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Embedding live bacteria in porous hydrogel/ceramic nanocomposites for bioprocessing applications.

Bioprocess and biosystems engineering (2019-04-07)
Jessica Condi Mainardi, Kurosch Rezwan, Michael Maas
ABSTRACT

In this work, we present a biocompatible one-pot processing route for ceramic/hydrogel nanocomposites in which we embed live bacteria. In our approach, we fabricate a highly stable alginate hydrogel with minimal shrinkage, highly increased structural and mechanical stability, as well as excellent biocompatibility. The hydrogel was produced by ionotropic gelation and reinforced with alumina nanoparticles to form a porous 3D network. In these composite gels, the bacteria Escherichia coli and Bacillus subtilis were embedded. The immobilized bacteria showed high viability and similar metabolic activity as non-embedded cells. Even after repeated glucose consumption cycles, the material maintained high structural stability with stable metabolic activity of the immobilized bacteria. Storing the bionanocomposite for up to 60 days resulted in only minor loss of activity. Accordingly, this approach shows great potential for producing macroscopic bioactive materials for biotechnological processes.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Phosphate buffered saline, tablet
Sigma-Aldrich
LB Broth (Lennox), Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture.
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 25% in H2O, specially purified for use as an electron microscopy fixative
Sigma-Aldrich
Calcium chloride dihydrate, tested according to Ph. Eur.