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  • Fiber content of diet affects exhaled breath volatiles in fasting and postprandial state in a pilot crossover study.

Fiber content of diet affects exhaled breath volatiles in fasting and postprandial state in a pilot crossover study.

Nutrition research (New York, N.Y.) (2016-05-18)
Kaisa J Raninen, Jenni E Lappi, Maria L Mukkala, Tomi-Pekka Tuomainen, Hannu M Mykkänen, Kaisa S Poutanen, Olavi J Raatikainen
ABSTRACT

Our pilot study examined the potential of exhaled breath analysis in studying the metabolic effects of dietary fiber (DF). We hypothesized that a high-fiber diet (HFD) containing whole grain rye changes volatile organic compound (VOC) levels in exhaled breath and that consuming a single meal affects these levels. Seven healthy men followed a week-long low-fiber diet (17 g/d) and HFD (44 g/d) in a randomized crossover design. A test meal containing 50 g of the available carbohydrates from wheat bread was served as breakfast after each week. Alveolar exhaled breath samples were analyzed at fasting state and 30, 60, and 120 minutes after this meal parallel to plasma glucose, insulin, and serum lipids. We used solid-phase microextraction and gas chromatography-mass spectrometry for detecting changes in 15 VOCs. These VOCs were acetone, ethanol, 1-propanol, 2-propanol, 1-butanol, acetic acid, propionic acid, butyric acid, valeric acid, isovaleric acid, 2-methylbutyric acid, hexanoic acid, acetoin, diacetyl, and phenol. Exhaled breath 2-methylbutyric acid in the fasting state and 1-propanol at 120 minutes decreased (P = .091 for both) after an HFD. Ingestion of the test meal increased ethanol, 1-propanol, acetoin, propionic acid, and butyric acid levels while reducing acetone, 1-butanol, diacetyl, and phenol levels. Both DF diet content and having a single meal affected breathVOCs. Exploring exhaled breath further could help to develop tools for monitoring the metabolic effects of DF.

MATERIALS
Product Number
Brand
Product Description

Supelco
SPME fiber assortment kit 5, needle size 23 ga, for use with autosampler
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SPME StableFlex fiber assortment kit, for use with autosampler, needle size 24 ga
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SPME fiber assortment kit 2, for use with manual holder, needle size 24 ga
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SPME fiber assortment kit 4, for use with autosampler, needle size 23 ga
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SPME fiber assortment kit 2, for use with autosampler, needle size 23 ga
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SPME fiber assortment kit 1, for use with autosampler, needle size 23 ga
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SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), df 65 μm (PDMS/DVB), for use with autosampler, needle size 23 ga, metal alloy fiber
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SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), df 65 μm (PDMS/DVB), needle size 24 ga, StableFlex, for use with autosampler
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SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), df 65 μm (PDMS/DVB), needle size 24 ga, for use with manual holder
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SPME Fiber Assembly Polydimethylsiloxane/Divinylbenzene (PDMS/DVB), df 65 μm (PDMS/DVB), needle size 24 ga, for use with autosampler
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SPME fiber assortment kit 1, for use with manual holder, needle size 24 ga
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SPME fiber assortment kit 4, for use with manual holder, size 24 ga
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SPME fiber assortment kit 4, for use with autosampler, size 24 ga
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