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  • A lead analysis based on amine functionalized bimodal mesoporous silica nanoparticles in human biological samples by ultrasound assisted-ionic liquid trap-micro solid phase extraction.

A lead analysis based on amine functionalized bimodal mesoporous silica nanoparticles in human biological samples by ultrasound assisted-ionic liquid trap-micro solid phase extraction.

Journal of pharmaceutical and biomedical analysis (2018-05-13)
Hamid Shirkhanloo, Sara Davari Ahranjani
ABSTRACT

A simple and applied method based on ultrasound assisted-Ionic liquid trap-micro solid phase extraction (USA-ILT-μSPE) was demonstrated for preconcentration/separation of lead (Pb) in human blood and urine samples. By proposed method, the amine-functionalized bimodal mesoporous silica nanoparticles (NH2-UVM7) as solid phase sorbent was used for lead analysis in human biological samples at pH ≈ 7.0. After shaking/centrifuging of sample, the Pb (II) was extracted with NH2-UVM7 without any chelating agents, trapped in ionic liquid phase and settled down in conical centrifuge tube as a residual solution [Pb→:NH2-UVM7]. Finally, the lead ions was back extracted from residual solution and determined by atom trap flame atomic absorption spectrometry (AT-FAAS). Under the optimal conditions, the linear range (LR), limit of detection (LOD) and preconcentration factor (PF) were obtained (10-950 μg L-1; 2.0-210 μg L-1), (2.8 μg L-1; 0.6 μg L-1) and (19.8; 99.7) for human blood and urine samples, respectively (% RSD < 1, intra-day). The reusability and adsorption capacity of the sorbent were also obtained 24 and 213.8 mg g-1, respectively. Validation of methodology was confirmed by certified reference materials (NIST, CRM).

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
1-Ethyl-3-methylimidazolium hexafluorophosphate, ≥97.0% (HPLC)
Sigma-Aldrich
1-Hexyl-3-methylimidazolium hexafluorophosphate, ≥97.0% (HPLC)
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Hydrogen chloride solution, 4.0 M in dioxane
Milli-Q
Milli-Q® IQ 7000 Ultrapure Water Purification System, output: type 1 water (18.2 MΩ·cm), the most advanced Milli-Q® ultrapure (Type 1) water system that is intelligent, intuitive, and green.