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Room temperature shipment does not affect the biological activity of pluripotent stem cell-derived retinal organoids.

PloS one (2020-06-02)
Maria Georgiou, Valeria Chichagova, Gerrit Hilgen, Birthe Dorgau, Evelyne Sernagor, Lyle Armstrong, Majlinda Lako
ABSTRACT

The generation of laminated and light responsive retinal organoids from induced pluripotent stem cells (iPSCs) provides a powerful tool for the study of retinal diseases and drug discovery and a robust platform for cell-based therapies. The aim of this study is to investigate whether retinal organoids can retain their morphological and functional characteristics upon storage at room temperature (RT) conditions and shipment by air using a commercially available container that maintains the environment at ambient temperature. Morphological analysis and measurements of neuroepithelial thickness revealed no differences between control, RT incubated and shipped organoids. Similarly immunohistochemical analysis showed no differences in cell type composition and position within the laminated retinal structure. All groups showed a similar response to light, suggesting that the biological function of retinal organoids was not affected by RT storage or shipment. These findings provide an advance in transport of ready-made retinal organoids, increasing their availability to many research and pharma labs worldwide and facilitating cross-collaborative research.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Bovine Serum Albumin, lyophilized powder, BioReagent, suitable for cell culture
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Retinoic acid, ≥98% (HPLC), powder
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CHIR99021, ≥98% (HPLC)
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1-Thioglycerol, liquid, BioReagent, suitable for cell culture, ≥97% (titration)
Sigma-Aldrich
bisBenzimide H 33342 trihydrochloride, ≥98% (HPLC and TLC)
Sigma-Aldrich
Taurine, suitable for cell culture, meets USP testing specifications
Sigma-Aldrich
9-cis-Retinal, vitamin A analog