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Laser desorption sample transfer for gas chromatography/mass spectrometry.

Rapid communications in mass spectrometry : RCM (2015-12-15)
Chinthaka A Seneviratne, Suman Ghorai, Kermit K Murray
ABSTRACT

Ambient mass spectrometry can detect small molecules directly, but complex mixtures can be a challenge. We have developed a method that incorporates small molecule separation based on laser desorption with capture on a solid-phase microextraction (SPME) fiber for injection into a gas chromatography/mass spectrometry (GC/MS) system. Samples on a metal target were desorbed by a 3 µm mid-infrared laser focused to a 250 µm spot and 1.2 mJ pulse energy. The desorbed material was aspirated into a metal tube suspended 1 mm above the laser spot and captured on a SPME fiber. The collected material was injected into a GC/MS instrument for analysis. We have developed a versatile approach for ambient laser desorption sampling onto SPME for GC/MS analysis. The performance of the laser desorption SPME capture GC/MS system was demonstrated for small molecule standards, a mixture of nitroaromatic explosives, and collected cigarette smoke. The utility of ambient laser desorption sampling onto SPME for GC/MS was demonstrated. The performance of the method was evaluated by preparing calibration standards of caffeine over a range from 200 to 1000 ng. Laser desorption ambient sampling of complex mixtures was accomplished using SPME GC/MS.

MATERIALS
Product Number
Brand
Product Description

Supelco
SPME fiber assortment kit 5, needle size 23 ga, for use with autosampler
Supelco
SPME StableFlex fiber assortment kit, for use with autosampler, needle size 24 ga
Supelco
SPME fiber assortment kit 2, for use with manual holder, needle size 24 ga
Supelco
SPME fiber assortment kit 4, for use with autosampler, needle size 23 ga
Supelco
SPME fiber assortment kit 2, for use with autosampler, needle size 23 ga
Supelco
SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), df 65 μm (PDMS/DVB), needle size 23 ga, PDMS/DVB StableFlex, for use with autosampler
Supelco
SPME StableFlex fiber assortment kit, needle size 23 ga, for use with autosampler
Supelco
SPME fiber assortment kit 1, for use with autosampler, needle size 23 ga
Supelco
SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), df 65 μm (PDMS/DVB), for use with autosampler, needle size 23 ga, metal alloy fiber
Supelco
SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), df 65 μm (PDMS/DVB), needle size 24 ga, StableFlex, for use with autosampler
Supelco
SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), df 65 μm (PDMS/DVB), needle size 24 ga, for use with manual holder
Supelco
SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), df 65 μm (PDMS/DVB), needle size 24 ga, StableFlex, for use with manual holder
Supelco
SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), fused silica fiber, df 65 μm (PDMS/DVB), for use with manual holder, needle size 23 ga
Supelco
SPME StableFlex fiber assortment kit, for use with manual holder
Supelco
SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), df 65 μm (PDMS/DVB), needle size 24 ga, for use with autosampler
Supelco
SPME fiber assortment kit 1, for use with manual holder, needle size 24 ga
Supelco
SPME fiber assortment kit 4, for use with manual holder, size 24 ga
Supelco
SPME fiber assortment kit 1, for use with autosampler, needle size 24 ga
Supelco
SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), df 65 μm (PDMS/DVB), for use with autosampler, needle size 23 ga
Supelco
SPME fiber assortment kit 4, for use with autosampler, size 24 ga
Supelco
SPME fiber assortment kit 2, for use with autosampler, size 24 ga
Supelco
SPME fiber assembly, Carbowax-Polyethylene Glycol (PEG) Coating, needle size 23 ga, df 60 μm (PEG), for use with autosampler
Supelco
SPME fiber assembly, Carbowax-Polyethylene Glycol (PEG) Coating, needle size 23 ga, df 60 μm (PEG), metal alloy fiber, for use with manual holder
Supelco
IonSense® PDMS/DVB SPE-it Tips, pkg of 96 ea
Supelco
SPME LC Tips, C18, tray of 96 ea
Supelco
IonSense® C18 SPE-it Tips, pkg of 96 ea
Supelco
SPME LC Tips, PDMS/DVB, pkg of 96 ea
Supelco
SPME fiber assembly Polydimethylsiloxane (PDMS), metal alloy fiber, df 100 μm (PDMS), needle size 23 ga, for use with autosampler
Supelco
SPME fiber assembly Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), needle size 23 ga, StableFlex, for use with autosampler
Supelco
SPME fiber assembly Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), needle size 23 ga, StableFlex, for use with manual holder or autosampler, fiber L 2 cm