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Inexpensive purification of P450 reductase and other proteins using 2',5'-adenosine diphosphate agarose affinity columns.

Protein expression and purification (2001-06-05)
D Rock, D Rock, J P Jones
ABSTRACT

Two reductases, P450 oxidoreductase and P450Bm-3 reductase, were purified on a 2',5'-adenosine diphosphate solid support. Although the efficiency of these columns is well established, the cost of the resin and the eluting material 2'-adenosine can be prohibitive. Herein we show that the less costly 2',3'-adenosine monophosphate is an excellent eluting material.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Adenosine 2′,5′-diphosphate sodium salt, ≥95%