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Chromatin Accessibility Landscape in Human Early Embryos and Its Association with Evolution.

Cell (2018-03-13)
Lei Gao, Keliang Wu, Zhenbo Liu, Xuelong Yao, Shenli Yuan, Wenrong Tao, Lizhi Yi, Guanling Yu, Zhenzhen Hou, Dongdong Fan, Yong Tian, Jianqiao Liu, Zi-Jiang Chen, Jiang Liu
ABSTRACT

The dynamics of the chromatin regulatory landscape during human early embryogenesis remains unknown. Using DNase I hypersensitive site (DHS) sequencing, we report that the chromatin accessibility landscape is gradually established during human early embryogenesis. Interestingly, the DHSs with OCT4 binding motifs are enriched at the timing of zygotic genome activation (ZGA) in humans, but not in mice. Consistently, OCT4 contributes to ZGA in humans, but not in mice. We further find that lower CpG promoters usually establish DHSs at later stages. Similarly, younger genes tend to establish promoter DHSs and are expressed at later embryonic stages, while older genes exhibit these features at earlier stages. Moreover, our data show that human active transposons SVA and HERV-K harbor DHSs and are highly expressed in early embryos, but not in differentiated tissues. In summary, our data provide an evolutionary developmental view for understanding the regulation of gene and transposon expression.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Mineral oil, BioReagent, for molecular biology, light oil
Sigma-Aldrich
M2 medium, With HEPES, without penicillin and streptomycin, liquid, sterile-filtered, suitable for mouse embryo cell culture
Sigma-Aldrich
Tyrode′s Solution, Acidic, liquid, sterile-filtered, suitable for mouse embryo cell culture