• Home
  • Search Results
  • Multiplexed expansion microscopy of the brain through fluorophore screening.

Multiplexed expansion microscopy of the brain through fluorophore screening.

Methods (San Diego, Calif.) (2019-07-22)
Kyeongbae Min, In Cho, Myunghwan Choi, Jae-Byum Chang
ABSTRACT

Super-resolution microscopy techniques have been widely adopted in biological sciences. Recently, a new super-resolution microscopy technique, called expansion microscopy (ExM) has been developed. In this technique, biomolecules inside specimens are first labeled with fluorophores, followed by in-situ hydrogel synthesis and physical expansion of the specimens. Image quality, including brightness and signal-to-noise ratio, depends on the extent to which fluorophores have bleached during the in-situ hydrogel synthesis process. In this work, we compared the fluorescence signal brightness of more than 20 fluorophores, after expansion, to identify the best fluorophore set for 4-color expansion microscopy imaging. In addition, we achieved 5-color multiplexed expansion microscopy by photo-bleaching one of the four fluorophores and re-staining thereafter.

MATERIALS
Product Number
Brand
Product Description

Grace Bio-Labs CultureWell removable chambered coverglass, size 16 wells , Round
Sigma-Aldrich
Dimethyl sulfoxide, Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
Sigma-Aldrich
Triton X-100, laboratory grade
Sigma-Aldrich
DAPI, for nucleic acid staining
Sigma-Aldrich
Ammonium persulfate, for molecular biology, suitable for electrophoresis, ≥98%
Sigma-Aldrich
Guanidine hydrochloride, for molecular biology, ≥99%
Sigma-Aldrich
Poly-L-lysine solution, 0.1 % (w/v) in H2O
Sigma-Aldrich
Calcium chloride, anhydrous, granular, ≤7.0 mm, ≥93.0%
Sigma-Aldrich
Acrylamide, for molecular biology, ≥99% (HPLC)
Sigma-Aldrich
Glycine, BioUltra, for molecular biology, ≥99.0% (NT)
Sigma-Aldrich
Sodium borohydride, purum p.a., ≥96% (gas-volumetric)
Sigma-Aldrich
4-Hydroxy-TEMPO, 97%
Sigma-Aldrich
Sodium acrylate, 97%
Sigma-Aldrich
N,N′-Methylenebisacrylamide, powder, for molecular biology, suitable for electrophoresis, ≥99.5%
Sigma-Aldrich
Sodium chloride, BioUltra, for molecular biology, ≥99.5% (AT)
Sigma-Aldrich
2,2,6,6-Tetramethyl-4-piperidinol, 98%

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.