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  • The YEATS Domain Histone Crotonylation Readers Control Virulence-Related Biology of a Major Human Pathogen.

The YEATS Domain Histone Crotonylation Readers Control Virulence-Related Biology of a Major Human Pathogen.

Cell reports (2020-04-23)
Qi Wang, Jiyoti Verma, Nikolina Vidan, Yanan Wang, Timothy M Tucey, Tricia L Lo, Paul F Harrison, Michael See, Angavai Swaminathan, Karl Kuchler, Michael Tscherner, Jiangning Song, David R Powell, Mary Sopta, Traude H Beilharz, Ana Traven
ABSTRACT

Identification of multiple histone acylations diversifies transcriptional control by metabolism, but their functions are incompletely defined. Here we report evidence of histone crotonylation in the human fungal pathogen Candida albicans. We define the enzymes that regulate crotonylation and show its dynamic control by environmental signals: carbon sources, the short-chain fatty acids butyrate and crotonate, and cell wall stress. Crotonate regulates stress-responsive transcription and rescues C. albicans from cell wall stress, indicating broad impact on cell biology. The YEATS domain crotonylation readers Taf14 and Yaf9 are required for C. albicans virulence, and Taf14 controls gene expression, stress resistance, and invasive growth via its chromatin reader function. Blocking the Taf14 C terminus with a tag reduced virulence, suggesting that inhibiting Taf14 interactions with chromatin regulators impairs function. Our findings shed light on the regulation of histone crotonylation and the functions of the YEATS proteins in eukaryotic pathogen biology and fungal infections.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sodium butyrate, 98%
Sigma-Aldrich
Trichostatin A, ≥98% (HPLC), from Streptomyces sp.
Sigma-Aldrich
Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in goat, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Crotonic acid, 98%