Showing 1-30 of 79 results for "SML1046"
Hyogeun Shin et al.
Nature communications, 12(1), 492-492 (2021-01-23)
Investigation of neural circuit dynamics is crucial for deciphering the functional connections among regions of the brain and understanding the mechanism of brain dysfunction. Despite the advancements of neural circuit models in vitro, technologies for both precisely monitoring and modulating...
Cheuk Yiu Tenny Chung et al.
Biomedicines, 8(10) (2020-10-15)
BRISC and BRCA1-A complex member 2 (Babam2) plays an essential role in promoting cell cycle progression and preventing cellular senescence. Babam2-deficient fibroblasts show proliferation defect and premature senescence compared with their wild-type (WT) counterpart. Pluripotent mouse embryonic stem cells (mESCs)...
Chunbo Yang et al.
Nature communications, 11(1), 3812-3812 (2020-08-01)
Vascular endothelial cell (EC) dysfunction plays a key role in diabetic complications. This study discovers significant upregulation of Quaking-7 (QKI-7) in iPS cell-derived ECs when exposed to hyperglycemia, and in human iPS-ECs from diabetic patients. QKI-7 is also highly expressed...
Generation of Human-Induced Pluripotent Stem Cells in the Absence of Exogenous Sox2
Li W
Stem Cells, 27, 2992-3000 (2009)
Adriana Buskin et al.
Nature communications, 9(1), 4234-4234 (2018-10-14)
Mutations in pre-mRNA processing factors (PRPFs) cause autosomal-dominant retinitis pigmentosa (RP), but it is unclear why mutations in ubiquitously expressed genes cause non-syndromic retinal disease. Here, we generate transcriptome profiles from RP11 (PRPF31-mutated) patient-derived retinal organoids and retinal pigment epithelium...
Xiao-Jun Li et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(36), 22225-22236 (2020-08-23)
Mechano-sensory hair cells within the inner ear cochlea are essential for the detection of sound. In mammals, cochlear hair cells are only produced during development and their loss, due to disease or trauma, is a leading cause of deafness. In...
Cheryl Stork et al.
Nucleic acids research, 47(3), 1532-1543 (2018-11-30)
X-inactive-specific transcript (Xist) is a long noncoding RNA (lncRNA) essential for inactivating one of the two X chromosomes in mammalian females. Random X chromosome inactivation is mediated by Xist RNA expressed from the inactive X chromosome. We found that Xist...
The roles of Notch3 on the cell proliferation and apoptosis induced by CHIR99021 in NSCLC cell lines: a functional link between Wnt and Notch signaling pathways
Li C, et al.
PLoS ONE, 8(12), e84659-e84659 (2013)
Maria Georgiou et al.
PloS one, 15(6), e0233860-e0233860 (2020-06-02)
The generation of laminated and light responsive retinal organoids from induced pluripotent stem cells (iPSCs) provides a powerful tool for the study of retinal diseases and drug discovery and a robust platform for cell-based therapies. The aim of this study...
L V Albrecht et al.
STAR protocols, 1(3), 100132-100132 (2020-12-31)
Lysosomes are the catabolic center of the cell. Limitations of many lysosomal tracers include low specificity and lack of reliable physiological readouts for changes in growth factor-regulated lysosomal activity. The imaging-based protocols described here provide insights at the cellular level...
Le Tran Phuc Khoa et al.
Cell stem cell, 27(3), 441-458 (2020-07-02)
Self-renewing embryonic stem cells (ESCs) respond to environmental cues by exiting pluripotency or entering a quiescent state. The molecular basis underlying this fate choice remains unclear. Here, we show that histone acetyltransferase MOF plays a critical role in this process...
Jerzy A Woznicki et al.
Cell death & disease, 11(1), 68-68 (2020-01-29)
Proteins of the BCL-2 family are evolutionarily conserved modulators of apoptosis that function as sensors of cellular integrity. Over the past three decades multiple BCL-2 family members have been identified, many of which are now fully incorporated into regulatory networks...
CHIR99021 promotes self-renewal of mouse embryonic stem cells by modulation of protein-encoding gene and long intergenic non-coding RNA expression
Wu Y, et al.
Experimental Cell Research, 319(17), 2684-2699 (2013)
E Driehuis et al.
PloS one, 15(5), e0231588-e0231588 (2020-05-19)
We have recently established a protocol to grow wildtype human oral mucosa organoids. These three-dimensional structures can be maintained in culture long-term, do not require immortalization, and recapitulate the multilayered composition of the epithelial lining of the oral mucosa. Here...
Tzu-Cheng Sung et al.
Biomaterials, 253, 120060-120060 (2020-05-26)
The current differentiation process of human pluripotent stem cells (hPSCs) into cardiomyocytes to enhance the purity of hPSC-derived cardiomyocytes requires some purification processes, which are laborious processes. We developed cell sorting plates, which are prepared from coating thermoresponsive poly(N-isopropylacrylamide) and...
Jian Hui Low et al.
Cell stem cell, 25(3), 373-387 (2019-07-16)
Human pluripotent stem cell-derived kidney organoids recapitulate developmental processes and tissue architecture, but intrinsic limitations, such as lack of vasculature and functionality, have greatly hampered their application. Here we establish a versatile protocol for generating vascularized three-dimensional (3D) kidney organoids....
Dong Li et al.
Cell cycle (Georgetown, Tex.), 16(11), 1070-1084 (2017-04-21)
Previous research has shown that a subpopulation of cells within cultured human dermal fibroblasts, termed multilineage-differentiating stress enduring (Muse) cells, are preferentially reprogrammed into induced pluripotent stem cells. However, controversy exists over whether these cells are the only cells capable...
The Roles of Notch3 on the Cell Proliferation and Apoptosis Induced by CHIR99021 in NSCLC Cell Lines: A Functional Link between Wnt and Notch Signaling Pathways
Li C
PLoS ONE, 8 (2013)
Co-incident insertion enables high efficiency genome engineering in mouse embryonic stem cells
Shy BR
Nucleic Acids Research, 44, 7997-8010 (2016)
Daniel Zingg et al.
Cancer cell, 34(1), 69-84 (2018-07-17)
Human melanomas frequently harbor amplifications of EZH2. However, the contribution of EZH2 to melanoma formation has remained elusive. Taking advantage of murine melanoma models, we show that EZH2 drives tumorigenesis from benign BrafV600E- or NrasQ61K-expressing melanocytes by silencing of genes...
Susanne C van den Brink et al.
Nature, 582(7812), 405-409 (2020-02-23)
Gastruloids are three-dimensional aggregates of embryonic stem cells that display key features of mammalian development after implantation, including germ-layer specification and axial organization1-3. To date, the expression pattern of only a small number of genes in gastruloids has been explored...
CHIR99021 promotes self-renewal of mouse embryonic
stem cells by modulation of protein-encoding gene and
long intergenic non-coding RNA expression
Wu Y
Experimental Cell Research, 319, 2684-2699 (2013)
Generation of Urine Cell-Derived Non-integrative Human iPSCs and iNSCs: A Step-by-Step Optimized Protocol
Cheng L
Frontiers in Molecular Neuroscience (2017)
Molecular Diversity of Midbrain Development in Mouse, Human, and Stem Cells
La Manno G
Cell, 167, 566-580 (2016)
Xin Xie et al.
PLoS genetics, 14(12), e1007846-e1007846 (2018-12-18)
During neuronal development, β-actin serves an important role in growth cone mediated axon guidance. Consistent with this notion, in vivo ablation of the β-actin gene leads to abnormalities in the nervous system. However, whether β-actin is involved in the regulation...
Marta Vilà González et al.
Diabetes, 69(10), 2170-2185 (2020-08-17)
Diabetic macular edema (DME) remains a leading cause of vision loss worldwide. DME is commonly treated with intravitreal injections of vascular endothelial growth factor (VEGF)-neutralizing antibodies. VEGF inhibitors (anti-VEGFs) are effective, but not all patients fully respond to them. Given...
Yajing Gao et al.
Gastroenterology, 158(5), 1389-1401 (2020-01-14)
In addition to the Notch and Wnt signaling pathways, energy metabolism also regulates intestinal stem cell (ISC) function. Tumor suppressor and kinase STK11 (also called LKB1) regulates stem cells and cell metabolism. We investigated whether loss of LKB1 alters ISC...
Yun Ha Hur et al.
Developmental cell, 56(3), 277-291 (2020-12-16)
It is critical that epiblast cells within blastocyst-stage embryos receive the necessary regulatory cues to remain pluripotent until the appropriate time when they are stimulated to undergo differentiation, ultimately to give rise to an entire organism. Here, we show that...

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