Improving extracellular vesicles visualization: From static to motion.

Scientific reports (2020-04-18)
Pablo Reclusa, Peter Verstraelen, Simona Taverna, Muthukumar Gunasekaran, Marzia Pucci, Isabel Pintelon, Nathalie Claes, Diego de Miguel-Pérez, Riccardo Alessandro, Sara Bals, Sunjay Kaushal, Christian Rolfo
ABSTRACT

In the last decade, extracellular vesicles (EVs) have become a hot topic. The findings on EVs content and effects have made them a major field of interest in cancer research. EVs, are able to be internalized through integrins expressed in parental cells, in a tissue specific manner, as a key step of cancer progression and pre-metastatic niche formation. However, this specificity might lead to new opportunities in cancer treatment by using EVs as devices for drug delivery. For future applications of EVs in cancer, improved protocols and methods for EVs isolation and visualization are required. Our group has put efforts on developing a protocol able to track the EVs for in vivo internalization analysis. We showed, for the first time, the videos of labeled EVs uptake by living lung cancer cells.

MATERIALS
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Brand
Product Description

Sigma-Aldrich
PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labeling, Distributed for Phanos Technologies