MilliporeSigma
  • Home
  • Search Results
  • TGF-β1 acts as mediator in fluoride-induced autophagy in the mouse osteoblast cells.

TGF-β1 acts as mediator in fluoride-induced autophagy in the mouse osteoblast cells.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (2018-03-06)
Yangfei Zhao, Yanyan Li, Yufeng Gao, Mengke Yuan, Ram Kumar Manthari, Jinming Wang, Jundong Wang
ABSTRACT

It is well known that excess fluoride intake can result in fluorosis, which is a serious public health problem. TGF-β1 affects a wide variety of cellular activities and plays an important role in fluorosis. Recent literature proved that fluoride induces autophagy, however, the mechanism is still unclear, and the role of TGF-β1 in the fluoride-induced autophagy should be further illustrated. Therefore, in this study, plasmids and small interfering RNA (siRNA) were used to overexpress and silence the TGF-β1 expression in the osteoblasts cells. Our results demonstrated that sodium fluoride (NaF) (2.26 mg/L F-) exposure lead to a significant decrease in proliferation rate of mouse osteoblast cells in a dose-dependent manner with a concomitant increase in the expression levels of TGF-β1 and autophagic markers. Overexpression of TGF-β1 significantly increased the fluorescence intensity of MDCstained cells, the mRNA and protein expression levels of Beclin1 and LC3-II/I, with a concomitant decrease in p62. However, TGF-β1 silencing resulted in vice versa. In summary, we concluded that TGF-β1 plays a mediator role in NaF-induced autophagy of mouse osteoblast cells. In the future, TGF-β1-autophagy signaling network could be a new idea for the investigation of skeletal fluorosis.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Alizarin, Dye content 97 %
Sigma-Aldrich
MISSION® esiRNA, targeting human CHN1
Sigma-Aldrich
(Tyr[SO3H]27)Cholecystokinin fragment 26-33 Amide, ≥97% (HPLC), powder