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Joanna Depciuch et al.
International journal of molecular sciences, 21(23) (2020-12-03)
Herein, we propose newly designed and synthesized gold nanopeanuts (Au NPes) as supports for cisplatin (cPt) immobilization, dedicated to combined glioblastoma nano-chemo-radiotherapy. Au NPes offer a large active surface, which can be used for drugs immobilization. Transmission electron microscopy (TEM)...
József Bocsi et al.
Cytometry. Part A : the journal of the International Society for Analytical Cytology, 61(1), 1-8 (2004-09-08)
Flow cytometry (FCM) and laser scanning cytometry (LSC) are the routine techniques for fluorescent cell analysis. Recently, we developed a scanning fluorescent microscopy (SFM) technique. This study compares SFM to LSC (two slide-based cytometry, SBC, techniques) and FCM, in experimental...
Erich H Schneider et al.
Naunyn-Schmiedeberg's archives of pharmacology, 393(7), 1251-1267 (2020-04-22)
The effects of 100 μM of 3',5'-cGMP, cAMP, cCMP, and cUMP as well as of the corresponding membrane-permeant acetoxymethyl esters on anti-CD3-antibody (OKT3)-induced IL-2 production of HuT-78 cutaneous T cell lymphoma (Sézary lymphoma) cells were analyzed. Only 3',5'-cGMP significantly reduced IL-2...
Adrian Fischer et al.
Nature communications, 11(1), 3068-3068 (2020-06-20)
Surgical adhesions are bands of scar tissues that abnormally conjoin organ surfaces. Adhesions are a major cause of post-operative and dialysis-related complications, yet their patho-mechanism remains elusive, and prevention agents in clinical trials have thus far failed to achieve efficacy....
Krzysztof Kolmus et al.
Journal of cell science, 134(1) (2021-01-10)
Molecular details of how endocytosis contributes to oncogenesis remain elusive. Our in silico analysis of colorectal cancer (CRC) patients revealed stage-dependent alterations in the expression of 112 endocytosis-related genes. Among them, transcription of the endosomal sorting complex required for transport...
L Gabriel Sanchez-Partida et al.
PloS one, 6(6), e21597-e21597 (2011-07-09)
Oocyte cryopreservation is extremely beneficial for assisted reproductive technologies, the treatment of infertility and biotechnology and offers a viable alternative to embryo freezing and ovarian grafting approaches for the generation of embryonic stem cells and live offspring. It also offers...
Hiroki Kannen et al.
International journal of molecular sciences, 15(7), 11234-11244 (2014-06-27)
Recently, combined therapy using chemotherapy and photodynamic therapy (PDT) has been proposed as a means of improving treatment outcomes. In order to evaluate the efficacy of combined therapy, it is necessary to determine the distribution of the anticancer drug and...
Liqin Cao et al.
PLoS genetics, 5(12), e1000756-e1000756 (2009-12-10)
In mammals, observations of rapid shifts in mitochondrial DNA (mtDNA) variants between generations have led to the creation of the bottleneck theory for the transmission of mtDNA. The bottleneck could be attributed to a marked decline of mtDNA content in...
Yusuke Higashi et al.
Cellular signalling, 57, 10-20 (2019-02-05)
Minocycline, a tetracycline antibiotic, is known to exert vasculoprotective effects independent of its anti-bacterial properties; however the underlying molecular mechanisms are not completely understood. Reversion Inducing Cysteine Rich Protein with Kazal Motifs (RECK) is a cell surface expressed, membrane anchored...
Jovana Bisevac et al.
Scientific reports, 10(1), 20532-20532 (2020-11-27)
Late spontaneous in-the-bag intraocular lens (IOL) dislocation is a complication presenting 6 months or later after cataract surgery. We aimed to characterize the cells in the lens capsules (LCs) of 18 patients with spontaneous late in-the-bag IOL dislocation. Patients' average age...
Gürhan Özkayar et al.
Micromachines, 11(11) (2020-11-05)
Being one of the major pillars of liquid biopsy, isolation and characterization of circulating tumor cells (CTCs) during cancer management provides critical information on the evolution of cancer and has great potential to increase the success of therapies. In this...
Pawel Mroz et al.
Cancer letters, 282(1), 63-76 (2009-04-07)
The in vitro photodynamic therapy activity of four imidazole-substituted metalloporphyrins has been studied using human (HeLa) and mouse (CT26) cancer cell lines: an anionic Zn porphyrin and a homologous series of three cationic Zn, Pd or InCl porphyrins. A dramatic...
Kenta Shinha et al.
Micromachines, 11(7) (2020-07-28)
Numerous in vitro studies have been conducted in conventional static cell culture systems. However, most of the results represent an average response from a population of cells regardless of their local microenvironment. A microfluidic probe is a non-contact technology that...
Wishrawana S Ratnayake et al.
International journal of oncology, 51(5), 1370-1382 (2017-10-20)
Atypical protein kinase Cs (aPKC) are involved in cell cycle progression, tumorigenesis, cell survival and migration in many cancers. We believe that aPKCs play an important role in cell motility of melanoma by regulating cell signaling pathways and inducing epithelial...
Yan Liu et al.
Nature communications, 8(1), 643-643 (2017-09-25)
Studies of heterochronic parabiosis demonstrated that with age, the composition of the circulatory milieu changes in ways that broadly inhibit tissue regenerative capacity. In addition, local tissue niches have age-specific influences on their resident stem cells. Here we use bio-orthogonal...
Gamal M K Mehaisen et al.
PloS one, 12(2), e0172684-e0172684 (2017-02-25)
The massive meat production of broiler chickens make them continuously exposed to potential stressors that stimulate releasing of stress-related hormones like corticosterone (CORT) which is responsible for specific pathways in biological mechanisms and physiological activities. Therefore, this research was conducted...
Ivan Crespo-Enriquez et al.
Development (Cambridge, England), 146(14) (2019-07-31)
In human, mutations of the protocadherins FAT4 and DCHS1 result in Van Maldergem syndrome, which is characterised, in part, by craniofacial abnormalities. Here, we analyse the role of Dchs1-Fat4 signalling during osteoblast differentiation in mouse. We show that Fat4 and...
Alba Timón-Gómez et al.
STAR protocols, 1(2) (2020-10-01)
By using negatively charged Coomassie brilliant blue G-250 dye to induce a charge shift on proteins, blue native polyacrylamide gel electrophoresis (BN-PAGE) allows resolution of enzymatically active multiprotein complexes extracted from cellular or subcellular lysates while retaining their native conformation....
Hiroshi Sunami et al.
Advanced biosystems, 4(10), e2000113-e2000113 (2020-09-15)
The present study demonstrates unidirectional cell migration using a novel 3D microfabricated scaffold, as revealed by the uneven sorting of cells into an area of 1 mm × 1 mm. To induce unidirectional cell migration, it is important to determine the optimal...
Ling Xue et al.
The Journal of nutritional biochemistry, 19(5), 336-344 (2007-08-21)
3,3'-Diindolylmethane (DIM), a major condensation product of indole-3-carbinol, exhibits chemopreventive properties in animal models of cancer. Recent studies have shown that DIM stimulates interferon-gamma (IFN-gamma) production and potentiates the IFN-gamma signaling pathway in human breast cancer cells via a mechanism...
Mark E Corkins et al.
Genes, 9(4) (2018-04-13)
Xenopus laevis embryos are an established model for studying kidney development. The nephron structure and genetic pathways that regulate nephrogenesis are conserved between Xenopus and humans, allowing for the study of human disease-causing genes. Xenopus embryos are also amenable to...
Ayesha Kashmala Ghauri et al.
Pakistan journal of medical sciences, 36(5), 946-951 (2020-07-25)
The purpose of our study was isolation of umbilical cord blood derived mesenchymal stem (UCB-MSCs), their direct differentiation towards keratinocytes without using feeder layers, cAMP inducers and hormones known for morphological maintenance and proliferation of keratinocytes and characterization of UCB-MSCs...
Fatemeh Masjedi et al.
The Journal of steroid biochemistry and molecular biology, 197, 105521-105521 (2019-11-11)
Polycystic ovary syndrome (PCOS) is accompanied with many disturbances in hormone synthesis and antioxidant defense. Previous reports have indicated that Vitamin D (vit.D) affects gene expression and have roles in normal follicular development. Therefore, we investigated the effects of vit.D...
Timothy S Cliff et al.
Cell stem cell, 21(4), 502-516 (2017-10-03)
As human pluripotent stem cells (hPSCs) exit pluripotency, they are thought to switch from a glycolytic mode of energy generation to one more dependent on oxidative phosphorylation. Here we show that, although metabolic switching occurs during early mesoderm and endoderm...
Doris M Campos et al.
Biomatter, 3(2) (2013-05-31)
We have recently developed new 3D hydroxyapatite/collagen (50/50 wt%) scaffolds using a biomimetic synthesis approach. The first in vitro tests performed in static culture showed a limited cell colonization and survival inside the scaffolds. The current study evaluated in dynamic...
Ann Kristin Hansen et al.
BMC musculoskeletal disorders, 18(1), 432-432 (2017-11-08)
The aim was to investigate whether resident chondrocytes in human articular cartilage and in subculture express vitamin D receptor (VDR) and the enzyme that hydroxylates the prohormone 25(OH)D Cartilage from human osteoarthritic knee joints, cultured chondrocytes and cells grown in...
Rafal P Piprek et al.
The International journal of developmental biology, 63(11-12), 615-621 (2019-01-01)
Mechanisms governing differentiation of the bipotential gonad into the testes or ovaries are complex and still vague. The primary cilium is an organelle involved in cell signaling, which controls the development of many organs, but the role of primary cilium...
Dong Li et al.
Cell cycle (Georgetown, Tex.), 16(11), 1070-1084 (2017-04-21)
Previous research has shown that a subpopulation of cells within cultured human dermal fibroblasts, termed multilineage-differentiating stress enduring (Muse) cells, are preferentially reprogrammed into induced pluripotent stem cells. However, controversy exists over whether these cells are the only cells capable...
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