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Quantitation and Confirmation of Chloramphenicol, Florfenicol, and Nitrofuran Metabolites in Honey Using LC-MS/MS.

Journal of AOAC International (2017-12-01)
Brian T Veach, Renea Anglin, Thilak K Mudalige, Paula J Barnes
ABSTRACT

This paper describes a rapid and robust method utilizing a single liquid-liquid extraction for the quantitation and confirmation of chloramphenicol, florfenicol, and nitrofuran metabolites in honey. This methodology combines two previous extraction methods into a single extraction procedure and utilizes matrix-matched calibration standards and stable isotopically labeled standards to improve quantitation. The combined extraction procedure reduces the average extraction time by >50% when compared with previously used procedures. The drug residues were determined using two separate LC-tandem MS conditions. Validation of all the analytes was performed, with average quantitation ranging from 92 to 105% for all analytes and the RSDs for all analytes being ≤12%.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
2-Nitrofuran, 97%
Sigma-Aldrich
Furaltadone

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