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Resetting histone modifications during human parental-to-zygotic transition.

Science (New York, N.Y.) (2019-07-06)
Weikun Xia, Jiawei Xu, Guang Yu, Guidong Yao, Kai Xu, Xueshan Ma, Nan Zhang, Bofeng Liu, Tong Li, Zili Lin, Xia Chen, Lijia Li, Qiujun Wang, Dayuan Shi, Senlin Shi, Yile Zhang, Wenyan Song, Haixia Jin, Linli Hu, Zhiqin Bu, Yang Wang, Jie Na, Wei Xie, Ying-Pu Sun

Histone modifications regulate gene expression and development. To address how they are reprogrammed in human early development, we investigated key histone marks in human oocytes and early embryos. Unlike that in mouse oocytes, the permissive mark trimethylated histone H3 lysine 4 (H3K4me3) largely exhibits canonical patterns at promoters in human oocytes. After fertilization, prezygotic genome activation (pre-ZGA) embryos acquire permissive chromatin and widespread H3K4me3 in CpG-rich regulatory regions. By contrast, the repressive mark H3K27me3 undergoes global depletion. CpG-rich regulatory regions then resolve to either active or repressed states upon ZGA, followed by subsequent restoration of H3K27me3 at developmental genes. Finally, by combining chromatin and transcriptome maps, we revealed transcription circuitry and asymmetric H3K27me3 patterning during early lineage specification. Collectively, our data unveil a priming phase connecting human parental-to-zygotic epigenetic transition.

Product Number
Product Description

Triton X-100, laboratory grade
Triton X-100, for molecular biology
DAPI, for nucleic acid staining
Bovine Serum Albumin, chromatographically purified, New Zealand origin, low endotoxin, suitable for cell culture, pH 7, ≥98%
Polyvinyl alcohol mounting medium with DABCO®, antifading, pH 8.7