A genetically encodable cell-type-specific protein synthesis inhibitor.

Nature methods (2019-07-17)
Maximilian Heumüller, Caspar Glock, Vidhya Rangaraju, Anne Biever, Erin M Schuman

Chemical inhibitors have revealed requirements for protein synthesis that drive cellular plasticity. We developed a genetically encodable protein synthesis inhibitor (gePSI) to achieve cell-type-specific temporal control of protein synthesis. Controlled expression of the gePSI in neurons or glia resulted in rapid, potent and reversible cell-autonomous inhibition of protein synthesis. Moreover, gePSI expression in a single neuron blocked the structural plasticity induced by single-synapse stimulation.