Showing 1-30 of 58 results for "W4502"
Chromatin Isolation by RNA Purification (ChIRP) Protocol
Protocol for Chromatin Isolation by RNA Purification (ChIRP) Protocol
Standard Reverse Transcription Protocol (Two-step)
reverse transcription is the analysis of gene expression to measure concentration mrna a gene. there are several challenges such analyses, as differences in halflife between different transcripts, temporal patterns and lack correlation protein.
SeqPlex Enhanced DNA Amplification Kit (SEQXE) Protocol
The SeqPlex DNA Amplification Kit for whole genome amplification (WGA) is designed to facilitate next-generation sequencing (NGS) from extremely small quantities or from degraded/highly fragmented DNA
Qualitative multiplex PCR assay for assessing DNA quality from FFPE tissues and other sources of damaged DNA
The assessment of DNA quality is a crucial first step in acquiring meaningful data from formalin-fixed paraffin-embedded (FFPE) tissues, and other sources of damaged DNA. Using intact genomic DNA is key for successful analysis of chromosomal aberrations (e.g. SNP analysis...
Reverse Transcription Protocol (One-step Probe Detection)
Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.
Cloning Genes-of-Interest into a Plasmid Vector
This cloning protocol includes selecting the cloning system and plasmid vector, plasmid restriction digestion, fragment restriction digestion, gel excision, dephosphorylating DNA and more.
Integration of Sigma® TransPlex® WTA and the Complete WTA2 Kits with the NimbleGen™ Expression Microarray Workflow
Amplification products generated by the TransPlex® WTA and Complete WTA2 kits are suitable for microarray target for expression analyses, and can be readily integrated into existing NimbleGen workflows.
CRISPR/CAS9 Gene Editing Protocol for Human Induced Pluripotent Stem Cells (iPSCs)
This article provides an extensive step-wise overview of CRISPR Cas9 protocol that can be used to perform gene editing in human induced pluripotent stem cells (iPSCs).
Restriction Enzyme Cloning Manual Buffer Recipes
TE Buffer; Elution Buffer; 10x Ligation Buffer; 0.5 M PIPES Buffer; Inoue Transformation Buffer
Plant DNA Extraction & WGA Amplification Protocol
GenomePlex® Whole Genome Amplification has been used to amplify genomic DNA from soybean, corn, tomato, purple coneflower, and ginseng.
SEQR - SeqPlex RNA Amplification Kit Protocol
The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.
Imprint® Methylated DNA Quantification Kit (MDQ1) Protocol
Imprint <sup>®</sup> Methylated DNA Quantification Kit (<a href="#">MDQ1</a>) Protocol
Whole Genome Amplification: Extraction Protocol - Blood Card
Whole Genome Amplification: Extraction Protocol - Blood cards provide the convenience of archiving small volumes of blood. However, many times genomic DNA from these samples is limited, which may hinder the researcher’s ability to perform downstream analysis. This protocol provides...
Whole Blood
Whole blood is a common source of material used to perform genetic analysis. Many times genomic DNA isolated from whole blood samples is of low yield.
Integration of Sigma® TransPlex® WTA and Complete WTA2 Kits with the Agilent Microarray Workflow
Amplification products generated by the TransPlex® WTA and Complete WTA2 kits are suitable for microarray target for expression analyses, and can be readily integrated into existing Agilent workflows
GenomePlex Whole Genome Amplification Kit Protocol (WGA1)
GenomePlex is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative, 500-fold amplification of genomic DNA
Reverse Transcription Protocol (One-step SYBR Green I Dye Detection)
Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.
Calcium Phosphate Transfection Kit Protocol
Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.
Integration of Sigma® TransPlex® WTA and the Complete WTA2 Kits with the Affymetrix Microarray Workflow
TransPlex WTA amplification product is suitable as microarray target for expression analysis on the Affymetrix platform, and can be incorporated into existing Affymetrix workflows
qPCR Using a Single Detection Probe Protocol
A protocol that can be used as a basic template for qPCR incorporating a detection probe that is specific to a single target. In these reactions, primers and probe are included at a final concentration of 200 nM and are...
SPUD Assay for Detection of Assay Inhibitors Protocol
he SPUD assay is one option for identification of inhibitors that may be present in RNA or DNA samples. The assay is particularly useful when a large number of samples are to be analyzed or when targets are present at...
GenomePlex Whole Genome Amplification Kit Protocol (WGA1)
GenomePlex is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative, 500-fold amplification of genomic DNA
Integration of Sigma® TransPlex® WTA and the Complete WTA2 Kits with the Illumina Microarray Workflow
Amplification products generated by the TransPlex® WTA and Complete WTA2 kits are suitable for microarray target for expression analyses, and can be incorporated into existing Illumina workflows.
qPCR Gene Expression Using Probe Detection
The most common application for qPCR is the measurement of a gene transcript or copy number quantity relative to one or more reference genes using probe detection.
Multiplex qPCR Protocol
A protocol that can be used as a basic template for qPCR incorporating up to four detection probes. In these reactions, primers and probe are included at a final concentration of 200 nM and are run using LuminoCt ReadyMix.
The 3'/5' Assay for Analysis of RNA Integrity Protocol
The 3’/5’ integrity assay is a potential first step in the identification of RNA degradation. The assay is particularly useful when a large number of samples are to be analyzed or when the degradation is less than that detected by...
Complete Whole Transcriptome Amplification Kit Protocol (WTA2)
WTA2, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3-bias
FFPE Tissue
Archived Formalin-fixed, Paraffin-embedded (FFPE) tissue samples are invaluable resources for profiling gene expression and studying a variety of diseases.
qPCR Gene Expression/Copy Number Analysis Protocol Using SYBR Green I Dye Detection
Measuring a target quantity relative to one or more stable reference genes using SYBR Green I dye detection is a common application of qPCR. Below is a standard protocol that can be adapted to specific experimental needs.
Primer Optimization Protocol Using Temperature Gradient
Gradient PCR for assay optimization is to determine the optimum annealing temperature (Ta) of the primers by testing identical reactions containing a fixed primer concentration, across a range of annealing temperatures.

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