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Molecular cloning and functional expression of a phospholipase D from cabbage (Brassica oleracea var. capitata).

Biochimica et biophysica acta (1999-04-02)
D U Kim, T Y Roh, J Lee, J Y Noh, Y J Jang, K L Hoe, H S Yoo, M U Choi
ABSTRACT

We cloned and expressed a full-length cDNA encoding a phospholipase D of type alpha (PLDalpha) from cabbage. Analysis of the cDNA predicted an 812-amino-acid protein of 92.0 kDa. The deduced amino acid sequence of cabbage PLD has 83% and 80% identity with Arabidopsis PLDalpha and castor bean PLD, respectively. Expression of this cDNA clone in E. coli shows a functional PLD activity similar to that of the natural PLD.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Phospholipase D from cabbage, Type IV, lyophilized powder, ≥100 units/mg solid

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