Showing 1-30 of 34 results for "

T0665

"
Jie Zhou et al.
Cell stem cell, 24(2), 285-298 (2019-01-15)
The generation of hematopoietic stem cells (HSCs) from embryonic endothelial precursors and pre-HSCs is precisely regulated by signaling pathways and transcription factors. Nevertheless, regulatory roles of non-coding RNAs remain unknown. Taking advantage of our ability to capture rare pre-HSCs and HSCs...
Gaurav Kaushik et al.
Advanced healthcare materials, 8(2), e1801186-e1801186 (2018-12-20)
Vascularization is an important strategy to overcome diffusion limits and enable the formation of complex, physiologically relevant engineered tissues and organoids. Self-assembly is a technique to generate in vitro vascular networks, but engineering the necessary network morphology and function remains...
Stefano Menghini et al.
International journal of molecular sciences, 22(2) (2021-01-10)
Interest has grown in harnessing biological agents for cancer treatment as dynamic vectors with enhanced tumor targeting. While bacterial traits such as proliferation in tumors, modulation of an immune response, and local secretion of toxins have been well studied, less...
Hilal Varinli et al.
Journal of lipid research, 56(11), 2206-2216 (2015-09-04)
Lipid droplets (LDs) are the main storage organelles for triglycerides. Elucidation of lipid accumulation mechanisms and metabolism are essential to understand obesity and associated diseases. Adipogenesis has been well studied in murine 3T3-L1 and human Simpson-Golabi-Behmel syndrome (SGBS) preadipocyte cell...
Constancio Gonzalez-Obeso et al.
Acta biomaterialia, 88, 241-250 (2019-02-23)
The development of mucoadhesive materials is of great interest and is also a major challenge. Being adsorption sites, mucosae are suitable targets for drug delivery, but as defensive barriers they are complex biological surfaces to interact with, mainly due to...
Giorgia Salvagiotto et al.
PloS one, 6(3), e17829-e17829 (2011-03-30)
Human ESC and iPSC are an attractive source of cells of high quantity and purity to be used to elucidate early human development processes, for drug discovery, and in clinical cell therapy applications. To efficiently differentiate pluripotent cells into a...
Maira Di Tano et al.
Nature communications, 11(1), 2332-2332 (2020-05-13)
Fasting-mimicking diets delay tumor progression and sensitize a wide range of tumors to chemotherapy, but their therapeutic potential in combination with non-cytotoxic compounds is poorly understood. Here we show that vitamin C anticancer activity is limited by the up-regulation of...
Matthildi Valianou et al.
Cell cycle (Georgetown, Tex.), 14(3), 399-407 (2015-01-08)
The mechanistic target of rapamycin complex 1 (mTORC1) increases translation, cell size and angiogenesis, and inhibits autophagy. mTORC1 is negatively regulated by hamartin and tuberin, the protein products of the tumor suppressors TSC1 and TSC2 that are mutated in Tuberous...
James W Foster et al.
Scientific reports, 7, 41286-41286 (2017-01-28)
The cornea is the transparent outermost surface of the eye, consisting of a stratified epithelium, a collagenous stroma and an innermost single-cell layered endothelium and providing 2/3 of the refractive power of the eye. Multiple diseases of the cornea arise...
Senthil Raja Jayapal et al.
Cell cycle (Georgetown, Tex.), 15(22), 3070-3081 (2016-09-23)
Cyclin A2 is an essential gene for development and in haematopoietic stem cells and therefore its functions in definitive erythropoiesis have not been investigated. We have ablated cyclin A2 in committed erythroid progenitors in vivo using erythropoietin receptor promoter-driven Cre...
Xiaoxing Liang et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 135, 111015-111015 (2019-12-10)
Bisphenol A (BPA) and its derivatives, including bisphenols S (BPS), F (BPF), E (BPE), B (BPB), Z (BPZ), and AF (BPAF), are widely used in consumer products. Moreover, they are typically detected in the environment, food, and humans. Previous studies...
Kun Wang et al.
Cell, 180(5), 941-955 (2020-02-29)
The pyroptosis execution protein GSDMD is cleaved by inflammasome-activated caspase-1 and LPS-activated caspase-11/4/5. The cleavage unmasks the pore-forming domain from GSDMD-C-terminal domain. How the caspases recognize GSDMD and its connection with caspase activation are unknown. Here, we show site-specific caspase-4/11...
Xiaoxing Liang et al.
Journal of hazardous materials, 407, 124387-124387 (2020-11-12)
The widely used chemical bisphenol A (BPA) has been associated with several health effects. In recent years, many derivatives were developed to replace BPA although without thorough toxicological evaluation. Here, we employed a human embryoid body (EB)-based in vitro global...
Jugal Saharia et al.
Electrophoresis, 41(7-8), 630-637 (2019-11-12)
In this work, we present a step-by-step workflow for the fabrication of 2D hexagonal boron nitride (h-BN) nanopores which are then used to sense holo-human serum transferrin (hSTf) protein at pH ∼8 under applied voltages ranging from +100 mV to +800 mV....
Gad D Vatine et al.
Cell stem cell, 24(6), 995-1005 (2019-06-08)
The blood-brain barrier (BBB) tightly regulates the entry of solutes from blood into the brain and is disrupted in several neurological diseases. Using Organ-Chip technology, we created an entirely human BBB-Chip with induced pluripotent stem cell (iPSC)-derived brain microvascular endothelial-like cells...
Jugal Saharia et al.
ACS nano, 13(4), 4246-4254 (2019-03-08)
In this study, we investigated the voltage and pH responsiveness of human serum transferrin (hSTf) protein using silicon nitride (Si xN y) nanopores. The Fe(III)-rich holo form of hSTf was dominant when pH > pI, while the Fe(III)-free apo form...
Marcella Brescia et al.
Cells, 9(4) (2020-04-08)
Duchenne muscular dystrophy (DMD) is a fatal X-linked muscle wasting disorder arising from mutations in the ~2.4 Mb dystrophin-encoding DMD gene. RNA-guided CRISPR-Cas9 nucleases (RGNs) are opening new DMD therapeutic routes whose bottlenecks include delivering sizable RGN complexes for assessing...
Engineered mitochondrial ferritin as a magnetic resonance imaging reporter in mouse olfactory epithelium
Iordanova B, et al.
Testing, 8(8), e72720-e72720 (2013)
Yunash Maharjan et al.
EMBO reports, 21(5), e48901-e48901 (2020-03-12)
Recent evidence has linked the lysosomal cholesterol accumulation in Niemann-Pick type C1 with anomalies associated with primary ciliogenesis. Here, we report that perturbed intracellular cholesterol distribution imposed by lysosomal cholesterol accumulation during TMEM135 depletion is closely associated with impaired ciliogenesis....
Miriam Sanz-Rodriguez et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 38(22), 5096-5110 (2018-05-04)
Rapid and effective neural transmission of information requires correct axonal myelination. Modifications in myelination alter axonal capacity to transmit electric impulses and enable pathological conditions. In the CNS, oligodendrocytes (OLs) myelinate axons, a complex process involving various cellular interactions. However...
Co-operative signalling mechanisms required for erythroid precursor expansion in response to erythropoietin and stem cell factor
Arcasoy O and Jiang X
British Journal of Haematology, 130(1), 121-129 (2005)
Pamela Sarkar et al.
Cytotherapy, 20(1), 21-28 (2017-09-18)
Clinical trials using ex vivo expansion of autologous mesenchymal stromal cells (MSCs) are in progress for several neurological diseases including multiple sclerosis (MS). Given that environment alters MSC function, we examined whether in vitro expansion, increasing donor age and progressive...
Valentina Benedetti et al.
EBioMedicine, 33, 253-268 (2018-07-28)
The lack of engineering systems able to faithfully reproduce complex kidney structures in vitro has made it difficult to efficiently model kidney diseases and development. Using polydimethylsiloxane (PDMS) scaffolds and a kidney-derived cell line we developed a system to rapidly...
Özge Canli et al.
Blood, 127(1), 139-148 (2015-10-16)
Maintaining cellular redox balance is vital for cell survival and tissue homoeostasis because imbalanced production of reactive oxygen species (ROS) may lead to oxidative stress and cell death. The antioxidant enzyme glutathione peroxidase 4 (Gpx4) is a key regulator of...
Shaojun Liang et al.
Ecotoxicology and environmental safety, 183, 109498-109498 (2019-08-05)
2',2',4,4'-tetrabromo diphenyl ether (BDE-47), one of the most abundant congeners of commercial pentaBDE utilized as flame retardants, has been phased out of production due to its potential neural toxicity and endocrine disrupting activities, and yet still present in the environment....
Muralidharan Mani et al.
Molecular biology of the cell, 27(2), 334-348 (2015-11-20)
The small GTPase Rab5 regulates the early endocytic pathway of transferrin (Tfn), and Rab5 deactivation is required for Tfn recycling. Rab5 deactivation is achieved by RabGAP5, a GTPase-activating protein, on the endosomes. Here we report that recruitment of RabGAP5 is...
Jung-Feng Lin et al.
International journal of molecular sciences, 20(21) (2019-10-28)
Chronic renal failure involving hemodialysis results in blood loss during filtration. Iron deficiency and iron deficiency anemia can result. A compensatory increase in iron dosage has many side effects including discomfort. Elemental iron is a highly-pure iron source, which reduces...
Tomohiro Makino et al.
Biochemical pharmacology, 171, 113717-113717 (2019-11-22)
Heritable disorders associated with hemoglobin production are the most common monogenic disorders. These are mainly represented by disorders such as β-thalassemia and sickle cell disease. Induction of fetal hemoglobin (HbF) has been known to ameliorate the clinical severity of these...
Vijaykumar Chennupati et al.
The Journal of clinical investigation, 128(4), 1597-1614 (2018-02-07)
Ribosomal proteins (RP) regulate specific gene expression by selectively translating subsets of mRNAs. Indeed, in Diamond-Blackfan anemia and 5q- syndrome, mutations in RP genes lead to a specific defect in erythroid gene translation and cause anemia. Little is known about...
Jill M Brown et al.
Nature communications, 9(1), 3849-3849 (2018-09-23)
Self-interacting chromatin domains encompass genes and their cis-regulatory elements; however, the three-dimensional form a domain takes, whether this relies on enhancer-promoter interactions, and the processes necessary to mediate the formation and maintenance of such domains, remain unclear. To examine these...