• Home
  • Search Results
  • Stage-Specific Transcription Factors Drive Astrogliogenesis by Remodeling Gene Regulatory Landscapes.

Stage-Specific Transcription Factors Drive Astrogliogenesis by Remodeling Gene Regulatory Landscapes.

Cell stem cell (2018-10-06)
Neha Tiwari, Abhijeet Pataskar, Sophie Péron, Sudhir Thakurela, Sanjeeb Kumar Sahu, María Figueres-Oñate, Nicolás Marichal, Laura López-Mascaraque, Vijay K Tiwari, Benedikt Berninger

A broad molecular framework of how neural stem cells are specified toward astrocyte fate during brain development has proven elusive. Here we perform comprehensive and integrated transcriptomic and epigenomic analyses to delineate gene regulatory programs that drive the developmental trajectory from mouse embryonic stem cells to astrocytes. We report molecularly distinct phases of astrogliogenesis that exhibit stage- and lineage-specific transcriptomic and epigenetic signatures with unique primed and active chromatin regions, thereby revealing regulatory elements and transcriptional programs underlying astrocyte generation and maturation. By searching for transcription factors that function at these elements, we identified NFIA and ATF3 as drivers of astrocyte differentiation from neural precursor cells while RUNX2 promotes astrocyte maturation. These transcription factors facilitate stage-specific gene expression programs by switching the chromatin state of their target regulatory elements from primed to active. Altogether, these findings provide integrated insights into the genetic and epigenetic mechanisms steering the trajectory of astrogliogenesis.

Product Number
Product Description

Monoclonal ANTI-FLAG® M2 antibody produced in mouse, 1 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)
D-(+)-Glucose, ≥99.5% (GC)
Chloroform, contains 100-200 ppm amylenes as stabilizer, ≥99.5%
DAPI, for nucleic acid staining
Magnesium chloride, anhydrous, ≥98%
5-Bromo-2′-deoxyuridine, ≥99% (HPLC)
N-Lauroylsarcosine sodium salt, detergent for use in cell lysis
Anti-NeuN Antibody, clone A60, clone A60, Chemicon®, from mouse
Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody produced in mouse, clone G-A-5, ascites fluid
Anti-Olig-2 Antibody, Chemicon®, from rabbit
HEPES potassium salt, ≥99.5% (titration)
Monoclonal Anti-β-Tubulin III antibody produced in mouse, clone SDL.3D10, ascites fluid
Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid, BioXtra, ≥97 .0%
Anti-Doublecortin Antibody, serum, from guinea pig
Phenol solution, 100 μg/mL in acetonitrile, PESTANAL®, analytical standard

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon


Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.