• Magnetic resonance microscopy and correlative histopathology of the infarcted heart.

Magnetic resonance microscopy and correlative histopathology of the infarcted heart.

Scientific reports (2019-12-29)
Itziar Perez-Terol, Cesar Rios-Navarro, Elena de Dios, Jose M Morales, Jose Gavara, Nerea Perez-Sole, Ana Diaz, Gema Minana, Remedios Segura-Sabater, Clara Bonanad, Antoni Bayés-Genis, Oliver Husser, Jose V Monmeneu, Maria P Lopez-Lereu, Julio Nunez, Francisco J Chorro, Amparo Ruiz-Sauri, Vicente Bodi, Daniel Monleon

Delayed enhancement cardiovascular magnetic resonance (MR) is the gold-standard for non-invasive assessment after myocardial infarction (MI). MR microscopy (MRM) provides a level of detail comparable to the macro objective of light microscopy. We used MRM and correlative histopathology to identify infarct and remote tissue in contrast agent-free multi-sequence MRM in swine MI hearts. One control group (n = 3 swine) and two experimental MI groups were formed: 90 min of ischemia followed by 1 week (acute MI = 6 swine) or 1 month (chronic MI = 5 swine) reperfusion. Representative samples of each heart were analysed by contrast agent-free multi-sequence (T1-weighting, T2-weighting, T2*-weighting, T2-mapping, and T2*-mapping). MRM was performed in a 14-Tesla vertical axis imager (Bruker-AVANCE 600 system). Images from MRM and the corresponding histopathological stained samples revealed differences in signal intensities between infarct and remote areas in both MI groups (p-value < 0.001). The multivariable models allowed us to precisely classify regions of interest (acute MI: specificity 92% and sensitivity 80%; chronic MI: specificity 100% and sensitivity 98%). Probabilistic maps based on MRM images clearly delineated the infarcted regions. As a proof of concept, these results illustrate the potential of MRM with correlative histopathology as a platform for exploring novel contrast agent-free MR biomarkers after MI.

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2,3,5-Triphenyl-tetrazolium chloride solution, suitable for microbiology, Filter sterilized solution that is recommended for the detection of microbial growth based on reduction of TTC