• Home
  • Search Results
  • Placental miR-340 mediates vulnerability to activity based anorexia in mice.

Placental miR-340 mediates vulnerability to activity based anorexia in mice.

Nature communications (2018-04-25)
Mariana Schroeder, Mira Jakovcevski, Tamar Polacheck, Yonat Drori, Alessia Luoni, Simone Röh, Jonas Zaugg, Shifra Ben-Dor, Christiane Albrecht, Alon Chen
ABSTRACT

Anorexia nervosa (AN) is a devastating eating disorder characterized by self-starvation that mainly affects women. Its etiology is unknown, which impedes successful treatment options leading to a limited chance of full recovery. Here, we show that gestation is a vulnerable window that can influence the predisposition to AN. By screening placental microRNA expression of naive and prenatally stressed (PNS) fetuses and assessing vulnerability to activity-based anorexia (ABA), we identify miR-340 as a sexually dimorphic regulator involved in prenatal programming of ABA. PNS caused gene-body hypermethylation of placental miR-340, which is associated with reduced miR-340 expression and increased protein levels of several target transcripts, GR, Cry2 and H3F3b. MiR-340 is linked to the expression of several nutrient transporters both in mice and human placentas. Using placenta-specific lentiviral transgenes and embryo transfer, we demonstrate the key role miR-340 plays in the mechanism involved in early life programming of ABA.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
M2 medium, With HEPES, without penicillin and streptomycin, liquid, sterile-filtered, suitable for mouse embryo cell culture
Sigma-Aldrich
Mineral oil, light oil, BioReagent, suitable for mouse embryo cell culture
Sigma-Aldrich
M16 Medium, With sodium bicarbonate and lactic acid, without penicillin and streptomycin, liquid, sterile-filtered
Sigma-Aldrich
Monoclonal Anti-H3F3B antibody produced in mouse, clone 2D7-H1, purified immunoglobulin, buffered aqueous solution