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Costamere protein expression and tissue composition of rotator cuff muscle after tendon release in sheep.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society (2017-06-03)
Severin Ruoss, Christoph B Möhl, Mario C Benn, Brigitte von Rechenberg, Karl Wieser, Dominik C Meyer, Christian Gerber, Martin Flück

Previous studies suggested that degradation of contractile tissue requires cleavage of the costamere, a structural protein complex that holds sarcomeres in place. This study examined if costamere turnover is affected by a rotator cuff tear in a previously established ovine model. We found the activity of focal adhesion kinase (FAK), a main regulator of costamere turnover, was unchanged at 2 weeks but decreased by 27% 16 weeks after surgical release of the infraspinatus tendon. This was accompanied by cleavage of the costamere protein talin into a 190 kDa fragment while full length talin remained unchanged. At 2 weeks after tendon release, muscle volume decreased by 17 cm3 from an initial 185 cm3 , the fatty tissue volume was halved, and the contractile tissue volume remained unchanged. After 16 weeks, the muscle volume decreased by 36 cm3 , contractile tissue was quantitatively lost, and the fat content increased by 184%. Nandrolone administration mitigated the loss of contractile tissue by 26% and prevented fat accumulation, alterations in FAK activity, and talin cleavage. Taken together, these findings imply that muscle remodeling after tendon release occurs in two stages. The early decrease of muscle volume is associated with reduction of fat; while, the second stage is characterized by substantial loss of contractile tissue accompanied by massive fat accumulation. Regulation of costamere turnover is associated with the loss of contractile tissue and seems to be impacted by nandrolone treatment. Clinically, the costamere may represent a potential intervention target to mitigate muscle loss after a rotator cuff tear. © 2017 The Authors. Journal of Orthopaedic Research® published by Wiley Periodicals, Inc. on behalf of the Orthopaedic Research Society. J Orthop Res 36:272-281, 2018.

Product Number
Product Description

Monoclonal Anti-Myosin (Skeletal, Fast) antibody produced in mouse, clone MY-32, ascites fluid
Anti-Mouse IgG (Fab specific)–Peroxidase antibody produced in goat, affinity isolated antibody, buffered aqueous solution
Monoclonal Anti-Talin antibody produced in mouse, clone 8d4, ascites fluid
Anti-Myosin Antibody, slow muscle, clone NOQ7.5.4D, clone NOQ7.5.4D, Chemicon®, from mouse