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  • Stable kinesin and dynein assemblies drive the axonal transport of mammalian prion protein vesicles.

Stable kinesin and dynein assemblies drive the axonal transport of mammalian prion protein vesicles.

Cell (2011-02-22)
Sandra E Encalada, Lukasz Szpankowski, Chun-hong Xia, Lawrence S B Goldstein
ABSTRACT

Kinesin and dynein are opposite-polarity microtubule motors that drive the tightly regulated transport of a variety of cargoes. Both motors can bind to cargo, but their overall composition on axonal vesicles and whether this composition directly modulates transport activity are unknown. Here we characterize the intracellular transport and steady-state motor subunit composition of mammalian prion protein (PrP(C)) vesicles. We identify Kinesin-1 and cytoplasmic dynein as major PrP(C) vesicle motor complexes and show that their activities are tightly coupled. Regulation of normal retrograde transport by Kinesin-1 is independent of dynein-vesicle attachment and requires the vesicle association of a complete Kinesin-1 heavy and light chain holoenzyme. Furthermore, motor subunits remain stably associated with stationary as well as with moving vesicles. Our data suggest a coordination model wherein PrP(C) vesicles maintain a stable population of associated motors whose activity is modulated by regulatory factors instead of by structural changes to motor-cargo associations.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
AXIS Axon Isolation Device, 450 µm, The AXIS Axon Isolation Devices have been shown to effectively isolate cell bodies from differentiating neurites for a variety of cell types.
Sigma-Aldrich
AXIS Axon Isolation Device, 450 µm, The AXIS Axon Isolation Devices have been shown to effectively isolate cell bodies from differentiating neurites for a variety of cell types.

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