• Home
  • Search Results
  • A protocol for preparing, characterizing and using three RNA-specific, live cell imaging probes: E36, E144 and F22.

A protocol for preparing, characterizing and using three RNA-specific, live cell imaging probes: E36, E144 and F22.

Nature protocols (2007-04-05)
Qian Li, Young-Tae Chang
ABSTRACT

This protocol outlines a methodology for the preparation and characterization of three RNA-specific fluorescent probes (E36, E144 and F22) and their use in live cell imaging. It describes a detailed procedure for their chemical synthesis and purification; serial product characterization and quality control tests, including measurements of their fluorescence properties in solution, measurement of RNA specificity and analysis of cellular toxicity; and live cell staining and counterstaining with Hoechst or DAPI. Preparation and application of these RNA imaging probes takes 1 week.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
2,4,6-Trimethoxybenzaldehyde, 98%
Sigma-Aldrich
6-Methoxyquinaldine, 97%
Sigma-Aldrich
1-Acetyl-3-indolecarboxaldehyde, 98%

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.