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Showing 1-30 of 34 results for "W4502SIGMA" within Site Content
Imprint® Methylated DNA Quantification Kit (MDQ1) Protocol
Imprint ® Methylated DNA Quantification Kit (MDQ1) Protocol
GenomePlex® Single Cell Whole Genome Amplification Kit (WGA4) - Protocol
GenomePlex® is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative amplification of genomic DNA
Complete Whole Transcriptome Amplification Kit Protocol (WTA2)
WTA2, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3-bias
Preparation of Antibody Sensitized Sheep Erythrocytes
Protocol for the preparation of Antibody Sensitized Sheep Erythrocytes. Includes product numbers and links.
Saliva DNA Extraction & WGA Amplification
Whole Genome Amplification products, including kits for DNA extraction, support various DNA sources.
Animal Tissue DNA-Extraction & WGA Amplification Protocol
GenomePlex® Whole Genome Amplification efficiently extracts DNA from animal samples for genomic analysis.
GenElute™ Plant Genomic DNA Miniprep Kit Protocol (G2N10, G2N70, G2N350)
This protocol describes a simple and convenient procedure to isolate pure DNA from a variety of plant species using the GenElute Plant Genomic DNA Miniprep Kit.
Whole Genome Amplification from Serum or Plasma Protocol
Whole genome amplification (WGA) of plasma and serum DNA presents a unique challenge due to the small amount of nucleic acid in such samples.
Blood Card - WGA Protocol
Protocol extracts genomic DNA from blood cards, useful for limited DNA samples in amplification.
Extraction & Amplification of Whole Blood Using WGA-Protocol
Simple DNA isolation protocol from whole blood samples for genomic analysis, followed by GenomePlex® amplification.
Reverse Transcription Protocol
Method for reverse transcription of RNA into DNA. Uses a premixed reagent that contains reverse transcriptase, dNTPs, primers, RNase inhibitor and buffer. Fast generation of cDNA.
SEQR - SeqPlex RNA Amplification Kit Protocol
The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.
Buccal DNA Extraction & WGA Amplification Protocol
This protocol provides a simple and convenient method to isolate, amplify, and purify genomic DNA from buccal swabs. Buccal swabs are a convenient method of acquiring a DNA sample.
Reverse Transcription Protocol (One-step Probe Detection)
Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.
Plant DNA Extraction & WGA Amplification Protocol
GenomePlex® Whole Genome Amplification has been used to amplify genomic DNA from soybean, corn, tomato, purple coneflower, and ginseng.
Reverse Transcription Protocol Using SYBR Green Dye Detection
Perform reverse transcription (RT) using a reverse transcriptase enzyme and dNTPs. Use total RNA or a gene-specific approach so that only the RNA of interest is converted to cDNA.
Standard Reverse Transcription Protocol
Reverse transcription analyzes mRNA gene expression, facing challenges like transcript half-life differences and temporal patterns.
Multiplex qPCR Protocol
Standard qPCR protocol with up to four detection probes at specified concentrations, simplifying reaction setup with LuminoCt ReadyMix.
Primer Concentration Optimization
Primer Concentration Optimization Protocol creates reaction matrix for testing primer concentrations against various partners.
qPCR with Single Detection Probe
qPCR protocol template with specific detection probes aids in single-target detection using LuminoCt® ReadyMix™.
GenElute™ Blood Genomic DNA Kit Protocol
The GenElute™ Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.
GenElute™ Mammalian Genomic DNA Miniprep Kit Protocol
The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.
Restriction Enzyme Cloning Manual Buffer Recipes
TE Buffer; Elution Buffer; 10x Ligation Buffer; 0.5 M PIPES Buffer; Inoue Transformation Buffer
GenElute™ Bacterial Genomic DNA Kit Protocol (NA2100, NA2110, NA2120)
GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.
The 3'/5' Assay for Analysis of RNA Integrity Protocol
The 3’/5’ integrity assay identifies RNA degradation, crucial for large sample sets or less detectable degradation.
Primer Optimization Using Temperature Gradient
Gradient PCR optimizes assay conditions by testing fixed primer concentrations across various annealing temperatures.
qPCR Efficiency Determination Protocol
Optimization of qPCR conditions is important for the development of a robust assay. The two main approaches are optimization of primer concentration and/or annealing temperatures.
qPCR Gene Expression Protocol Using SYBR Green
SYBR Green I dye in qPCR measures target quantity, adaptable to specific needs with a standard protocol.
qPCR Reference Gene Selection Protocol
Analysis of gene expression data requires a stable reference or loading control. This reference is usually one or more reference genes.
SPUD Assay for Detection of Assay Inhibitors Protocol
SPUD assay identifies inhibitors in RNA or DNA samples, useful for analyzing numerous or low-copy targets.
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