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C Graham Clark et al.
Current protocols in microbiology, 43, 20A-20A (2016-11-20)
Blastocystis is an intestinal parasite that is very easily isolated in culture from fresh stool samples. In fact, the parasite grows so readily in culture that short-term in vitro culture is sometimes used as a diagnostic tool in the absence
Antonella Denise Losinno et al.
Autophagy, 17(2), 439-456 (2020-01-28)
Cruzipain, the major cysteine protease of the pathogenic protozoa Trypanosoma cruzi, is an important virulence factor that plays a key role in the parasite nutrition, differentiation and host cell infection. Cruzipain is synthesized as a zymogen, matured, and delivered to
Melanie Hornschuh et al.
Acta biomaterialia, 106, 376-386 (2020-02-19)
Immobilized polycationic substances on biomaterial surfaces kill adhering bacteria upon contact and are considered a promising non-antibiotic alternative. Unfortunately, there is no generally accepted in vitro method for quantitatively evaluating the antibacterial efficacy of contact-active non-leachable antimicrobial surfaces. Moreover, guidelines
Benjamin A Adducci et al.
Biosensors & bioelectronics, 78, 160-166 (2015-11-26)
New methods and technology are needed to quickly and accurately detect potential biological warfare agents, such as Bacillus anthracis, causal agent of anthrax in humans and animals. Here, we report the detection of a simulant of B. anthracis (B. globigii)
Juan Andres Imelio et al.
Bio-protocol, 7(16), e2510-e2510 (2017-08-20)
We have developed protocols to generate site-specific variants of the histidine-kinase DesK and its cognate response regulator DesR, conducive to trapping different signaling states of the proteins. Co-expression of both partners in E. coli, ensuring an excess of the regulator
Fatemeh Kiaeian Moosavi et al.
Archives of insect biochemistry and physiology, 103(4), e21655-e21655 (2020-01-19)
Some studies have highlighted benefits for Lobesia botrana by adding Botrytis cinerea mycelium to an artificial larval diet and have suggested a mutualistic relationship between the two organisms on grapevine, hypothesizing that fungal sterols were the nutritional factor involved. Because
A Adekunte et al.
International journal of food microbiology, 137(1), 116-120 (2009-11-13)
Power ultrasound is recognised as a potential non thermal technique to inactivate microorganisms pertinent to fruit juices. In this study, the effect of sonication on the resistance of yeast (Pichia fermentans) in tomato juice was investigated. Tomato juice samples were
Jing Li et al.
Bio-protocol, 7(15), e2434-e2434 (2017-08-05)
Streptococcus pneumoniae (pneumococcus) is an important human pathogen that causes pneumonia, meningitis, sepsis, and otitis media. This bacterium normally resides in the nasopharynx as a commensal, but sometimes disseminates to sterile sites of humans and causes local or systemic inflammation.
João Pedro Amorim et al.
STAR protocols, 1(3), 100208-100208 (2020-12-31)
Since its first application for site-directed mutagenesis, the CRISPR-Cas9 system has revolutionized genome engineering. Here, we present a validated workflow for the generation of targeted genomic deletions in zebrafish, including the design, cloning, and synthesis of single-guide RNAs and Cas9
Mohammed A Khedr et al.
Scientific reports, 8(1), 8565-8565 (2018-06-06)
Fungal infections are spreading widely worldwide, and the types of treatment are limited due to the lack of diverse therapeutic agents and their associated side effects and toxicity. The discovery of new antifungal classes is vital and critical. We discovered
Kuo-Fu Tseng et al.
Current biology : CB, 30(18), 3664-3671 (2020-08-01)
Kinesin-14s are microtubule-based motor proteins that play important roles in mitotic spindle assembly [1]. Ncd-type kinesin-14s are a subset of kinesin-14 motors that exist as homodimers with an N-terminal microtubule-binding tail, a coiled-coil central stalk (central stalk), a neck, and
Pascal Chatonnet et al.
Journal of agricultural and food chemistry, 58(23), 12481-12490 (2010-11-10)
This study identifies a previously isolated bacterium as Rhizobium excellensis, a new species of proteobacteria able to form a large quantity of 2-methoxy-3,5-dimethylpyrazine (MDMP). R. excellensis actively synthesizes MDMP from L-alanine and L-leucine and, to a lesser extent, from L-phenylalanine
Olena P Ishchuk et al.
Antibiotics (Basel, Switzerland), 9(1) (2020-01-08)
The opportunistic human fungal pathogen Candida albicans relies on cell morphological transitions to develop biofilm and invade the host. In the current study, we developed new regulatory molecules, which inhibit the morphological transition of C. albicans from yeast-form cells to
Sara Hooshangi et al.
PLoS computational biology, 7(9), e1002172-e1002172 (2011-10-08)
Quorum sensing (QS) enables bacterial multicellularity and selective advantage for communicating populations. While genetic "switching" phenomena are a common feature, their mechanistic underpinnings have remained elusive. The interplay between circuit components and their regulation are intertwined and embedded. Observable phenotypes
Cecilia Arahí Prato et al.
STAR protocols, 1(3), 100204-100204 (2020-12-31)
Galectins are soluble lectins that participate in many physiological and pathological functions. Since they can act extracellularly, the use of the recombinant protein is a recurrent strategy for studying their biological functions. Here, we provide a general protocol for the
Lukas Schertel et al.
Journal of the Royal Society, Interface, 17(166), 20200196-20200196 (2020-05-21)
Vivid colours found in living organisms are often the result of scattering from hierarchical nanostructures, where the interplay between order and disorder in their packing defines visual appearance. In the case of Flavobacterium IR1, the complex arrangement of the cells
Barbara Pretzner et al.
Bioengineering (Basel, Switzerland), 8(11) (2021-11-26)
Simplicity renders shake flasks ideal for strain selection and substrate optimization in biotechnology. Uncertainty during initial experiments may, however, cause adverse growth conditions and mislead conclusions. Using growth models for online predictions of future biomass (BM) and the arrival of
Yimiao Qu et al.
STAR protocols, 1(2), 100082-100082 (2020-10-29)
The biological phenotype is affected by the level of gene expression. Here, we provide a step-by-step protocol for precisely titrating and quantitatively observing the target gene expression level in budding yeast by manipulating its copy number in the genome. Using
Aude Cerutti et al.
Bio-protocol, 7(20), e2451-e2451 (2017-10-20)
The present protocol to visualize living bacteria at the pore level of cauliflower hydathodes is simple and trained users in confocal microscopy can execute it successfully. It can be easily adapted to capture images with other plant-microorganism interactions at the
Abhilash Padavannil et al.
Bio-protocol, 10(10), e3625-e3625 (2021-03-05)
Histones constitute the protein components of nucleosomes. Despite their small sizes, histones do not diffuse through the nuclear pore complex. Instead, they are transported to the nucleus by importins, either alone or in complex with histone chaperones. Determining the molecular
Mujeeb Shittu et al.
Methods and protocols, 4(1) (2021-04-04)
RNA in situ hybridization (ISH) is used to visualize spatio-temporal gene expression patterns with broad applications in biology and biomedicine. Here we provide a protocol for mRNA ISH in developing pupal wings and abdomens for model and non-model Drosophila species.
Cynthia Vanesa Rivero et al.
Frontiers in cellular and infection microbiology, 11, 657257-657257 (2021-09-04)
T. cruzi, the causal agent of Chagas disease, is a parasite able to infect different types of host cells and to persist chronically in the tissues of human and animal hosts. These qualities and the lack of an effective treatment
Samriddha Ray et al.
The Journal of cell biology, 190(5), 793-805 (2010-09-02)
The mechanisms that regulate cytoskeletal remodeling during the transition between mitosis and interphase are poorly understood. In fission yeast the MOR pathway promotes actin polarization to cell tips in interphase, whereas the SIN signaling pathway drives actomyosin ring assembly and
Amit Srivastava et al.
Bio-protocol, 10(4), e3528-e3528 (2021-03-04)
Anabaena sp. PCC 7120 (hereafter Anabaena) is a model cyanobacterium to study nitrogen fixation, cellular differentiation and several other key biological functions that are analogous in plants. As with any other organism, many genes in Anabaena encode an essential life
Daeun Moon et al.
Molecular medicine reports, 20(4), 3933-3941 (2019-08-23)
The microbiome has recently attracted research interest in a variety of subjects, including cancer. In the present study, it was determined that reinforced clostridium media (RCM) for microbiome culture, exerts antitumor effects on renal cell carcinoma cells when compared to
Heidi M Blank et al.
Molecular biology of the cell, 31(10), 1069-1084 (2020-03-05)
Establishing the pattern of abundance of molecules of interest during cell division has been a long-standing goal of cell cycle studies. Here, for the first time in any system, we present experiment-matched datasets of the levels of RNAs, proteins, metabolites
Luciana M Katiki et al.
Veterinary parasitology, 182(2-4), 264-268 (2011-06-18)
The most challenging obstacles to testing products for their anthelmintic activity are: (1) establishing a suitable nematode in vitro assay that can evaluate potential product use against a parasitic nematode of interest and (2) preparation of extracts that can be
Colin Charnock
Microorganisms, 9(1) (2021-01-07)
Plastic pollution has become one of the most critical environmental issues, as rapidly increasing production, compounded by persistence of plastic wastes in the environment, are outpacing efforts to keep ecosystems plastic-free. A switch to plastics more amenable to microbial attack
Joshua E Mayfield et al.
Bio-protocol, 10(12), e3648-e3648 (2021-03-05)
Eukaryotic RNA polymerase II transcribes all protein-coding mRNAs and is highly regulated. A key mechanism directing RNA polymerase II and facilitating the co-transcriptional processing of mRNAs is the phosphorylation of its highly repetitive carboxyl-terminal domain (CTD) of its largest subunit
Daeun Moon et al.
Annals of clinical and laboratory science, 50(1), 31-44 (2020-03-13)
The microbiome has recently become a key interest for cancer research. Anti-tumor effects of reinforced clostridium media (RCM) were investigated for all ingredients of RCM, which showed that yeast extract could be a candidate for this phenomenon. MTT assay, cell
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