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Modified aptamers enable quantitative sub-10-nm cellular DNA-PAINT imaging.

Nature methods (2018-08-22)
Sebastian Strauss, Philipp C Nickels, Maximilian T Strauss, Vilma Jimenez Sabinina, Jan Ellenberg, Jeffrey D Carter, Shashi Gupta, Nebojsa Janjic, Ralf Jungmann

Although current implementations of super-resolution microscopy are technically approaching true molecular-scale resolution, this has not translated to imaging of biological specimens, because of the large size of conventional affinity reagents. Here we introduce slow off-rate modified aptamers (SOMAmers) as small and specific labeling reagents for use with DNA points accumulation in nanoscale topography (DNA-PAINT). To demonstrate the achievable resolution, specificity, and multiplexing capability of SOMAmers, we labeled and imaged both transmembrane and intracellular targets in fixed and live cells.

Product Number
Product Description

Trypsin-EDTA solution, 0.25%, sterile-filtered, BioReagent, suitable for cell culture, 2.5 g porcine trypsin and 0.2 g EDTA • 4Na per liter of Hanks′ Balanced Salt Solution with phenol red
Diethylamine, ≥99.5%
Bovine Serum Albumin, cold ethanol fraction, pH 5.2, ≥96%
hEGF, EGF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Ammonium chloride, 99.998% trace metals basis
Dextran sulfate sodium salt from Leuconostoc spp., mol wt 6,500-10,000