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T36609

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C J Krieger et al.
Journal of bacteriology, 181(20), 6403-6410 (1999-10-09)
The initial enzymatic steps in anaerobic m-xylene oxidation were studied in Azoarcus sp. strain T, a denitrifying bacterium capable of mineralizing m-xylene via 3-methylbenzoate. Permeabilized cells of m-xylene-grown Azoarcus sp. strain T catalyzed the addition of m-xylene to fumarate to...
K Miura et al.
Biochemistry and molecular biology international, 46(5), 933-941 (1998-12-23)
The transcription of OP2 encoding enzymes for m-toluate catabolism on the Pseudomonas putida TOL plasmid is activated by basal-level XylS protein in the presence of m-toluate or by overproduced XylS protein in the absence of m-toluate. In this study, in...
A Cebolla et al.
Applied and environmental microbiology, 62(1), 214-220 (1996-01-01)
A simple procedure to detect the switching on and off of catabolic promoters of Pseudomonas putida, at the level of single cells based on the immunodetection of a reporter epitope expressed on the surface of bacterial cells, has been developed....
Nina Bjørk Arnfinnsdottir et al.
PloS one, 10(6), e0128162-e0128162 (2015-06-04)
In this paper we demonstrate a procedure for preparing bacterial arrays that is fast, easy, and applicable in a standard molecular biology laboratory. Microcontact printing is used to deposit chemicals promoting bacterial adherence in predefined positions on glass surfaces coated...
M A Kivisaar et al.
Journal of bacteriology, 171(9), 5111-5116 (1989-09-01)
The utilization of phenol, m-toluate, and salicylate (Phe+, mTol+, and Sal+ characters, respectively) in Pseudomonas sp. strain EST1001 is determined by the coordinated expression of genes placed in different plasmids, i.e., by a multiplasmid system. The natural multiplasmid strain EST1001...
Shiu-Mei Liu et al.
Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering, 38(6), 1099-1113 (2003-05-31)
Composition of the headspace gas affected the biotransformation pattern of toluic acid isomers in anoxic sediment slurries. Under an N2 atmosphere, o- and m-, and p-toluic acid (20-25 mg L(-1)) were biotransformed in 100 days, 77 days, and 148 days...
D Durkin et al.
Electrophoresis, 21(10), 1997-2009 (2000-07-06)
The concept of dual opposite injection in capillary electrophoresis (DOI-CE) for the simultaneous separation, under conditions of suppressed electroosmotic flow, of anionic and cationic compounds with no bias in resolution and analysis time, is extended to a higher pH range...
S Inouye et al.
Journal of bacteriology, 169(8), 3587-3592 (1987-08-01)
The effect of high-level expression of the regulatory gene xylS of the Pseudomonas putida TOL plasmid on the activation of the xylDLEGF operon was investigated in Escherichia coli. The xylS gene was placed downstream from the tac promoter, and the...
K Watanabe et al.
Drug metabolism and disposition: the biological fate of chemicals, 23(2), 261-265 (1995-02-01)
Mouse hepatic microsomal enzymes catalyzed the oxidation of o-, m-, and p-tolualdehydes, intermediate metabolites of xylene, to the corresponding toluic acids. Cofactor requirement for the catalytic activity indicates that the microsomes contain NAD- and NADPH-dependent enzymes for this reaction. GC/MS...
Jun Yao et al.
Applied and environmental microbiology, 73(8), 2735-2743 (2007-02-27)
Mobile group II introns ("targetrons") can be programmed for insertion into virtually any desired DNA target with high frequency and specificity. Here, we show that targetrons expressed via an m-toluic acid-inducible promoter from a broad-host-range vector containing an RK2 minireplicon...
C P Saint et al.
Journal of general microbiology, 136(4), 615-625 (1990-04-01)
A restriction endonuclease map was derived for the aromatic amine and m-toluate catabolic plasmid pTDN1 present in Pseudomonas putida UCC22, a derivative of P. putida mt-2. The plasmid is 79 +/- 1 kbp in size and can be divided into...
Rafael Silva-Rocha et al.
Molecular bioSystems, 8(7), 1950-1957 (2012-05-17)
In this report, we experimentally demonstrate that improving the cis-regulatory region of a target promoter can significantly enhance the response to an otherwise poor inducer. The transcriptional factor (TF) BenR of Pseudomonas putida KT2440 is a member of the AraC/XylS...
V Balachandran et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 92, 137-147 (2012-03-27)
The potential energy surface scan for the selected dihedral angle of 6-nitro-m-toluic acid (NTA) has been performed to identify stable conformer. The optimized structure parameters and vibrational wavenumbers of stable conformer have been predicted by density functional B3LYP method with...
Mihyun Bae et al.
Antonie van Leeuwenhoek, 84(1), 25-30 (2003-08-09)
A putative glutathione S-transferase (GST) gene (bphK) was identified in the meta-cleavage operon for the degradation of m-toluate by Sphingomonas yanoikuyae B1. Disruption of bphK resulted in the loss of GST activity against 1-chloro-2,4-dinitrobenzene and a much increased lag time...
A Marzo et al.
Journal of chromatography, 459, 325-329 (1988-12-28)
An high-performance liquid chromatographic analytical method, which allows quantitative evaluation of both salicyloyl pyridixol and its metabolite salicylic acid, is reported. This method has demonstrated to possess the required specifications in terms of linearity, sensitivity, extraction recovery, reproducibility and specificity...
M T Gallegos et al.
Journal of bacteriology, 178(8), 2356-2361 (1996-04-01)
Growth of Pseudomonas putida (pWWO) on alkylbenzoates requires the expression of the meta pathway operon, which is mediated by the XylS protein after binding of a benzoate effector. Alternatively, in cells growing on toluene or its aromatic alcohols, overexpression of...
G M Stephens et al.
Journal of general microbiology, 133(7), 1891-1899 (1987-07-01)
The effect of various lipophilic weak acids on the stability of certain TOL plasmids was investigated. Benzoate induced deletion of TOL plasmid DNA in Pseudomonas putida MT15, followed by loss of the plasmid; this effect was pH- and concentration-dependent, suggesting...
I Sarand et al.
Journal of applied microbiology, 86(5), 817-826 (1999-05-29)
The tolerance to, and degradation of m-toluate by Scots pine (Pinus sylvestris), a symbiotic mycorrhizal fungus (Suillus bovinus) and Pseudomonas fluorescens strains, with or without m-toluate-degrading capacity, was determined individually and in all symbiotic/associative plant-microbe combinations. Fungal survival on medium...
Yong Ge et al.
Journal of bacteriology, 184(15), 4096-4103 (2002-07-11)
Toluate dioxygenase (TADO) of Pseudomonas putida mt-2 catalyzes the dihydroxylation of a broad range of substituted benzoates. The two components of this enzyme were hyperexpressed and anaerobically purified. Reconstituted TADO had a specific activity of 3.8 U/mg with m-toluate, and...
Minna M Jussila et al.
Environmental pollution (Barking, Essex : 1987), 139(2), 244-257 (2005-08-02)
A collection of 50 indigenous meta-toluate tolerating bacteria isolated from oil-contaminated rhizosphere of Galega orientalis on selective medium was characterized and identified by classical and molecular methods. 16S rDNA partial sequencing showed the presence of five major lineages of the...
L B Jensen et al.
Applied and environmental microbiology, 59(11), 3713-3717 (1993-11-01)
A model substrate-dependent suicide system to biologically contain Pseudomonas putida KT2440 is reported. The system consists of two elements. One element carries a fusion between a synthetic lac promoter (PA1-04/03) and the gef gene, which encodes a killing function. This...
C P Saint et al.
Journal of general microbiology, 136(4), 627-636 (1990-04-01)
The ability to degrade aromatic amines and m-toluate (Tdn+ phenotype), encoded by plasmid pTDN1, was lost from Pseudomonas putida hosts after subculture in benzoate, succinate, acetate and glucose minimal medium, the fastest rate of loss occurring where benzoate was the...
R J Kolenc et al.
Applied and environmental microbiology, 54(3), 638-641 (1988-03-01)
A psychrotrophic bacterium, originally isolated from a natural aquatic environment, was characterized and identified as Pseudomonas putida Q5 for use as a representative recipient for biodegradative genes from a mesophilic microorganism. The TOL plasmid pWWO of the mesophile P. putida...
J G Bisaillon et al.
Canadian journal of microbiology, 39(7), 642-648 (1993-07-01)
An anaerobic consortium that carboxylated and dehydroxylated phenol to benzoate, and 2-cresol to 3-methylbenzoic acid, under methanogenic conditions was studied. Phenol induced this transformation activity. Addition of 4-hydroxypyridine or an increase in the concentration of proteose peptone to 0.5% (w/v)...
M Jakubowski et al.
Polish journal of occupational medicine, 2(3), 238-247 (1989-01-01)
Influence of combined exposure to solvent vapours on the metabolism of m-xylene and the kinetics of excretion of methylbenzoic acid (MBA) in urine was investigated. The volunteers were exposed in groups of four or five. Each experiment consisted of two...
S A Selifonov et al.
Biokhimiia (Moscow, Russia), 55(12), 2171-2181 (1990-12-01)
It was shown that two different enzymes of aromatic ring oxidative meta-cleavage (2,3-dihydroxybiphenyl-1,2-dioxygenase), DBO and catechol-2,3-dioxygenase, C230) function in Pseudomonas strains with a plasmid and chromosomal genetic control of biphenyl and toluate catabolism. A comparative analysis of DBO's and C230's...