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Constructing Cross-Linked Nanofibrous Scaffold via Dual-Enzyme-Instructed Hierarchical Assembly.

Langmuir : the ACS journal of surfaces and colloids (2020-05-19)
Shijin Zhang, William Cortes, Ye Zhang
ABSTRACT

To explore the potential of step-by-step assembly in the fabrication of biological materials, we designed and synthesized two peptide-based molecules for enzyme-instructed hierarchical assembly. Upon the treatment of alkaline phosphatase, one molecule undergoes enzyme-instructed self-assembly forming uniformed nanofibers. The other one that can self-assemble into vesicles undergoes enzyme-induced transformation of self-assembly converting vesicles into irregular aggregates upon the treatment of carboxylesterase. Coadministration of two enzymes to a mixture of these two molecules in a stage-by-stage fashion leads to a physically knotted nanofibrous scaffold that is applicable as a nanostructured matrix for cell culture.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Esterase from rabbit liver, lyophilized powder, ≥30 units/mg protein

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