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Methyl groups as NMR probes for biomolecular interactions.

Current opinion in structural biology (2015-09-26)
Silke Wiesner, Remco Sprangers
ABSTRACT

Intermolecular interactions are indispensible for biological function. Here we discuss how novel NMR techniques can provide unique insights into the assembly, dynamics and regulation of biomolecular complexes. We focus on applications that exploit the methyl TROSY effect and show that methodological advances and biological insights go hand in hand. We envision that future methyl TROSY applications will continue to provide unique information regarding intermolecular interactions, even for very large eukaryotic protein complexes that are often highly asymmetric.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
DLAM-MεVproS-13CH3 Methyl Labeling Kit
Sigma-Aldrich
DLAM-AβVproS-13CH3 Methyl Labeling Kit
Sigma-Aldrich
DLAM-Iδ1Tγ-13CH3 Methyl Labeling Kit
Sigma-Aldrich
PLAM-AβIδ1LVProSTγ-13CH3 Methyl Labeling Kit
Sigma-Aldrich
TLAM-δI1LVproS-13CH3 Methyl Labeling Kit
Sigma-Aldrich
DLAM-LVproS-13CHD2 Methyl Labeling Kit
Sigma-Aldrich
HLAM-AβIδ1MεLVproSTγ-13CH3 Methyl Labeling Kit
Sigma-Aldrich
SLAM-VproR-13CH3 Methyl Labeling Kit
Sigma-Aldrich
TLAM-Iδ1LVproR-U-13C Methyl Labeling Kit
Sigma-Aldrich
TLAM-Iδ1MεTγ-13CH3 Methyl Labeling Kit
Sigma-Aldrich
QLAM-AβIδ1LVproS-13CH3 Methyl Labeling Kit
Sigma-Aldrich
SLAM-Aβ-13CH3 Methyl Labeling Kit
Sigma-Aldrich
DLAM-LVproR-13CH3 Methyl Labeling Kit
Sigma-Aldrich
SLAM-Iγ2-13CH3 Methyl Labeling Kit
Sigma-Aldrich
DLAM-Iδ1VproS-13CH3 Methyl Labeling Kit
Sigma-Aldrich
DLAM-LVproR-13C4 Methyl Labeling Kit
Sigma-Aldrich
SLAM-VproS-13CH3 Methyl Labeling Kit
Sigma-Aldrich
SLAM-VproS-13CHD2 Methyl Labeling Kit
Sigma-Aldrich
QLAM-Iδ1TγLVproS-13CH3 Methyl Labeling Kit
Sigma-Aldrich
SLAM-Aβ-13CHD2 Methyl Labeling Kit
Sigma-Aldrich
DLAM-LVproS-13CH3 Methyl Labeling Kit