MilliporeSigma
  • Home
  • Search Results
  • HISTONE DEACETYLASE 9 Functions with Polycomb Silencing to Repress FLOWERING LOCUS C Expression.

HISTONE DEACETYLASE 9 Functions with Polycomb Silencing to Repress FLOWERING LOCUS C Expression.

Plant physiology (2019-10-24)
Xiaolin Zeng, Zheng Gao, Chuan Jiang, Yupeng Yang, Renyi Liu, Yuehui He
ABSTRACT

Polycomb repressive complex 2 (PRC2) catalyzes repressive histone 3 Lys-27 trimethylation (H3K27me3) to mediate genome-wide transcriptional repression in plants and animals. PRC2 controls various developmental processes in plants and plays a critical role in the developmental transition to flowering. FLOWERING LOCUS C (FLC), first identified in Arabidopsis (Arabidopsis thaliana), is a potent floral repressor in crucifers and some other plants that is subjected to complex regulation. Here, we show that HISTONE DEACETYLASE 9 (HDA9)-mediated H3K27 deacetylation is required for PRC2-mediated H3K27me3 in Arabidopsis. We further demonstrate that through physical association with the epigenome readers VP1/ABI3-LIKE 1 (VAL1) and VAL2, which recognize a cis-regulatory element at the FLC locus, HDA9 and PRC2 function in concert to mediate H3K27 deacetylation and subsequent trimethylation at this residue. This leads to FLC repression in the rapid-cycling Arabidopsis accessions. Our study uncovers roles for HDA9 in PRC2-mediated H3K27me3, FLC repression, and flowering-time regulation.

MATERIALS
Product Number
Brand
Product Description

Roche
Anti-HA-Peroxidase, High Affinity, from rat IgG1
Sigma-Aldrich
Anti-acetyl-Histone H4 (Lys5) Antibody, serum, from rabbit
Sigma-Aldrich
Anti-trimethyl-Histone H3 (Lys27) Antibody, Upstate®, from rabbit
Sigma-Aldrich
Anti-acetyl-Histone H3 (Lys9/18) Antibody, serum, Upstate®
Sigma-Aldrich
Anti-acetyl-Histone H3 (Lys23) Antibody, serum, Upstate®
Sigma-Aldrich
Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody produced in mouse, clone M2, purified immunoglobulin, buffered aqueous glycerol solution
Millipore
EZview Red ANTI-FLAG® M2 Affinity Gel, clone M2
Sigma-Aldrich
Anti-RLN3 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Anti-acetyl-Histone H3 (Lys14) Antibody, serum, Upstate®
Sigma-Aldrich
Anti-Histone H3 Antibody, CT, pan, serum, Upstate®