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In vitro static digestion reveals how plant proteins modulate model infant formula digestibility.

Food research international (Ottawa, Ont.) (2020-03-12)
Linda Le Roux, Raphaël Chacon, Didier Dupont, Romain Jeantet, Amélie Deglaire, Françoise Nau
ABSTRACT

Infant formulas (IFs) are the key nutritional source for infants who cannot be breastfed. There is currently a growing interest in these sensitive products in order to control their quality and to design their composition with regard to nutritional balance. In a context of sustainable development and increasing growth of the world population, it seems essential to search for alternative to animal protein in food today. Plant proteins offer interesting nutritional and functional benefits thanks to the latest improvement through research and development. In this context, five model IFs were developed with identical composition, except that 50% of the proteins were either whey proteins in the "milk-reference IF", pea, faba bean, rice or potato proteins in the four "plant IFs" tested. The IFs were evaluated using an in vitro static gastro-intestinal model simulating infant conditions. The protein hydrolysis degree (DH) and the amino acid bioaccessibility (AAB) were used as indicators of protein digestibility. Results showed that both DH and AAB were very similar between the milk-reference IF, pea and faba bean IFs, but significantly lower for the rice and potato IFs. This study provides new insights into the impact of protein sources on IF digestibility.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Pancreatin from porcine pancreas, 8 × USP specifications
Sigma-Aldrich
Pepsin from porcine gastric mucosa, lyophilized powder, ≥2,500 units/mg protein (E1%/280)
Sigma-Aldrich
Pepstatin A, microbial, ≥90% (HPLC)
Sigma-Aldrich
Bile extract porcine
Supelco
Pefabloc® SC, analytical standard
Supelco
Amino acid standards, physiological, analytical standard, acidics, neutrals, and basics