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Modified aptamers enable quantitative sub-10-nm cellular DNA-PAINT imaging.

Nature methods (2018-08-22)
Sebastian Strauss, Philipp C Nickels, Maximilian T Strauss, Vilma Jimenez Sabinina, Jan Ellenberg, Jeffrey D Carter, Shashi Gupta, Nebojsa Janjic, Ralf Jungmann
ABSTRACT

Although current implementations of super-resolution microscopy are technically approaching true molecular-scale resolution, this has not translated to imaging of biological specimens, because of the large size of conventional affinity reagents. Here we introduce slow off-rate modified aptamers (SOMAmers) as small and specific labeling reagents for use with DNA points accumulation in nanoscale topography (DNA-PAINT). To demonstrate the achievable resolution, specificity, and multiplexing capability of SOMAmers, we labeled and imaged both transmembrane and intracellular targets in fixed and live cells.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Trypsin-EDTA solution, 0.25%, sterile-filtered, BioReagent, suitable for cell culture, 2.5 g porcine trypsin and 0.2 g EDTA • 4Na per liter of Hanks′ Balanced Salt Solution with phenol red
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Ammonium chloride, 99.998% trace metals basis
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