343969-1343969 (2020-05-10)
This study was aimed at investigating the effect of baicalin on experimental cholesterol gallstones in mice. The mouse gallstone model was induced by feeding with a lithogenic diet, and cholesterol stones were found in the gallbladder. The lithogenic diet caused...
Kerstin Göpfrich et al.
ACS synthetic biology, 8(5), 937-947 (2019-05-03)
Here, we introduce a one-pot method for the bottom-up assembly of complex single- and multicompartment synthetic cells. Cellular components are enclosed within giant unilamellar vesicles (GUVs), produced at the milliliter scale directly from small unilamellar vesicles (SUVs) or proteoliposomes with...
Jie Xiong et al.
International journal of molecular medicine, 46(3), 1003-1012 (2020-06-26)
Alcoholic liver disease greatly affects human health. Previous studies have identified that microRNAs (miRNAs) are associated with the pathogenesis of alcoholic liver fibrosis (ALF). Therefore, the present study explored the regulatory mechanism of miR‑148a‑3p in ALF. An ALF model was established in...
P A Morin et al.
BioTechniques, 19(2), 223-228 (1995-08-01)
A method for resolving and visualizing genotypes for simple sequence repeat loci on short (20 cm) polyacrylamide gels is described. This method makes use of a modified vertical electrophoresis cell to allow rapid electrophoresis without variation in migration rates due...
Raz Bar-Ziv et al.
Cell reports, 30(12), 3989-3995 (2020-03-27)
Genome replication perturbs the DNA regulatory environment by displacing DNA-bound proteins, replacing nucleosomes, and introducing dosage imbalance between regions replicating at different S-phase stages. Recently, we showed that these effects are integrated to maintain transcription homeostasis: replicated genes increase in...
Evelien Eenjes et al.
Scientific reports, 8(1), 7349-7349 (2018-05-11)
Air-liquid interface (ALI) cultures of mouse tracheal epithelial cells (MTEC) are a well-established model to study airway epithelial cells, but current methods require large numbers of animals which is unwanted in view of the 3R principle and introduces variation. Moreover...
Gayathri Kanika et al.
Journal of biochemical and molecular toxicology, 29(8), 349-359 (2015-03-17)
Several reports indicated that histone deacetylases (HDACs) play a crucial role in inflammation and fibrogenesis. Sodium butyrate (SB) is a short-chain fatty acid having HDAC inhibition potential. The present study aimed to evaluate the protective effect of SB against L-arginine...
Zhi-Ping Zhong et al.
Microbiome, 9(1), 160-160 (2021-07-21)
Glacier ice archives information, including microbiology, that helps reveal paleoclimate histories and predict future climate change. Though glacier-ice microbes are studied using culture or amplicon approaches, more challenging metagenomic approaches, which provide access to functional, genome-resolved information and viruses, are...
Biomed. Prod., 19, 68-68 (1994)
Yien Xiang et al.
Journal of physiology and biochemistry, 76(1), 123-134 (2020-01-30)
MiR-23a-3p has been shown to promote liver cancer cell growth and metastasis and regulate that of chemosensitivity. Protocadherin17 (PCDH17) is a tumor suppressor gene and plays an essential part in cell cycle of hepatocellular carcinoma (HCC). This study aimed at...
Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications
Zipper H, et al.
Nucleic Acids Research, 32(12), e103-e103 (2004)
Ruili Wang et al.
Genes to cells : devoted to molecular & cellular mechanisms, 25(6), 364-374 (2020-03-12)
Microglial inflammation is identified as a key process associated with Parkinson's disease (PD) pathogenesis. Our previous study showed that miR-29c-3p (miR-29c) exhibited anti-inflammatory properties in PD animal and neuronal models. However, the specific role and regulatory mechanism of miR-29c played...
Rebecca H Herbst et al.
BMC biology, 15(1), 38-38 (2017-05-13)
The merging of genomes in inter-specific hybrids can result in novel phenotypes, including increased growth rate and biomass yield, a phenomenon known as heterosis. Heterosis is typically viewed as the opposite of hybrid incompatibility. In this view, the superior performance...
Ali Moeini-Zanjani et al.
BMC research notes, 13(1), 533-533 (2020-11-15)
Rapid, reliable, and affordable detection of Brucella species via the molecular methods remains a challenge. In recent years, loop-mediated isothermal amplification (LAMP) is a functional nucleic acid amplification technique offering a substitute to polymerase chain reaction (PCR). So, we compared...
Claudia Tresoldi et al.
Materials science & engineering. C, Materials for biological applications, 105, 110035-110035 (2019-09-25)
Aiming to perfuse porous tubular scaffolds for vascular tissue engineering (VTE) with controlled flow rate, prevention of leakage through the scaffold lumen is required. A gel coating made of 8% w/v alginate and 6% w/v gelatin functionalized with fibronectin was...
Charles J Woodrow et al.
Journal of clinical microbiology, 53(10), 3296-3303 (2015-08-14)
Flow cytometry is an objective method for conducting in vitro antimalarial sensitivity assays with increasing potential for application in field sites. We examined in vitro susceptibility to seven anti-malarial drugs for 40 fresh P. falciparum field isolates via a flow...
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