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Enzymatic Method for Determining Glucose and Sucrose (Glucose and Sucrose Assay)
Assay protocol for the colorimetric/fluorometric detection of glucose and sucrose in biological samples using the glucose and sucrose assay kit.
Low-in-Nanoparticulate-Impurities Sucrose for Biopharmaceutical Formulations
Nanoparticulate impurities (NPIs) in pharmaceutical-grade sucrose reduce the stability of final protein formulations. Applying a purification process results in a low NPI sucrose, thus mitigating risk during formulation development.
Density Gradient
BioUltra Reagents Density Gradients.To meet highest demands for quality, Sigma-Aldrich offers a wide range of BioUltra Reagents for density gradient centrifugation
Enzymatic Assay of Invertase
Enzymatic Assay of Invertase
How to Make and Use Percoll Gradients
How to use percoll to prepare a gradient
How to make and use gradients of Percoll
In order to use Percoll to prepare a gradient, the osmolality of Percoll (undiluted) must first be adjusted with saline or cell culture medium to make Percoll isotonic with physiological salt solutions.
How to Fractionate and Analyze Gradients of Percoll
Density Marker Beads are dyed derivatives of Sephadex™. There are ten color-coded bead types, each with a specific density. They have been specifically formulated for use in Percoll gradients and will not work with other media. Using Density Marker Beads
Understanding Sweet Taste Perception
The powerful effect of the pleasurable sensation arising from sweet tasting foods has led to consumer demand and large-scale production of the caloric sweetening agents sucrose and fructose.
Enzymatic Food Analysis
Enzymatic methods for food analysis are highly specific and offer considerable time and cost savings over other methods, especially from the sample preparation standpoint. We offer a wide variety of convenient kits and reagents for rapid and reliable enzymatic food
Preparation of Antibody Sensitized Sheep Erythrocytes
Protocol for the preparation of Antibody Sensitized Sheep Erythrocytes. Includes product numbers and links.
Monoclonal Antibody Manufacturing
Monoclonal antibody manufacturing is a highly templated approach used to produce mAb-based immunotherapies. Robust, scalable process solutions are required at every step to ensure high therapeutic concentration and process safety, while meeting speed-to-market and cost containment concerns.
Cell Separation Media
Explore our complete line of popular density gradient media for the separation or extraction of leukocytes, viruses, DNA, RNA, organelles and other research and clinical applications.
Centrifugation Separations
Differential centrifugation and density gradient centrifugation are two types of centrifugal techniques for separating particles.CsCl gradient centrifugation, or Caesium chloride centrifugation is used to make solutions for the separation of RNA from DNA by density gradient centrifugation.
Monosaccharide Biosynthesis
Learn about monosaccharide biosynthesis and the metabolism of monosaccharides. A unit of a carbohydrate and the simplest form of a sugar, a monosaccharide cannot be hydrolyzed into a simpler compound.
Murashige and Skoog Plant Cell Culture Media Formulations
Basal Salts Mixtures, Media including Organics
Ficoll® 400 for Gradient Centrifugation
Ficoll 400® is a highly branched polymer formed by the copolymerization of sucrose and epichlorohydrin. Ficoll 400® is completely non-ionic. Because of the abundance of hydroxyl groups, Ficoll 400® is very hydrophilic and extremely water-soluble.
Protocol Guide: Exosome Labeling Using PKH Lipophilic Membrane Dyes
PKH dyes have been successfully used to label exosomes and extracellular vesicles for in vitro and in vivo tracking experiments. The exosome labeling protocol below details steps for reliable fluorescent labeling of exosomes using PKH dyes for microvesicle tracking experiments.
Protein Stabilizers
Our high-quality protein stabilizers provide the control you need for successful biopharmaceutical formulations and efficient processing, backed by comprehensive Emprove® documentation to help minimize regulatory and quality-associated risks.
Carbohydrate Standards
Analytical standards and certified reference materials/pharmaceutical reference standards of different carbohydrates (monosaccharides, disaccharides, oligosaccharides, and polysaccharides) for analytical testing in pharmaceutical and food and beverage industries.
The Fight to Prevent Food Fraud
Increasing needs to test the integrity or authenticity of food and beverages as food fraud scandals are on the rise. We have portfolios that provide reliable results when performing analyses on the authenticity of food.
Centrifugation Basics
Centrifugation enables the separation of particles by sedimentation. Learn how to separate particles using a centrifuge and how to use Stokes' law to calculate the velocity of sedimentation.
Media Preparation – Plant Tissue Culture Protocol
Preparation from Packaged Powder, Preparation from Basal Salt Solutions, Banana Powder Preparation and Use, Coconut Water Preparation and Use
Vibrio Detection
Vibrios are motile, curved or comma-shaped bacilli and have a single polar flagella with sheet proteins. They are often found in open water, freshwater and saltwater.
Chitosan-based Biomaterials
Chitin, a natural polysaccharide, is the second most abundant natural biopolymer in the world, after cellulose.
Nuclear Protein Extraction Without the Use of Detergent
A protocol for the preparation of nuclear proteins without the use of a detergent. Detergents can interfer with the labeling efficiency of the extracted proteins.
Extracellular Vesicle Preparation
Extracellular Vesicle Preparation learning center. Learn how to prepare Extracellular Vesicles Preparation, Amicon Ultra filters and new techniques for streamlined analysis of your EV preparations.
Microscopic Examination of Samples
This protocol covers 3 modes for the microscopic examination of cell samples.
Differentiation and Identification Media for Salmonella
Salmonella contamination is the second leading cause of food-borne illness worldwide. Controlling outbreaks of Salmonella is an important task for food regulators, restaurants and the food industry in general. The Salmonella family includes over 2,300 serotypes of bacteria, but two types
Ion Chromatography for Sugar Testing in Food and Environmental Analysis
In this article we demonstrate how well ion chromatography is suited as an analytical technique for sugar analysis e.g. in apple juice, milk, and dust samples.
Nuclear Protein Extraction Without the Use of Detergent
Detergents can interfere with the labeling efficiency of the extracted proteins. Therefore, a procedure that does not include the use of detergents should be used for the preparation of nuclear proteins.
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