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  • Effect of the molecular weight of chitosan on its antifungal activity against Candida spp. in planktonic cells and biofilm.

Effect of the molecular weight of chitosan on its antifungal activity against Candida spp. in planktonic cells and biofilm.

Carbohydrate polymers (2018-05-29)
Lana Glerieide Silva Garcia, Glaucia Morgana de Melo Guedes, Maria Lucilene Queiroz da Silva, Débora Souza Collares Maia Castelo-Branco, José Júlio Costa Sidrim, Rossana de Aguiar Cordeiro, Marcos Fábio Gadelha Rocha, Rodrigo Silveira Vieira, Raimunda Sâmia Nogueira Brilhante
ABSTRACT

Difficulties in the treatment of Candida spp. invasive infections are usually related to the formation of biofilms. The aim of this study was to determine the effects of molecular weight (MW) of chitosan (using high (HMW), medium (MMW) and low (LMW) molecular weight chitosan) on Candida albicans, Candida tropicalis and Candida parapsilosis sensu stricto. The deacetylation degree (DD) and molecular weight M were measured by potentiometric titration and viscosimetry, respectively. The planktonic shape activity was quantified by broth microdilution, and the activity against biofilm was quantified by metabolic activity through XTT 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]- 2H-tetrazolium hydroxide and biomass formation (crystal violet). The influence of chitosan MW on the planktonic form of Candida spp. was strain dependent. Fungal growth decreased with increasing chitosan MW for C. tropicalis and C. parapsilosis, while chitosan MW did not modulate the effect for C. albicans. With regard to the formation of biofilms, in both the adhesion and mature phases, the biomass and metabolic activities of Candida spp. were reduced by about 70% and 80%, respectively for each phase.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Chitosan, low molecular weight
Sigma-Aldrich
Chitosan, medium molecular weight
Sigma-Aldrich
Chitosan, high molecular weight