Showing 1-30 of 794 results for "

T8787

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Jeong Hwan Lee et al.
Plant methods, 13, 42-42 (2017-05-26)
Binding of transcription factors to their target sequences is a primary step in the regulation of gene expression and largely determines gene regulatory networks. Chromatin immunoprecipitation (ChIP) is an indispensable tool used to investigate the binding of DNA-binding proteins (e.g....
Ki-Bum Kim et al.
Methods in molecular biology (Clifton, N.J.), 424, 413-422 (2008-03-29)
Because lipid rafts are plasma membrane platforms mediating various cellular events such as in signal transduction, immunological response, pathogen invasion, and neurodegenerative diseases, protein identification in the rafts could provide important information to study their function. Here, we present an...
Winco Wh Wu et al.
Virology journal, 6, 68-68 (2009-06-06)
Early in infection, the genome of the influenza A virus, consisting of eight complexes of RNA and proteins (termed viral ribonucleoproteins; vRNPs), enters the nucleus of infected cells for replication. Incoming vRNPs are imported into the nucleus of infected cells...
Jianmin Zhao et al.
Stem cells and development, 26(7), 528-540 (2016-12-09)
The epicardium promotes neovascularization and cardiomyocyte regeneration by generating vascular smooth muscle cells (SMCs) and producing regenerative factors after adult heart infarction. It is therefore a potential cell resource for repair of the injured heart. However, the epicardium also participates...
Angélique Jimenez et al.
Methods (San Diego, Calif.), 174, 100-114 (2019-05-13)
Super-resolution microscopy has profoundly transformed how we study the architecture of cells, revealing unknown structures and refining our view of cellular assemblies. Among the various techniques, the resolution of Single Molecule Localization Microscopy (SMLM) can reach the size of macromolecular...
Wan Zhu et al.
Autophagy, 10(9), 1622-1636 (2014-07-22)
Macroautophagy is a vesicular catabolic trafficking pathway that is thought to protect cells from diverse stressors and to promote longevity. Recent studies have revealed that transcription factors play important roles in the regulation of autophagy. In this study, we have...
Justin A MacDonald et al.
Archives of biochemistry and biophysics, 535(1), 84-90 (2012-12-12)
The protein prostate-apoptosis response (Par)-4 has been implicated in the regulation of smooth muscle contraction, based largely on studies with the A7r5 cell line. A mechanism has been proposed whereby Par-4 binding to MYPT1 (the myosin-targeting subunit of myosin light...
Jean-Sébastien Joyal et al.
Blood, 117(22), 6024-6035 (2011-03-01)
The failure of blood vessels to revascularize ischemic neural tissue represents a significant challenge for vascular biology. Examples include proliferative retinopathies (PRs) such as retinopathy of prematurity and proliferative diabetic retinopathy, which are the leading causes of blindness in children...
S Tsutsumi et al.
Oncogene, 27(17), 2478-2487 (2007-10-31)
Heat shock protein 90 (Hsp90) is a molecular chaperone that maintains function of numerous intracellular signaling nodes utilized by cancer cells for proliferation and survival. Hsp90 is also detected on the plasma membrane of tumor cells and its expression has...
Clara Morral et al.
STAR protocols, 1(2), 100103-100103 (2020-10-02)
Here, we describe a protocol to detect and visualize protein synthesis by click-chemistry-based immunofluorescence in patient-derived organoids (PDOs) in vitro. The protocol uses O-propargyl puromycin (OPP), an analog of puromycin that enters the acceptor site of ribosomes and is incorporated...
Vincent Truong et al.
SLAS technology, 26(3), 287-299 (2020-12-10)
Derivation and differentiation of human induced pluripotent stem cells (hiPSCs) provide the opportunity to generate medically important cell types from individual patients and patient populations for research and the development of potential cell therapies. This technology allows disease modeling and...
Jae-Heon Kim et al.
Dental materials : official publication of the Academy of Dental Materials, 36(7), 945-958 (2020-06-02)
Titanium and its alloys are widely used for dental and medical biomaterials due to their excellent mechanical and biological advantages. After the introduction of direct laser metal sintering (DLMS) 3D printing technology and its use over conventional machine-cut processes, questions...
Mads Gabrielsen et al.
Molecular cell, 68(2), 456-470 (2017-10-21)
RING and U-box E3 ubiquitin ligases regulate diverse eukaryotic processes and have been implicated in numerous diseases, but targeting these enzymes remains a major challenge. We report the development of three ubiquitin variants (UbVs), each binding selectively to the RING...
Davide Visigalli et al.
Frontiers in neurology, 11, 903-903 (2020-09-29)
In Charcot-Marie-Tooth type 1A (CMT1A), Schwann cells exhibit a preponderant transcriptional deficiency of genes involved in lipid biosynthesis. This perturbed lipid metabolism affects the peripheral nerve physiology and the structure of peripheral myelin. Nevertheless, the identification and functional characterization of...
Marie Billaud et al.
PloS one, 4(7), e6432-e6432 (2009-08-04)
Endothelial control of vascular smooth muscle plays a major role in the resulting vasoreactivity implicated in physiological or pathological circulatory processes. However, a comprehensive understanding of endothelial (EC)/smooth muscle cells (SMC) crosstalk is far from complete. Here, we have examined...
Jing Cui et al.
Experimental eye research, 88(3), 438-444 (2008-11-27)
Previous investigators reported that epiretinal membranes isolated from patients with proliferative vitreoretinopathy (PVR) express various platelet-derived growth factor (PDGF) family members and PDGF receptors (PDGFRs) (Cui, J.Z., Chiu, A., Maberley, D., Ma, P., Samad, A., Matsubara, J.A., 2007. Stage specificity...
Mehrdad Alirezaei et al.
Autophagy, 11(8), 1389-1407 (2015-06-20)
RNA viruses modify intracellular membranes to produce replication scaffolds. In pancreatic cells, coxsackievirus B3 (CVB3) hijacks membranes from the autophagy pathway, and in vivo disruption of acinar cell autophagy dramatically delays CVB3 replication. This is reversed by expression of GFP-LC3...
Changhan Ouyang et al.
Autophagy, 16(10), 1753-1770 (2019-12-28)
Macroautophagy/autophagy, a fundamental process for degradation of macromolecules and organelles, occurs constitutively at a basal level and is upregulated in response to stress. Whether autophagy regulates protein acetylation and microtubule stability in vascular smooth muscle cells (VSMCs) migration, however, remains...
Steve M Nelson et al.
Developmental biology, 328(1), 24-39 (2009-02-13)
Retinal homeobox (Rx/Rax) genes are essential for the organogenesis of the vertebrate eye. These genes are dynamically expressed in a tissue-specific manner during eye development, suggesting pleiotropic roles. We use a temporally-selective gene knockdown approach to identify endogenous functions for...
Denise A B Smith et al.
Ultrasound in medicine & biology, 36(1), 145-157 (2009-11-11)
Echogenic liposomes (ELIP) were developed as ultrasound-triggered targeted drug or gene delivery vehicles (Lanza et al. 1997; Huang et al. 2001). Recombinant tissue-type plasminogen activator (rt-PA), a thrombolytic, has been loaded into ELIP (Tiukinhoy-Laing et al. 2007). These vesicles have...
Aurélie Morin et al.
Cell reports, 30(13), 4551-4566 (2020-04-03)
Loss-of-function mutations in the SDHB subunit of succinate dehydrogenase predispose patients to aggressive tumors characterized by pseudohypoxic and hypermethylator phenotypes. The mechanisms leading to DNA hypermethylation and its contribution to SDH-deficient cancers remain undemonstrated. We examine the genome-wide distribution of 5-methylcytosine and...
Pawel Leznicki et al.
The Biochemical journal, 436(3), 719-727 (2011-04-07)
TA (tail-anchored) proteins utilize distinct biosynthetic pathways, including TRC40 (transmembrane domain recognition complex of 40 kDa)-mediated, chaperone-dependent and/or unassisted routes to the ER (endoplasmic reticulum) membrane. We have addressed the flexibility of cytosolic components participating in these pathways, and explored...
Carla Maria Figueiredo de Carvalho Miranda et al.
Journal of equine veterinary science, 90, 102962-102962 (2020-06-15)
Equine represents an attractive animal model for musculoskeletal tissue diseases, exhibiting much similarity to the injuries that occur in humans. Cell therapy and tissue bioengineering have been widely used as a therapeutic alternative by regenerative medicine in musculoskeletal diseases. Thus...
Rajeev Gupta et al.
Cell stem cell, 26(4), 527-541 (2020-03-21)
Umbilical cord blood (UCB) has had considerable impact in pediatric stem cell transplantation, but its wider use is limited in part by unit size. Long-term ex vivo culture offers one approach to increase engraftment capacity by seeking to expand stem and...
Brian C Syverud et al.
Tissue engineering. Part A, 22(5-6), 480-489 (2016-01-23)
Tissue engineered skeletal muscle has potential for application as a graft source for repairing soft tissue injuries, a model for testing pharmaceuticals, and a biomechanical actuator system for soft robots. However, engineered muscle to date has not produced forces comparable...
Yu Shen et al.
Journal of neurochemistry, 152(1), 72-91 (2019-09-29)
Peripheral nerve injury elicits spinal microgliosis, contributing to neuropathic pain. The aurora kinases A (AURKA), B (AURKB), and C (AURKC) are potential therapeutic targets in proliferating cells. However, their role has not been clarified in microglia. The aim of this...
Michael J Bertoldo et al.
Cell reports, 30(6), 1670-1681 (2020-02-13)
Reproductive aging in female mammals is an irreversible process associated with declining oocyte quality, which is the rate-limiting factor to fertility. Here, we show that this loss of oocyte quality with age accompanies declining levels of the prominent metabolic cofactor...
Ugo Dionne et al.
Molecular cell, 70(6), 995-1007 (2018-06-19)
Phosphotyrosine (pTyr) signaling has evolved into a key cell-to-cell communication system. Activated receptor tyrosine kinases (RTKs) initiate several pTyr-dependent signaling networks by creating the docking sites required for the assembly of protein complexes. However, the mechanisms leading to network disassembly...
Hyun-Jong Cho et al.
Small (Weinheim an der Bergstrasse, Germany), 17(14), e2007425-e2007425 (2021-03-11)
Despite considerable efforts in modeling liver disease in vitro, it remains difficult to recapitulate the pathogenesis of the advanced phases of non-alcoholic fatty liver disease (NAFLD) with inflammation and fibrosis. Here, a liver-on-a-chip platform with bioengineered multicellular liver microtissues is...
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