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Fei Huang et al.
Molecules (Basel, Switzerland), 19(8), 12760-12776 (2014-08-21)
Dried litchi pulp has been used in traditional remedies in China for many years to treat various diseases, and the therapeutic activity has been, at least partly, attributed to the presence of bioactive polysaccharides. Polysaccharide-protein complexes from vacuum freeze-(VF), vacuum
Tong-Chao Su et al.
Scientific reports, 5, 17538-17538 (2015-12-10)
In this study, magnetic carbonaceous acids were synthesized by pyrolysis of the homogeneous mixtures of glucose and magnetic Fe3O4 nanoparticles, and subsequent sulfonation. The synthesis conditions were optimized to obtain a catalyst with both high acid density (0.75 mmol g(-1))
Lucas Ferreira Ribeiro et al.
Biotechnology for biofuels, 8, 118-118 (2015-08-19)
Product inhibition can reduce catalytic performance of enzymes used for biofuel production. Different mechanisms can cause this inhibition and, in most cases, the use of classical enzymology approach is not sufficient to overcome this problem. Here we have used a
Tian Xia et al.
Applied and environmental microbiology, 81(10), 3387-3394 (2015-03-10)
Escherichia coli that is unable to metabolize d-glucose (with knockouts in ptsG, manZ, and glk) accumulates a small amount of d-glucose (yield of about 0.01 g/g) during growth on the pentoses d-xylose or l-arabinose as a sole carbon source. Additional
Wellington Muchero et al.
BMC genomics, 16, 24-24 (2015-01-24)
QTL cloning for the discovery of genes underlying polygenic traits has historically been cumbersome in long-lived perennial plants like Populus. Linkage disequilibrium-based association mapping has been proposed as a cloning tool, and recent advances in high-throughput genotyping and whole-genome resequencing
Pengfei Sun et al.
Scientific reports, 5, 17465-17465 (2015-12-03)
In the present work, a novel bioflocculant, EPS-1, was prepared and used to flocculate the kaolin suspension and Microcystis aeruginosa. We focused on the characteristics and flocculation performance of EPS-1, especially with regard to its protein components. An important attribute
Chen Chen et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 969, 171-180 (2014-09-01)
One challenge facing the purification of therapeutic glycoproteins by affinity chromatography is creating ligands specific for the glycan moiety. Affinity chromatography of glycoproteins is currently conducted with immobilized lectins or boronates, although biomimetic ligands could present a more desirable option.
Hidetada Ogata et al.
The Journal of endocrinology, 222(2), 191-200 (2014-06-04)
Glucose-dependent insulinotropic polypeptide (GIP), a gut hormone secreted from intestinal K-cells, potentiates insulin secretion. Both K-cells and pancreatic β-cells are glucose-responsive and equipped with a similar glucose-sensing apparatus that includes glucokinase and an ATP-sensitive K(+) (KATP) channel comprising KIR6.2 and
Dorit Eberhardt et al.
AMB Express, 4(1), 85-85 (2015-08-13)
L-citrulline plays an important role in human health and nutrition and is an intermediate of the L-arginine biosynthetic pathway. L-citrulline is a by-product of L-arginine production by Corynebacterium glutamicum. In this study, C. glutamicum was engineered for overproduction of L-citrulline
Benjamin L Golomb et al.
Journal of bacteriology, 197(2), 371-381 (2014-11-12)
Lactic acid bacteria have been isolated from living, harvested, and fermented plant materials; however, the adaptations these bacteria possess for growth on plant tissues are largely unknown. In this study, we investigated plant habitat-specific traits of Lactococcus lactis during growth
Legesse Garedew et al.
BMC microbiology, 15, 100-100 (2015-05-13)
Listeriosis, mostly caused by Listeria monocytogenes species, has become a major concern to public health authorities due to its clinical severity and high mortality rate, particularly in high risk groups. Currently, there is limited information regarding the prevalence and antimicrobial
Nan Wang et al.
Carbohydrate polymers, 111, 47-55 (2014-07-20)
A neutral polysaccharide fraction (SMPA) prepared from the roots of Salvia miltiorrhiza by DEAE-cellulose and Sephadex G-100 chromatography was tested for its immune enhancing function in N-methyl-N'-nitro-nitrosoguanidine (MNNG)-induced gastric cancer rats by intragastric administration. SMPA (200mg/kg) treatment not only increased
Magali Boutard et al.
Nature communications, 7, 13783-13783 (2016-12-17)
Bacteria respond to their environment by regulating mRNA synthesis, often by altering the genomic sites at which RNA polymerase initiates transcription. Here, we investigate genome-wide changes in transcription start site (TSS) usage by Clostridium phytofermentans, a model bacterium for fermentation
Wenjian Guan et al.
Bioresource technology, 200, 713-721 (2015-11-13)
Paper mill sludge (PS), a solid waste from pulp and paper industry, was investigated as a feedstock for acetone-butanol-ethanol (ABE) production by simultaneous saccharification and fermentation (SSF). ABE fermentation of paper sludge by Clostridium acetobutylicum required partial removal of ash
Fátima Vargas et al.
Bioresource technology, 191, 263-270 (2015-05-23)
In the present work, the hydrothermal valorization of an abundant agricultural residue has been studied in order to look for high added value applications by means of hydrothermal pretreatment followed by fed-batch simultaneous saccharification and fermentation, to obtain oligomers and
Belisa B de Sales et al.
Biotechnology letters, 37(10), 1973-1982 (2015-06-20)
Since uptake of xylose limits its fermentation, we aimed to identify novel sugar transporters from Scheffersomyces stipitis that allow xylose uptake and fermentation by engineered Saccharomyces cerevisiae. An hxt-null S. cerevisiae strain, lacking the major hexose transporters (hxt1Δ-hxt7Δ and gal2Δ)
Rongjun He et al.
International journal of biological macromolecules, 78, 56-61 (2015-04-04)
Three polysaccharides (SAP30, SAP60 and SAP80) were separated from the body of Edwardsia sipunculoides by tissue homogenate and papain hydrolysis. Total soluble sugar contents, monosaccharide compositions, antioxidant and antitumor activities in vitro of those polysaccharides were investigated, respectively. Results showed
Joseph A Rollin et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(16), 4964-4969 (2015-04-08)
The use of hydrogen (H2) as a fuel offers enhanced energy conversion efficiency and tremendous potential to decrease greenhouse gas emissions, but producing it in a distributed, carbon-neutral, low-cost manner requires new technologies. Herein we demonstrate the complete conversion of
Astrid Rohwedder et al.
Chemical senses, 37(8), 711-721 (2012-06-15)
Gustatory stimuli allow an organism not only to orient in its environment toward energy-rich food sources to maintain nutrition but also to avoid unpleasant or even poisonous substrates. For both mammals and insects, sugars-perceived as "sweet"-potentially predict nutritional benefit. Interestingly
Stephen R Hughes et al.
Journal of laboratory automation, 20(6), 621-635 (2015-02-28)
A yeast artificial chromosome (YAC) containing a multigene cassette for expression of enzymes that enhance xylose utilization (xylose isomerase [XI] and xylulokinase [XKS]) was constructed and transformed into Saccharomyces cerevisiae to demonstrate feasibility as a stable protein expression system in
Huiling Li et al.
Bioresource technology, 176, 242-248 (2014-12-03)
The conversion of xylose, water-insoluble hemicelluloses (WIH) and water-soluble fraction (WSF) of corncob to furfural was performed using montmorillonite with tin ions (Sn-MMT) containing double acid sites as a solid acid catalyst. The co-existence of Lewis acids and Brønsted acids
Jin Qi et al.
Chemical & pharmaceutical bulletin, 63(3), 187-194 (2015-03-12)
Two new steroidal glycosides (1 and 2), together with 15 known compounds (3-17) were isolated from the fibrous roots of Ophiopogon japonicus, and three new steroidal glycosides (18-20), together with 14 known compounds (21-34) were isolated from the fibrous roots
Huijun Wang et al.
Carbohydrate polymers, 124, 98-108 (2015-04-04)
A water-soluble polysaccharide, 7WA, with an average molecular mass of 7.1×10(4)Da, was isolated from the leaves of green tea. Monosaccharide composition analysis indicated that 7WA mainly contained Arabinose and Galactose in the molar ratio of 1.0:0.96. By using the methods
Meng Li et al.
Bioresource technology, 167, 14-23 (2014-06-27)
Sweet sorghum has been regarded as a typical species for rich soluble-sugar and high lignocellulose residues, but their effects on biomass digestibility remain unclear. In this study, we examined total 63 representative sweet sorghum accessions that displayed a varied sugar
Alexandre F Somera et al.
Applied and environmental microbiology, 81(13), 4525-4535 (2015-04-26)
Leaf-cutter ants use plant matter to culture the obligate mutualistic basidiomycete Leucoagaricus gongylophorus. This fungus mediates ant nutrition on plant resources. Furthermore, other microbes living in the fungus garden might also contribute to plant digestion. The fungus garden comprises a
Philippe Debeire et al.
FEMS microbiology letters, 355(2), 116-123 (2014-05-16)
Fusarium graminearum was grown on four lignocellulosic substrates (corn cobs, wheat bran, hop cell walls, and birchwood) and glucose as the sole carbon source. Proteomic studies performed on the resulting enzymatic cocktails highlighted a great diversity in the number and
Azam Fatahi Sadeghabadi et al.
Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences, 19(Suppl 1), S51-S55 (2014-07-09)
Antibiotic resistance of enteric pathogens particularly Shigella species, is a critical world-wide problem and monitoring their resistant pattern is essential, because the choice of antibiotics is absolutely dependent on regional antibiotic susceptibility patterns. During summer 2013, an unusual increase in
Leya Thomas et al.
Indian journal of experimental biology, 53(6), 356-363 (2015-07-15)
Here, we described the production of a cellulase-free alkaline xylanase from Bacillus pumilus MTCC 5015 by submerged fermentation and its application in biobleaching. Various process parameters affecting xylanase production by B. pumilus were optimized by adopting a Plackett-Burman design (PBD)
Yang Xing et al.
Bioresource technology, 193, 401-407 (2015-07-08)
This study reports four schemes to pretreat wet furfural residues (FRs) with sodium bisulfite for production of fermentable sugar. The results showed that non-detoxified FRs (pH 2-3) had great potential to lower the cost of bioconversion. The optimal process was
Meigui Huang et al.
Journal of food science and technology, 52(2), 858-866 (2015-02-20)
A novel gradient temperature-elevating Maillard reaction was developed to effectively produce light-colored MRPs. The main purpose of the present study was to compare the color, taste characteristic, non-volatile compounds, volatile compounds and antioxidant activity of MRPs prepared by the novel
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