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Tubulin polyglutamylation is a general traffic-control mechanism in hippocampal neurons.

Journal of cell science (2020-01-15)
Satish Bodakuntla, Anne Schnitzler, Cristopher Villablanca, Christian Gonzalez-Billault, Ivan Bieche, Carsten Janke, Maria M Magiera

Neurons are highly complex cells that heavily rely on intracellular transport to distribute a range of functionally essential cargoes within the cell. Post-translational modifications of tubulin are emerging as mechanisms for regulating microtubule functions, but their impact on neuronal transport is only marginally understood. Here, we have systematically studied the impact of post-translational polyglutamylation on axonal transport. In cultured hippocampal neurons, deletion of a single deglutamylase, CCP1 (also known as AGTPBP1), is sufficient to induce abnormal accumulation of polyglutamylation, i.e. hyperglutamylation. We next investigated how hyperglutamylation affects axonal transport of a range of functionally different neuronal cargoes: mitochondria, lysosomes, LAMP1 endosomes and BDNF vesicles. Strikingly, we found a reduced motility for all these cargoes, suggesting that polyglutamylation could act as a regulator of cargo transport in neurons. This, together with the recent discovery that hyperglutamylation induces neurodegeneration, makes it likely that perturbed neuronal trafficking could be one of the central molecular causes underlying this novel type of degeneration.This article has an associated First Person interview with the first author of the paper.

Product Number
Product Description

DL-Dithiothreitol, for molecular biology, ≥98% (HPLC), ≥99% (titration)
Sodium chloride, for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)
Trizma® base, Primary Standard and Buffer, ≥99.9% (titration), crystalline
REDExtract-N-Amp Tissue PCR Kit, sufficient for 10 reactions, sufficient for 100 reactions, sufficient for 1000 reactions, hotstart, dNTPs included
Monoclonal Anti-Tubulin, Acetylated antibody produced in mouse, clone 6-11B-1, ascites fluid
Anti-Tubulin Antibody, Detyrosinated, Chemicon®, from rabbit