Showing 1-30 of 54 results for "WGA2"
Comparing Whole-Genome Amplification Methods and Sources for Biological Samples for Single-Nucleotide Polymorphism Genotyping
Park, J. W., et al.
Clinical Chemistry, 51(8), 1520-1523 (2004)
Trong Nguyen Duc et al.
Methods in molecular biology (Clifton, N.J.), 911, 491-505 (2012-08-14)
Chromatin immunoprecipitation (ChIP), followed by microarray hybridization (ChIP-chip) or high-throughput sequencing (ChIP-seq), is becoming a widely used powerful method for the analysis of the in vivo DNA-protein interactions at genomic scale.The success of ChIP largely depends on the quality of...
Karen Jung et al.
Breast cancer research : BCR, 16(6), 470-470 (2014-11-09)
Aberrant expression of the embryonic stem cell marker Sox2 has been reported in breast cancer (BC). We previously identified two phenotypically distinct BC cell subsets separated based on their differential response to a Sox2 transcription activity reporter, namely the reporter-unresponsive...
Kunio Kitada et al.
Genes, chromosomes & cancer, 50(4), 217-227 (2011-02-15)
Analysis of structural rearrangements at the individual chromosomal level is still technologically challenging. Here we optimized a chromosome isolation method using fluorescent marker-assisted laser-capture and laser-beam microdissection and applied it to structural analysis of two aberrant chromosomes found in a...
Susan Gribble et al.
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 12(2), 143-151 (2004-04-01)
We have used OmniPlex library technology to construct chromosome painting probes from single copies of flow sorted chromosomes. We show that this whole genome amplification technology is particularly efficient at amplifying single copies of chromosomes for the production of paints...
Nan Jiang et al.
Molecular and cellular biology, 29(13), 3556-3568 (2009-04-22)
Insulator elements play a role in gene regulation that is potentially linked to nuclear organization. Boundary element-associated factors (BEAFs) 32A and 32B associate with hundreds of sites on Drosophila polytene chromosomes. We hybridized DNA isolated by chromatin immunoprecipitation to genome...
Nadine L Vastenhouw et al.
Nature, 464(7290), 922-926 (2010-03-26)
After fertilization the embryonic genome is inactive until transcription is initiated during the maternal-zygotic transition. This transition coincides with the formation of pluripotent cells, which in mammals can be used to generate embryonic stem cells. To study the changes in...
Combinatorial interactions are required for the efficient recruitment of pho repressive complex (PhoRC) to polycomb response elements.
Kahn TG, Stenberg P, Pirrotta V, et al.
PLoS Genetics, 10(7), e1004495-e1004495 (2014)
Seth Frietze et al.
PloS one, 5(12), e15082-e15082 (2010-12-21)
Only a small percentage of human transcription factors (e.g. those associated with a specific differentiation program) are expressed in a given cell type. Thus, cell fate is mainly determined by cell type-specific silencing of transcription factors that drive different cellular...
Deletion of a conserved cis-element in the Ifng locus highlights the role of acute histone acetylation in modulating inducible gene transcription.
Balasubramani A, Winstead CJ, Turner H, et al.
PLoS Genetics, 10(1), e1003969-e1003969 (2014)
Junfang Zhang et al.
Journal of virology, 86(19), 10524-10532 (2012-07-20)
Retroviruses integrated into genomic DNA participate in long-range gene activation from as far away as several hundred kilobases. Hypotheses have been put forth to account for these phenomena, but data have not been provided to support a physical mechanism that...
Genome-wide methylation analysis of tubulocystic and papillary renal cell carcinomas.
Korabecna M, et al.
Neoplasma, 63, 402-410 (2016)
C Selmi et al.
Clinical and experimental immunology, 169(3), 253-262 (2012-08-07)
Scleroderma (SSc) is a rare connective tissue disease characterized by fibrosis, microvasculopathy and autoimmune features. The role of genetics is limited in SSc, as suggested by similar concordance rates in monozygotic and dizygotic twin pairs, while environmental factors may act...
Uwe Ueberham et al.
Neurobiology of aging, 33(12), 2827-2840 (2012-03-16)
Alzheimer's disease (AD) is a neurodegenerative disorder characterized by deregulation of neuronal cell cycle and differentiation control eventually resulting in cell death. During brain development, neuronal differentiation is regulated by Smad proteins, which are elements of the canonical transforming growth...
Tao Chen et al.
Toxicology, 292(2-3), 63-70 (2011-11-15)
Furan, a widely used industrial compound, has been found in a number of heated food items. Furan is carcinogenic to rats and mice, but the mechanism behind its carcinogenic effect is still not well understood. In this study, we tested...
M Korabecna et al.
Neoplasma, 63(3), 402-410 (2016-03-02)
Tubulocystic renal cell carcinoma (TRCC) represents a rare tumor with incidence lower than 1 % of all renal carcinomas. This study was undertaken to contribute to characterization of molecular signatures associated with TRCC and to compare them with the features of papillary...
Annalisa Vetro et al.
European journal of medical genetics, 55(3), 185-190 (2012-02-22)
Traditional approaches for the classification of Small Supernumerary Marker Chromosomes (sSMC), mostly based on FISH techniques, are time-consuming and not always sufficient to fully understand the true complexity of this class of rearrangements. We describe four supernumerary marker chromosomes that...
Victoria Louise Chapman et al.
Toxicological sciences : an official journal of the Society of Toxicology, 139(2), 350-361 (2014-03-29)
Epigenetic regulation of gene expression plays a pivotal role in the orchestration of immune responses and may determine the vigor, quality, or longevity of such responses. Chemical allergens can be divided into two categories: skin sensitizing chemicals associated with allergic...
Wing-Kit Yip et al.
PloS one, 6(8), e22888-e22888 (2011-08-11)
The biological pathways and functional properties by which misexpressed microRNAs (miRNAs) contribute to liver carcinogenesis have been intensively investigated. However, little is known about the upstream mechanisms that deregulate miRNA expressions in this process. In hepatocellular carcinoma (HCC), hepatitis B...
Lara Rajeev et al.
Genome biology, 12(10), R99-R99 (2011-10-14)
Two component regulatory systems are the primary form of signal transduction in bacteria. Although genomic binding sites have been determined for several eukaryotic and bacterial transcription factors, comprehensive identification of gene targets of two component response regulators remains challenging due...
Pål A Olsvik et al.
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 165, 17-27 (2014-06-01)
This study aimed to investigate whether dioxin (TCDD) and methylmercury (MeHg) pose a threat to offspring of fish exposed to elevated concentrations of these chemicals via epigenetic-based mechanisms. Adult female zebrafish were fed diets added either 20 μg/kg 2,3,7,8 TCDD...
Y Itzhak et al.
Molecular psychiatry, 20(2), 232-239 (2014-02-19)
The high rate of methamphetamine (METH) abuse among young adults and women of childbearing age makes it imperative to determine the long-term effects of METH exposure on the offspring. We hypothesized that parental METH exposure modulates offspring behavior by disrupting...
dREAM co-operates with insulator-binding proteins and regulates expression at divergently paired genes.
Korenjak M, Kwon E, Morris RT, et al.
Nucleic Acids Research, 42(14), 8939-8953 (2014)
Levi Yant et al.
The Plant cell, 22(7), 2156-2170 (2010-08-03)
The Arabidopsis thaliana transcription factor APETALA2 (AP2) has numerous functions, including roles in seed development, stem cell maintenance, and specification of floral organ identity. To understand the relationship between these different roles, we mapped direct targets of AP2 on a...
David L Barker et al.
Genome research, 14(5), 901-907 (2004-05-05)
Comprehensive genome scans involving many thousands of SNP assays will require significant amounts of genomic DNA from each sample. We report two successful methods for amplifying whole-genomic DNA prior to SNP analysis, multiple displacement amplification, and OmniPlex technology. We determined...
Michelle M Thiaville et al.
PloS one, 7(5), e36054-e36054 (2012-05-09)
PBX1 is a TALE homeodomain transcription factor involved in organogenesis and tumorigenesis. Although it has been shown that ovarian, breast, and melanoma cancer cells depend on PBX1 for cell growth and survival, the molecular mechanism of how PBX1 promotes tumorigenesis...
Takahiro Kawanabe et al.
Gene, 506(2), 301-309 (2012-07-17)
Difference in the level of expression of genes is one of the factors contributing to plant phenotype. As well as being under genetic control, gene expression is regulated by epigenetic processes such as DNA methylation and histone modifications. We compared...
Elizabeth P Murchison et al.
Cell, 148(4), 780-791 (2012-02-22)
The Tasmanian devil (Sarcophilus harrisii), the largest marsupial carnivore, is endangered due to a transmissible facial cancer spread by direct transfer of living cancer cells through biting. Here we describe the sequencing, assembly, and annotation of the Tasmanian devil genome...
Y R Thorstenson et al.
Genome research, 8(8), 848-855 (1998-09-02)
An automated, inexpensive, easy-to-use, and reproducible technique for controlled, random DNA fragmentation has been developed. The technique is based on point-sink hydrodynamics that result when a DNA sample is forced through a small hole by a syringe pump. Commercially available...
Koichi Suzuki et al.
PloS one, 5(8), e12422-e12422 (2010-09-25)
Identification of pathogen DNA from archaeological human remains is a powerful tool in demonstrating that the infectious disease existed in the past. However, it is very difficult to detect trace amounts of DNA remnants attached to the human skeleton, especially...

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