• Home
  • Search Results
  • On the mechanism of thrombin-induced angiogenesis: involvement of alphavbeta3-integrin.

On the mechanism of thrombin-induced angiogenesis: involvement of alphavbeta3-integrin.

American journal of physiology. Cell physiology (2002-10-10)
Nikos E Tsopanoglou, Paraskevi Andriopoulou, Michael E Maragoudakis
ABSTRACT

Thrombin has been reported to be a potent angiogenic factor both in vitro and in vivo, and many of the cellular effects of thrombin may contribute to activation of angiogenesis. In this report we show that thrombin-treatment of human endothelial cells increases mRNA and protein levels of alphavbeta3-integrin. This thrombin-mediated effect is specific, dose dependent, and requires the catalytic site of thrombin. In addition, thrombin interacts with alphavbeta3 as demonstrated by direct binding of alphavbeta3 protein to immobilized thrombin. This interaction of thrombin with alphavbeta3-integrin, which is an angiogenic marker in vascular tissue, is of functional significance. Immobilized thrombin promotes endothelial cells attachment, migration, and survival. Antibody to alphavbeta3 or a specific peptide antagonist to alphavbeta3 can abolish all these alphavbeta3-mediated effects. Furthermore, in the chick chorioallantoic membrane system, the antagonist peptide to alphavbeta3 diminishes both basal and the thrombin-induced angiogenesis. These results support the pivotal role of thrombin in activation of endothelial cells and angiogenesis and may be related to the clinical observation of neovascularization within thrombi.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-Integrin αVβ3 Antibody, clone LM609, clone LM609, Chemicon®, from mouse
Sigma-Aldrich
Goat Anti-Mouse IgG Antibody, Peroxidase Conjugated, H+L, 1 mg/mL (after reconstitution), Chemicon®
Sigma-Aldrich
Anti-Integrin β3 Antibody, clone PM6/13, clone PM6/13, Chemicon®, from mouse
Sigma-Aldrich
Human Integrin αVβ3 Protein, Triton X-100 Formulation
Sigma-Aldrich
Anti-Integrin αVβ3 Antibody, clone LM609, Alexa Fluor®488 conjugated, clone LM609, Chemicon®, from mouse