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In vitro fermentation of O‑acetyl‑arabinoxylan from bamboo shavings by human colonic microbiota.

International journal of biological macromolecules (2018-12-07)
Juqing Huang, Qi Wang, Qingxian Xu, Ying Zhang, Bin Lin, Xuefang Guan, Lei Qian, Yi Zheng
ABSTRACT

BSH-1 is an O‑acetyl-arabinoxylan obtained from bamboo shavings. This study investigated its fermentation behavior by human colonic microbiota in vitro. Results showed that BSH-1 remarkably modulated the composition of human colonic microbiota, mainly by increasing the growth of potential beneficial genera (i.e. Bifidobacterium, Lactobacillus, Bacteroides, Prevotella_7, Parabacteroides) and by decreasing the growth of potential harmful genera (i.e. Fusobacterium, Lachnospiraceae_UCG-008, Bilophila and Desulfovibrio). BSH-1 significantly promoted the production of short-chain fatty acids, especially acetic, propionic and n-butyric acids. After 48 h fermentation, the concentration of n-butyric acid in BSH-1 fermentation culture was increased by 2.41 times compared to the blank. During fermentation, the activity of acetyl xylan esterase, arabinofuranosidase, xylanase and xylosidase was enhanced. Moreover, free arabinose, xylose, xylobiose, xylotriose, xylotetraose, xylopentaose and xylohexaose were detected. These results suggest that BSH-1 could potentially be a functional ingredient to improve gut health.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Propyl chloroformate, 98%
Sigma-Aldrich
Xylobiose, ≥90% (HPLC)