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  • Development of a standardized subgrouping scheme for Legionella pneumophila serogroup 1 using monoclonal antibodies.

Development of a standardized subgrouping scheme for Legionella pneumophila serogroup 1 using monoclonal antibodies.

Journal of clinical microbiology (1986-04-01)
J R Joly, R M McKinney, J O Tobin, W F Bibb, I D Watkins, D Ramsay
ABSTRACT

A panel of monoclonal antibodies to Legionella pneumophila serogroup 1 and a subclassification scheme were developed in a collaborative project among three laboratories. The seven most useful monoclonal antibodies were selected from three previously developed panels on the basis of indirect fluorescent antibody patterns with 83 strains of L. pneumophila serogroup 1 that were obtained from widely distributed geographic locations. The isolates were divided into 10 major subgroups on the basis of reactivity patterns that can be readily reproduced in any laboratory and are not subject to major inconsistencies of interpretation of staining intensity. A standard protocol for the indirect fluorescent antibody procedure was also developed.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-L. pneumophila Serogroup 1 Antibody, clone Lp1 3, ascites fluid, clone Lp1 MAB 3, Chemicon®
Sigma-Aldrich
Anti-L. pneumophila Serogroup 1 Antibody, clone Lp1 1, ascites fluid, clone Lp1 MAB 1, Chemicon®